Summary of Study ST000387

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000303. The data can be accessed directly via it's Project DOI: 10.21228/M8W593 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000387
Study TitleChanges in the metabalome and lipidome in response to exercise training
Study TypeHERITAGE (HEalth, RIsk factors, exercise Training And GEnetics) family study
Study SummaryThe overall objective of the Heritage Family Study is to study the role of the genotype in cardiovascular, metabolic, and hormonal responses to aerobic exercise training and the contribution of regular exercise to changes in several cardiovascular disease and diabetes risk factors. The study cohort used in this analysis was derived from the pool of 473 Caucasian subjects (230 male and 243 female) from 99 nuclear families who completed ≥58 of the prescribed 60 exercise-training sessions. Utilizing a subsample of this Caucasian cohort, we selected family members from the Quebec center (N=125) to assess the metabolome and lipidome of circulating plasma under two well-defined environmental conditions, the pre- and post-training conditions.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
AddressHealth Sciences Drive, Davis, California, 95616, USA
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2016-04-26
Num Groups3
Total Subjects250
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-04-29
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M8W593
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000303
Project DOI:doi: 10.21228/M8W593
Project Title:Changes in the metabalome and lipidome in response to exercise training
Project Type:HERITAGE (HEalth, RIsk factors, exercise Training And GEnetics) family study
Project Summary:The overall objective of the Heritage Family Study is to study the role of the genotype in cardiovascular, metabolic, and hormonal responses to aerobic exercise training and the contribution of regular exercise to changes in several cardiovascular disease and diabetes risk factors. The study cohort used in this analysis was derived from the pool of 473 Caucasian subjects (230 male and 243 female) from 99 nuclear families who completed ≥58 of the prescribed 60 exercise-training sessions. Utilizing a subsample of this Caucasian cohort, we selected family members from the Quebec center (N=125) to assess the metabolome and lipidome of circulating plasma under two well-defined environmental conditions, the pre- and post-training conditions.
Institute:University of California, Davis
Department:Genome and Biomedical Sciences Facility
Laboratory:WCMC Metabolomics Core
Last Name:Fiehn
First Name:Oliver
Address:Health Sciences Drive, Davis, California, 95616, USA
Email:ofiehn@ucdavis.edu
Phone:(530) 754-8258
Funding Source:NIH U24DK097154

Subject:

Subject ID:SU000408
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA017853Sarz125_51741post_241_CSHMS.dPost-Exercise
SA017854Sarz126_51224post_204_CSHMS.dPost-Exercise
SA017855Sarz134_50783post_148_CSHMS.dPost-Exercise
SA017856Sarz123_51725post_235_CSHMS.dPost-Exercise
SA017857Sarz128_51744post_243_CSHMS.dPost-Exercise
SA017858Sarz120_51201post_198_CSHMS.dPost-Exercise
SA017859Sarz111_51685post_229_CSHMS.dPost-Exercise
SA017860Sarz116_51164post_196_CSHMS.dPost-Exercise
SA017861Sarz119_51542post_223_CSHMS.dPost-Exercise
SA017862Sarz137_51686post_230_CSHMS.dPost-Exercise
SA017863Sarz121_51112post_189_CSHMS.dPost-Exercise
SA017864Sarz138_50963post_170_CSHMS.dPost-Exercise
SA017865Sarz150_50585post_130_CSHMS.dPost-Exercise
SA017866Sarz152_50903post_158_CSHMS.dPost-Exercise
SA017867Sarz154_50781post_146_CSHMS.dPost-Exercise
SA017868Sarz155_51004post_186_CSHMS.dPost-Exercise
SA017869Sarz149_51163post_195_CSHMS.dPost-Exercise
SA017870Sarz148_51486post_221_CSHMS.dPost-Exercise
SA017871Sarz142_50983post_179_CSHMS.dPost-Exercise
SA017872Sarz144_50881post_151_CSHMS.dPost-Exercise
SA017873Sarz146_50985post_181_CSHMS.dPost-Exercise
SA017874Sarz110_51204post_201_CSHMS.dPost-Exercise
SA017875Sarz109_51116post_192_CSHMS.dPost-Exercise
SA017876Sarz82_50661post_132_CSHMS.dPost-Exercise
SA017877Sarz83_50586post_131_CSHMS.dPost-Exercise
SA017878Sarz86_51765post_250_CSHMS.dPost-Exercise
SA017879Sarz88_51221post_202_CSHMS.dPost-Exercise
SA017880Sarz77_51222post_203_CSHMS.dPost-Exercise
SA017881Sarz75_50884post_154_CSHMS.dPost-Exercise
SA017882Sarz71_51571post_236_CSHMS.dPost-Exercise
SA017883Sarz72_50901post_156_CSHMS.dPost-Exercise
SA017884Sarz74_50904post_159_CSHMS.dPost-Exercise
SA017885Sarz89_50925post_165_CSHMS.dPost-Exercise
SA017886Sarz91_51243post_208_CSHMS.dPost-Exercise
SA017887Sarz98_50672post_139_CSHMS.dPost-Exercise
SA017888Sarz103_50582post_127_CSHMS.dPost-Exercise
SA017889Sarz105_50662post_133_CSHMS.dPost-Exercise
SA017890Sarz106_51203post_200_CSHMS.dPost-Exercise
SA017891Sarz96_50966post_173_CSHMS.dPost-Exercise
SA017892Sarz95_50885post_155_CSHMS.dPost-Exercise
SA017893Sarz92_50961post_168_CSHMS.dPost-Exercise
SA017894Sarz93_51161post_193_CSHMS.dPost-Exercise
SA017895Sarz94_51244post_209_CSHMS.dPost-Exercise
SA017896Sarz156_51483post_218_CSHMS.dPost-Exercise
SA017897Sarz157_51721post_231_CSHMS.dPost-Exercise
SA017898Sarz217_50902post_157_CSHMS.dPost-Exercise
SA017899Sarz219_51543post_224_CSHMS.dPost-Exercise
SA017900Sarz220_50955post_176_CSHMS.dPost-Exercise
SA017901Sarz221_50664post_135_CSHMS.dPost-Exercise
SA017902Sarz216_51541post_222_CSHMS.dPost-Exercise
SA017903Sarz215_51464post_214_CSHMS.dPost-Exercise
SA017904Sarz206_51111post_188_CSHMS.dPost-Exercise
SA017905Sarz209_51575post_239_CSHMS.dPost-Exercise
SA017906Sarz213_51001post_183_CSHMS.dPost-Exercise
SA017907Sarz223_50883post_153_CSHMS.dPost-Exercise
SA017908Sarz224_51745post_244_CSHMS.dPost-Exercise
SA017909Sarz237_50952post_182_CSHMS.dPost-Exercise
SA017910Sarz239_51746post_245_CSHMS.dPost-Exercise
SA017911Sarz246_50965post_172_CSHMS.dPost-Exercise
SA017912Sarz250_51761post_246_CSHMS.dPost-Exercise
SA017913Sarz236_50981post_177_CSHMS.dPost-Exercise
SA017914Sarz234_51002post_184_CSHMS.dPost-Exercise
SA017915Sarz225_51165pre_072_CSHMS.dPost-Exercise
SA017916Sarz229_51113post_190_CSHMS.dPost-Exercise
SA017917Sarz233_51242post_207_CSHMS.dPost-Exercise
SA017918Sarz204_50705post_145_CSHMS.dPost-Exercise
SA017919Sarz203_51681post_226_CSHMS.dPost-Exercise
SA017920Sarz170_50702post_143_CSHMS.dPost-Exercise
SA017921Sarz172_51225post_205_CSHMS.dPost-Exercise
SA017922Sarz175_50924post_164_CSHMS.dPost-Exercise
SA017923Sarz177_50951post_167_CSHMS.dPost-Exercise
SA017924Sarz166_51245post_210_CSHMS.dPost-Exercise
SA017925Sarz164_51762post_247_CSHMS.dPost-Exercise
SA017926Sarz159_51241post_206_CSHMS.dPost-Exercise
SA017927Sarz160_50665post_136_CSHMS.dPost-Exercise
SA017928Sarz162_51683post_228_CSHMS.dPost-Exercise
SA017929Sarz179_50921post_161_CSHMS.dPost-Exercise
SA017930Sarz182_51485post_220_CSHMS.dPost-Exercise
SA017931Sarz193_50584post_129_CSHMS.dPost-Exercise
SA017932Sarz197_50663post_134_CSHMS.dPost-Exercise
SA017933Sarz201_51465post_215_CSHMS.dPost-Exercise
SA017934Sarz202_51722post_232_CSHMS.dPost-Exercise
SA017935Sarz192_50666post_137_CSHMS.dPost-Exercise
SA017936Sarz191_50962post_169_CSHMS.dPost-Exercise
SA017937Sarz183_51461post_211_CSHMS.dPost-Exercise
SA017938Sarz184_50954post_175_CSHMS.dPost-Exercise
SA017939Sarz187_50922post_162_CSHMS.dPost-Exercise
SA017940Sarz68_51165post_197_CSHMS.dPost-Exercise
SA017941Sarz113_50704post_144_CSHMS.dPost-Exercise
SA017942Sarz31_51481post_216_CSHMS.dPost-Exercise
SA017943Sarz47_50782post_147_CSHMS.dPost-Exercise
SA017944Sarz32_50926post_166_CSHMS.dPost-Exercise
SA017945Sarz11_51574post_238_CSHMS.dPost-Exercise
SA017946Sarz50_51484post_219_CSHMS.dPost-Exercise
SA017947Sarz30_50583post_128_CSHMS.dPost-Exercise
SA017948Sarz54_51576post_240_CSHMS.dPost-Exercise
SA017949Sarz12_50984post_180_CSHMS.dPost-Exercise
SA017950Sarz39_50784post_149_CSHMS.dPost-Exercise
SA017951Sarz52_51114post_191_CSHMS.dPost-Exercise
SA017952Sarz10_51743post_242_CSHMS.dPost-Exercise
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Collection:

Collection ID:CO000402
Collection Summary:Whole blood was processed into serum and plasma and stored at 80C
Collection Protocol Filename:StudyDesign-MarkSarzynski-72315.pdf
Sample Type:Blood
Blood Serum Or Plasma:Plasma

Treatment:

Treatment ID:TR000422
Treatment Summary:Before and after 20 weeks of endurance exercise training: samples were taken at baseline (N=125) and postexercise training (N=125) in the same individuals
Treatment Protocol Filename:StudyDesign-MarkSarzynski-72315.pdf

Sample Preparation:

Sampleprep ID:SP000415
Sampleprep Summary:1) Degas both “MeOH with QCmix” and MTBE with Cholesterol Ester 22:1 bysonication 2) Store solvents in the –20°C freezer to pre-chill 3) Thaw each 20 μL plasma aliquot at room temperature. Once thawed (~10min) place liquid plasma samples on ice. 4) Add 225 μL cold “MeOH with QC mix” (see SOP “QC mix for LC-MS lipid analysis”). Keep MeOH on ice during extraction 5) Vortex each sample for 10s, keeping the rest on ice during all the extraction. 6) Add 750 μL of cold MTBE with 22:1 CE, keep MTBE on ice during extraction Vortex for 10s 7) Shake for 6min at 4°C in the orbital mixer. Add 188 μL room temperature LC/MS grade water. 8)Vortex for 20 s 9) Centrifuge for 2 min @ 14,000 rcf (12300 rpm) 10) Remove supernatant, splitting into two aliquots of 350 μL, keeping one at –20°C for backup 11) Dry samples to complete dryness in the speed vacuum concentration system
Sampleprep Protocol Filename:SOP_Lipid_Extraction_Plasma_06242013 (1).pdf
Processing Method:Homogenization

Combined analysis:

Analysis ID AN000622 AN000623
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 6530 Agilent 6550
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6550 QTOF
Ion Mode POSITIVE NEGATIVE
Units counts counts

Chromatography:

Chromatography ID:CH000447
Methods Filename:Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf
Instrument Name:Agilent 6530
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Pressure:450-850 bar
Column Temperature:65 C
Flow Gradient:15% B to 99%B
Flow Rate:0.6 mL/min
Internal Standard:See data dictionary
Retention Time:See data dictionary
Sample Injection:1.67 uL
Solvent A:60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate
Analytical Time:13 min
Capillary Voltage:3500 V
Time Program:15 min
Weak Wash Solvent Name:Isopropanol
Strong Wash Solvent Name:Isopropanol
Target Sample Temperature:Autosampler temp 4 C
Randomization Order:Excel generated
Chromatography Type:Reversed phase
  
Chromatography ID:CH000448
Methods Filename:Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf
Instrument Name:Agilent 6550
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Pressure:450-850 bar
Column Temperature:65 C
Flow Gradient:15% B to 99%B
Flow Rate:0.6 mL/min
Internal Standard:see data dictionary
Retention Time:see data dictionary
Sample Injection:5 uL
Solvent A:95% water/5% acetonitrile; 0.1% acetic acid
Solvent B:100% acetonitrile; 0.1% acetic acid
Analytical Time:13 min
Capillary Voltage:3500 V
Time Program:15 min
Weak Wash Solvent Name:Isopropanol
Strong Wash Solvent Name:Isopropanol
Target Sample Temperature:Autosampler temp 4 C
Randomization Order:Excel generated
Chromatography Type:Reversed phase

MS:

MS ID:MS000555
Analysis ID:AN000622
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3500
Collision Gas:Nitrogen
Dry Gas Flow:8 L/min
Dry Gas Temp:325 C
Fragment Voltage:120 V
Fragmentation Method:Auto MS/MS
Ion Source Temperature:325 C
Ion Spray Voltage:1000 V
Ionization:Pos
Precursor Type:Intact Molecule
Reagent Gas:Nitrogen
Source Temperature:325 C
Dataformat:.d
Desolvation Gas Flow:11 L/min
Desolvation Temperature:350 C
Nebulizer:35 psig
Octpole Voltage:750 V
Resolution Setting:extended dynamic range
Scan Range Moverz:60-1700 Da
Scanning Cycle:2 Hz
Scanning Range:60-1700 Da
Skimmer Voltage:65 V
  
MS ID:MS000556
Analysis ID:AN000623
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:3500
Collision Gas:Nitrogen
Dry Gas Flow:13 L/min
Dry Gas Temp:200 C
Fragment Voltage:175 V
Fragmentation Method:Auto MS/MS
Ion Source Temperature:325 C
Ion Spray Voltage:1000
Ionization:Neg
Dataformat:.d
Desolvation Gas Flow:11 L/min
Desolvation Temperature:350 C
Nebulizer:35 psig
Octpole Voltage:750 V
Scan Range Moverz:60-1700 Da
Scanning Cycle:2Hz
Scanning Range:60-1700
Skimmer Voltage:65 V
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