Summary of Study ST000398

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000311. The data can be accessed directly via it's Project DOI: 10.21228/M8G020 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000398
Study TitleMetabolic profiling of maternal urine can aid clinical management of Gestational Diabetes Mellitus (GDM)
Study TypeSearch for non-treated and treated GDM biomarkers in urine
Study SummaryNMR metabolomics study of maternal urine of 1) GDM women at the time of diagnosis and before treatment, to define the urine metabolic profile of untreated GDM, and of 2) GDM women treated using diet control alone or with the addition of insulin, to identify treatment-resistant and treatment-responsive metabolic pathways and, hence, evaluate treatment efficacy, and 3) GDM treatment prediction at the time of diagnosis, with the aim of finding potential predictive markers of future treatment requirements based on each individual metabotype.
Institute
University of Aveiro
DepartmentCICECO-Department of Chemistry
Last NameGil
First NameAna
AddressCICECO-Department of Chemistry, University of Aveiro
Emailagil@ua.pt
Phonenone
Submit Date2016-04-20
Num Groups5
Total Subjects98
Analysis Type DetailNMR
Release Date2016-06-18
Release Version1
Ana Gil Ana Gil
https://dx.doi.org/10.21228/M8G020
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000311
Project DOI:doi: 10.21228/M8G020
Project Title:Urine metabolomics for the study of Gestational Diabetes Mellitus
Project Summary:A urine NMR metabolomics study using a variable selection methodology to reduce uninformative variability enables a metabolic signature of gestational diabetes mellitus (GDM) to be identified at the time of diagnosis. This signature comprises relevant changes in 12 NMR metabolites/resonances and qualitative variations in a number of additional metabolites.
Institute:University of Aveiro
Department:CICECO-Department of Chemistry
Last Name:Gil
First Name:Ana
Address:CICECO-Department of Chemistry, University of Aveiro
Email:agil@ua.pt
Phone:none

Subject:

Subject ID:SU000419
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:18-41 years
Gender:Female
Human Race:Caucasian
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id group ID Treatment duration
SA019107PU81_09_1_1Control group 1 NA
SA019108PU848_06_1_1Control group 1 NA
SA019109PU3T9_11_31gw_1_1Control group 1 NA
SA019110PU640_10_22gw_1_1Control group 1 NA
SA019111PU2T2_10_16gw_1_1Control group 1 NA
SA019112PU895_06_1_1Control group 1 NA
SA019113PU3T25_11_32gw_1_1Control group 2 NA
SA019114PU3T27_11_38gw_2_1Control group 2 NA
SA019115PU3T26_11_33gw_1_1Control group 2 NA
SA019116PU3T24_11_38gw_1_1Control group 2 NA
SA019117PU3T22_11_37gw_1_1Control group 2 NA
SA019118PU3T17_11_37gw_1_1Control group 2 NA
SA019119PU3T11_11_39gw_1_1Control group 2 NA
SA019120PU3T18_11_39gw_1_1Control group 2 NA
SA019121PU3T1_12_1_1Control group 2 NA
SA019122PU3T21_11_37gw_1_1Control group 2 NA
SA019123PU3T30_11_35gw_1_1Control group 2 NA
SA019124PU3T43_11_1_1Control group 2 NA
SA019125PU3T8_11_29gw_1_1Control group 2 NA
SA019126PU3T9_11_31gw_2_1Control group 2 NA
SA019127PU895_06_2_1Control group 2 NA
SA019128PU1129_09_1_1Control group 2 NA
SA019129PU3T5_11_32gw_1_1Control group 2 NA
SA019130PU3T51_11_2_1Control group 2 NA
SA019131PU1253_09_26gw_1_1Control group 2 NA
SA019132PU3T3_11_39gw_1_1Control group 2 NA
SA019133PU3T10_11_35gw_1_1Control group 2 NA
SA019134PU3T34_11_31gw_1_1Control group 2 NA
SA019135PU3T39_11_1_1Control group 2 NA
SA019136PU3T2_11_32gw_1_1Control group 2 NA
SA019137PU3T11_10_26gw_1_1Control group 2 NA
SA019138PU3T32_11_32gw_1_1Control group 2 NA
SA019139PU3T8_12_1_1Control group 2 NA
SA019140PU1288_09_1_1Control group 2 NA
SA019141PU3T7_12_1_1Control group 2 NA
SA019142PU3T6_10_32gw_1_1Control group 2 NA
SA019143PU3T46_11_1_1Diet treated-GDM 11
SA019144PU3T40_11_1_1Diet treated-GDM 11
SA019145PU2T10_11_1_1Diet treated-GDM 12
SA019146PU3T4_12_1_1Diet treated-GDM 13
SA019147PUsc3410_1_1Diet treated-GDM 2
SA019148PU3T2_12_1_1Diet treated-GDM 2
SA019149PU3T5_10_1_1Diet treated-GDM 2
SA019150PUsc2310_1_1Diet treated-GDM 2
SA019151PUsc3010_1_1Diet treated-GDM 2
SA019152PUsc2610_1_1Diet treated-GDM 2
SA019153PU3T14_11_1_1Diet treated-GDM 3
SA019154PU2T811_1_1Diet treated-GDM 3
SA019155PU2T11_11_3_1Diet treated-GDM 3
SA019156PUsc2210_1_1Diet treated-GDM 3
SA019157PUsc2710_3_1Diet treated-GDM 3
SA019158PUsc1010_3_1Diet treated-GDM 3
SA019159PUsc2410_1_1Diet treated-GDM 3
SA019160PUsc3610_1_1Diet treated-GDM 4
SA019161PUsc2110_1_1Diet treated-GDM 4
SA019162PU668_09_1_1Diet treated-GDM 4
SA019163PUsc310_2_1Diet treated-GDM 5
SA019164PUsc410_2_1Diet treated-GDM 5
SA019165PUsc1_11_1_1Diet treated-GDM 6
SA019166PUsc2010_1_1Diet treated-GDM 7
SA019167PUsc710_3_1Diet treated-GDM 7
SA019168PUsc3210_1_1Diet treated-GDM 7
SA019169PUsc2810_1_1Diet treated-GDM 7
SA019170PU3T44_11_1_1Diet treated-GDM 9
SA019171PUsc110_2_1Insulin treated-GDM 12
SA019172PU3T41_11Insulin treated-GDM 14
SA019173PUsc910_2_1Insulin treated-GDM 22
SA019174PUsc2510_1_1Insulin treated-GDM 3
SA019175PU3T38_11_1_1Insulin treated-GDM 6
SA019176PUsc210_2_1Insulin treated-GDM 6
SA019177PU1106_09_Ins_2T_1_1Insulin treated-GDM 7
SA019178PUsc610_2_1Insulin treated-GDM 9
SA019179U_Dx4_15_1_1Non treated-GDM, Diet-requiring NA
SA019180U_Dx27_15_1_1Non treated-GDM, Diet-requiring NA
SA019181U_Dx5_15_1_1Non treated-GDM, Diet-requiring NA
SA019182PU_HDD9_11_1_1Non treated-GDM, Diet-requiring NA
SA019183U_Dx11_15_1_1Non treated-GDM, Diet-requiring NA
SA019184PU_HDD8_11_1_1Non treated-GDM, Diet-requiring NA
SA019185PU_HDD5_12_1_1Non treated-GDM, Diet-requiring NA
SA019186PU_HDD3_11_1_1Non treated-GDM, Diet-requiring NA
SA019187PU_HDD3_12_1_1Non treated-GDM, Diet-requiring NA
SA019188U_Dx26_15_1_1Non treated-GDM, Diet-requiring NA
SA019189U_Dx13_15_1_1Non treated-GDM, Diet-requiring NA
SA019190U_Dx25_15_1_1Non treated-GDM, Diet-requiring NA
SA019191PU_HDD5_11_1_1Non treated-GDM, Insulin-requiring NA
SA019192U_Dx23_15_1_1Non treated-GDM, Insulin-requiring NA
SA019193PU_HDD10_11_1_1Non treated-GDM, Insulin-requiring NA
SA019194PU_HDD1_11_1_1Non treated-GDM, Insulin-requiring NA
SA019195PU_HDD2_12_1_1Non treated-GDM, Insulin-requiring NA
SA019196PU_HDD1_12_1_1Non treated-GDM, Insulin-requiring NA
Showing results 1 to 90 of 90

Collection:

Collection ID:CO000413
Collection Summary:Urine samples were collected at routine medical appointments and diabetes counselling appointments, for independent groups of women in their 2nd and 3rd trimesters of pregnancy. Samples were collected under ethical approval of the Hospital Center of Coimbra (Refs.18/04 and 29/09) and informed consents were obtained from each woman.
Sample Type:Urine
Collection Location:Maternity Bissaya Barreto, Coimbra, Portugal
Collection Time:Non-fasting, during morning
Volumeoramount Collected:50 mL
Storage Conditions:-80ºC
Additives:No

Treatment:

Treatment ID:TR000433
Treatment Summary:Subjects were treated with diet or insulin
Treatment Compound:Insulin

Sample Preparation:

Sampleprep ID:SP000426
Sampleprep Summary:Before analysis, samples were thawed and 800 μL were centrifuged (4500g, 5 min). Then, 60 μL of 1.5 M phosphate buffer pH 7 in D2O, 0.1% Na+/3-trimethylsilyl-propionate (TSP) were added to 540 μL of supernatant, followed by pH readjustment to 7.00±0.02 with KOD (4 M) or DCl (4 M). The mixture was centrifuged (4500 g, 5 min) and 550 μL transferred to 5 mm NMR tubes.

Analysis:

Analysis ID:AN000635
Laboratory Name:CICECO metabolomics group
Analysis Type:NMR
Analysis Protocol File:noesypr1d
Software Version:Topspin 3.2
Num Factors:21

NMR:

NMR ID:NM000067
Analysis ID:AN000635
Instrument Name:Bruker Avance DRX 500
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
NMR Comments:Standard experiment (noesypr1d)
Field Frequency Lock:Deuterium
Spectrometer Frequency:500 MHz
NMR Probe:BBI
NMR Solvent:D2O+H2O
NMR Tube Size:5 mm
Shimming Method:Automatic
Pulse Sequence:noesypr1d
Water Suppression:presaturation
Receiver Gain:32
Chemical Shift Ref Cpd:TSP
Temperature:300 K
Number Of Scans:128
Dummy Scans:4
Acquisition Time:3.25 s
Relaxation Delay:32
Spectral Width:20.16 ppm
Num Data Points Acquired:64 k
Real Data Points:64 k
Line Broadening:0.3 Hz
Baseline Correction Method:manual
Chemical Shift Ref Std:TSP (0.0 ppm)
Binned Increment:full resolution
Binned Data Excluded Range:4.60-5.05 ppm
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