Summary of Study ST000408

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000408
Study TitleMetabolomic analysis of oxytocin effects on social deficits in mice (part I)
Study SummaryThe goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in brain.
Institute
University of North Carolina
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-02
Raw Data AvailableYes
Raw Data File Type(s)1r
Analysis Type DetailNMR
Release Date2018-06-05
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8MP4W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000320
Project DOI:doi: 10.21228/M8MP4W
Project Title:Metabolomic analysis of oxytocin effects on social deficits in mice
Project Summary:The goal of this study was to determine the effects of the neuropeptide oxytocin (OT) on metabolomic profiles, using a mouse model of autism-like behavior, the BALB/cByJ inbred strain. We have previously reported that subchronic treatment with OT can lead to persistent reversal of social deficits in BALB/cByJ and other models of autism spectrum disorder (ASD). In this study, mice were given a subchronic regimen with either vehicle or OT, and then evaluated for social approach. At the end of the study, brain and blood were collected for metabolomic analysis. In addition, fecal samples were taken at different time points during the treatment and testing regimen. The results from this project could elucidate mechanisms underlying the prosocial effects of oxytocin, and identify new targets for the development of highly specific oxytocin-related drugs.
Institute:University of North Carolina at Chapel Hill
Department:Department of Psychiatry
Last Name:Moy
First Name:Sheryl
Address:CB# 7146, Chapel Hill, NC 277599
Email:ssmoy@med.unc.edu
Phone:919-966-3082

Subject:

Subject ID:SU000429
Subject Type:Mice
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mice; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Brain region
SA019883B_79Oxytocin Cerb
SA019884B_68Oxytocin Cerb
SA019885B_70Oxytocin Cerb
SA019886B_75Oxytocin Cerb
SA019887B_72Oxytocin Cerb
SA019888B_77Oxytocin Cerb
SA019889B_66Oxytocin Cerb
SA019890B_61Oxytocin Cerb
SA019891B_62Oxytocin Cerb
SA019892B_63Oxytocin Cerb
SA019893B_74Oxytocin Cerb
SA019894B_65Oxytocin Cerb
SA019895B_30Oxytocin Fore
SA019896B_37Oxytocin Fore
SA019897B_39Oxytocin Fore
SA019898B_35Oxytocin Fore
SA019899B_34Oxytocin Fore
SA019900B_32Oxytocin Fore
SA019901B_28Oxytocin Fore
SA019902B_21Oxytocin Fore
SA019903B_23Oxytocin Fore
SA019904B_22Oxytocin Fore
SA019905B_25Oxytocin Fore
SA019906B_26Oxytocin Fore
SA019907B_94Oxytocin Hind
SA019908B_92Oxytocin Hind
SA019909B_95Oxytocin Hind
SA019910B_97Oxytocin Hind
SA019911B_99Oxytocin Hind
SA019912B_90Oxytocin Hind
SA019913B_86Oxytocin Hind
SA019914B_88Oxytocin Hind
SA019915B_81Oxytocin Hind
SA019916B_83Oxytocin Hind
SA019917B_82Oxytocin Hind
SA019918B_85Oxytocin Hind
SA019919B_52Oxytocin Mid
SA019920B_54Oxytocin Mid
SA019921B_59Oxytocin Mid
SA019922B_50Oxytocin Mid
SA019923B_55Oxytocin Mid
SA019924B_57Oxytocin Mid
SA019925B_41Oxytocin Mid
SA019926B_42Oxytocin Mid
SA019927B_43Oxytocin Mid
SA019928B_45Oxytocin Mid
SA019929B_46Oxytocin Mid
SA019930B_48Oxytocin Mid
SA019931B_8Oxytocin OlF
SA019932B_3Oxytocin OlF
SA019933B_14Oxytocin OlF
SA019934B_15Oxytocin OlF
SA019935B_12Oxytocin OlF
SA019936B_2Oxytocin OlF
SA019937B_19Oxytocin OlF
SA019938B_6Oxytocin OlF
SA019939B_1Oxytocin OlF
SA019940B_10Oxytocin OlF
SA019941B_5Oxytocin OlF
SA019942PB_5Pool Pool
SA019943PB_4Pool Pool
SA019944PB_2Pool Pool
SA019945PB_1Pool Pool
SA019946PB_3Pool Pool
SA019947B_78Veh Cerb
SA019948B_80Veh Cerb
SA019949B_73Veh Cerb
SA019950B_76Veh Cerb
SA019951B_64Veh Cerb
SA019952B_67Veh Cerb
SA019953B_69Veh Cerb
SA019954B_71Veh Cerb
SA019955B_24Veh Fore
SA019956B_31Veh Fore
SA019957B_36Veh Fore
SA019958B_40Veh Fore
SA019959B_38Veh Fore
SA019960B_29Veh Fore
SA019961B_27Veh Fore
SA019962B_33Veh Fore
SA019963B_89Veh Hind
SA019964B_100Veh Hind
SA019965B_96Veh Hind
SA019966B_93Veh Hind
SA019967B_91Veh Hind
SA019968B_84Veh Hind
SA019969B_87Veh Hind
SA019970B_98Veh Hind
SA019971B_60Veh Mid
SA019972B_51Veh Mid
SA019973B_49Veh Mid
SA019974B_44Veh Mid
SA019975B_47Veh Mid
SA019976B_53Veh Mid
SA019977B_58Veh Mid
SA019978B_56Veh Mid
SA019979B_16Veh OlF
SA019980B_20Veh OlF
SA019981B_7Veh OlF
SA019982B_4Veh OlF
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Collection:

Collection ID:CO000423
Collection Summary:24 hr after the social test, mice were deeply anesthetized with 5% isoflurane, followed by rapid decapitation. Brains were removed, rinsed with ice-cold water, and then rapidly dissected into the following 5 regions: forebrain, midbrain, cerebellum, hindbrain, and olfactory bulbs. The dissected parts were flash-frozen on dry ice and stored at -80o C. Samples were coded by treatment: vehicle-treated mice (n=8) and OT-treated mice (n=12).
Sample Type:Brain

Treatment:

Treatment ID:TR000443
Treatment Summary:BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection.

Sample Preparation:

Sampleprep ID:SP000436
Sampleprep Summary:Each thawed tissue sample was transferred into a pre-labeled MagnaLyser tube and massed. 50% acetonitrile in water was added depending on the mass of each sample (500 uL for up to 200 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. A volume of each sample supernatant required to analyze the desired sample mass (20 mg for Mid, Cerb, Hind, Fore, and 10 mg for Olf) was aliquoted. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume of extracted tissue. Five replicates of the total study pool were created and prepared identically to the study samples. Samples were frozen and lyophilized. Lyophilized samples were mixed with 250 μl of Master mix (0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6 and 6mM Imidazole). The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

Analysis ID:AN000648
Analysis Type:NMR
Num Factors:11

NMR:

NMR ID:NM000070
Analysis ID:AN000648
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:700 MHz
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