Summary of Study ST000410

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000410
Study TitleMetabolomic analysis of oxytocin effects on social deficits in mice (part III)
Study SummaryThe goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in brain.
Institute
University of North Carolina
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-17
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailNMR
Release Date2017-07-10
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8MP4W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000320
Project DOI:doi: 10.21228/M8MP4W
Project Title:Metabolomic analysis of oxytocin effects on social deficits in mice
Project Summary:The goal of this study was to determine the effects of the neuropeptide oxytocin (OT) on metabolomic profiles, using a mouse model of autism-like behavior, the BALB/cByJ inbred strain. We have previously reported that subchronic treatment with OT can lead to persistent reversal of social deficits in BALB/cByJ and other models of autism spectrum disorder (ASD). In this study, mice were given a subchronic regimen with either vehicle or OT, and then evaluated for social approach. At the end of the study, brain and blood were collected for metabolomic analysis. In addition, fecal samples were taken at different time points during the treatment and testing regimen. The results from this project could elucidate mechanisms underlying the prosocial effects of oxytocin, and identify new targets for the development of highly specific oxytocin-related drugs.
Institute:University of North Carolina at Chapel Hill
Department:Department of Psychiatry
Last Name:Moy
First Name:Sheryl
Address:CB# 7146, Chapel Hill, NC 277599
Email:ssmoy@med.unc.edu
Phone:919-966-3082

Subject:

Subject ID:SU000431
Subject Type:Mice
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mice; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Brain region
SA020072B_68Oxytocin Cerb
SA020073B_70Oxytocin Cerb
SA020074B_66Oxytocin Cerb
SA020075B_65Oxytocin Cerb
SA020076B_62Oxytocin Cerb
SA020077B_72Oxytocin Cerb
SA020078B_61Oxytocin Cerb
SA020079B_63Oxytocin Cerb
SA020080B_77Oxytocin Cerb
SA020081B_79Oxytocin Cerb
SA020082B_75Oxytocin Cerb
SA020083B_74Oxytocin Cerb
SA020084B_30Oxytocin Fore
SA020085B_34Oxytocin Fore
SA020086B_37Oxytocin Fore
SA020087B_39Oxytocin Fore
SA020088B_28Oxytocin Fore
SA020089B_35Oxytocin Fore
SA020090B_32Oxytocin Fore
SA020091B_23Oxytocin Fore
SA020092B_21Oxytocin Fore
SA020093B_25Oxytocin Fore
SA020094B_22Oxytocin Fore
SA020095B_26Oxytocin Fore
SA020096B_97Oxytocin Hind
SA020097B_99Oxytocin Hind
SA020098B_85Oxytocin Hind
SA020099B_94Oxytocin Hind
SA020100B_95Oxytocin Hind
SA020101B_86Oxytocin Hind
SA020102B_90Oxytocin Hind
SA020103B_82Oxytocin Hind
SA020104B_88Oxytocin Hind
SA020105B_81Oxytocin Hind
SA020106B_83Oxytocin Hind
SA020107B_92Oxytocin Hind
SA020108B_50Oxytocin Mid
SA020109B_57Oxytocin Mid
SA020110B_59Oxytocin Mid
SA020111B_55Oxytocin Mid
SA020112B_54Oxytocin Mid
SA020113B_52Oxytocin Mid
SA020114B_46Oxytocin Mid
SA020115B_42Oxytocin Mid
SA020116B_43Oxytocin Mid
SA020117B_41Oxytocin Mid
SA020118B_45Oxytocin Mid
SA020119B_48Oxytocin Mid
SA020120B_3Oxytocin OlF
SA020121B_15Oxytocin OlF
SA020122B_14Oxytocin OlF
SA020123B_19Oxytocin OlF
SA020124B_6Oxytocin OlF
SA020125B_5Oxytocin OlF
SA020126B_8Oxytocin OlF
SA020127B_10Oxytocin OlF
SA020128B_1Oxytocin OlF
SA020129B_12Oxytocin OlF
SA020130B_2Oxytocin OlF
SA020131PB_3Pool Pool
SA020132PB_2Pool Pool
SA020133PB_4Pool Pool
SA020134PB_1Pool Pool
SA020135B_73Veh Cerb
SA020136B_71Veh Cerb
SA020137B_78Veh Cerb
SA020138B_80Veh Cerb
SA020139B_64Veh Cerb
SA020140B_76Veh Cerb
SA020141B_67Veh Cerb
SA020142B_69Veh Cerb
SA020143B_24Veh Fore
SA020144B_38Veh Fore
SA020145B_40Veh Fore
SA020146B_27Veh Fore
SA020147B_36Veh Fore
SA020148B_29Veh Fore
SA020149B_33Veh Fore
SA020150B_31Veh Fore
SA020151B_87Veh Hind
SA020152B_89Veh Hind
SA020153B_96Veh Hind
SA020154B_100Veh Hind
SA020155B_98Veh Hind
SA020156B_93Veh Hind
SA020157B_91Veh Hind
SA020158B_84Veh Hind
SA020159B_56Veh Mid
SA020160B_51Veh Mid
SA020161B_49Veh Mid
SA020162B_44Veh Mid
SA020163B_53Veh Mid
SA020164B_47Veh Mid
SA020165B_58Veh Mid
SA020166B_60Veh Mid
SA020167B_16Veh OlF
SA020168B_11Veh OlF
SA020169B_18Veh OlF
SA020170B_13Veh OlF
SA020171B_4Veh OlF
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Collection:

Collection ID:CO000425
Collection Summary:24 hr after the social test, mice were deeply anesthetized with 5% isoflurane, followed by rapid decapitation. Brains were removed, rinsed with ice-cold water, and then rapidly dissected into the following 5 regions: forebrain, midbrain, cerebellum, hindbrain, and olfactory bulbs. The dissected parts were flash-frozen on dry ice and stored at -80o C. Samples were coded by treatment: vehicle-treated mice (n=8) and OT-treated mice (n=12).
Sample Type:Brain

Treatment:

Treatment ID:TR000445
Treatment Summary:BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection.

Sample Preparation:

Sampleprep ID:SP000438
Sampleprep Summary:The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.

Analysis:

Analysis ID:AN000650
Analysis Type:NMR
Num Factors:11
Num Metabolites:14
Units:ng

NMR:

NMR ID:NM000072
Analysis ID:AN000650
Instrument Name:Thermo Scientific 5600A 16 channel coulometric array ECD
Instrument Type:Other
NMR Experiment Type:Other
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