Summary of Study ST000418

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000327. The data can be accessed directly via it's Project DOI: 10.21228/M8GS39 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000418
Study TitleThe role of CFTR in the regulation of intrinsic defense mechanisms of exocrine secretions
Study SummaryThis study was designed as a pilot to determine if the concentrations of selected ions in salivary gland secretions were influenced by the absence of CFTR on the apical surfaces of glandular cells and ductal epithelia. To this end, five cystic fibrosis children homozygous for Phe508 were to be recruited with their heterozygous non-CF mothers. For adaptation and development of the assays to a microtiter format, saliva samples were collected from non-CF control subjects initially to optimize the assays recognizing that the volumes for analyses would be small. The values obtained with these non-CF control samples were also compared to that of the homozygous and heterozygous CF subjects. As residual volume permits, these samples will be further analyzed for metabolic constituent profiles.
Institute
University of North Carolina
DepartmentRCMRC
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-15
Num Groups3
Total Subjects22
Num Males10
Num Females12
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2018-06-05
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8GS39
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000327
Project DOI:doi: 10.21228/M8GS39
Project Title:The role of CFTR in the regulation of intrinsic defense mechanisms of exocrine secretions
Project Summary:This study was designed as a pilot to determine if the concentrations of selected ions in salivary gland secretions were influenced by the absence of CFTR on the apical surfaces of glandular cells and ductal epithelia. To this end, five cystic fibrosis children homozygous for Phe508 were to be recruited with their heterozygous non-CF mothers. Unstimulated glandular secretions to include parotid and submandibular/sublingual saliva samples as well as whole, mixed saliva collected with masticatory stimulation were to be collected from the child and the mother and analyzed for nitrate (NO3), nitrite (NO2), bicarbonate (HCO3), thiocyanate (SCN), reduced and oxidized glutathione (GSH and GSSG) and buffering capacity (pH before and after addition of HCl to 3.75mN). For adaptation and development of the assays to a microtiter format, saliva samples were collected from non-CF control subjects initially to optimize the assays recognizing that the volumes for analyses would be small. The values obtained with these non-CF control samples were also compared to that of the homozygous and heterozygous CF subjects. As residual volume permits, these samples will be further analyzed for metabolic constituent profiles.
Institute:University of North Carolina at Chapel Hill
Department:School of Dentistry
Last Name:Arnold
First Name:Roland
Address:385 S Columbia Street, Chapel Hill, NC 27599
Email:roland_arnold@unc.edu
Phone:919-537-3165

Subject:

Subject ID:SU000439
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male/Female
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA021013S_14CF
SA021014S_16CF
SA021015S_17CF
SA021016S_18CF
SA021017S_15CF
SA021018S_19MOM
SA021019S_22MOM
SA021020S_20MOM
SA021021S_21MOM
SA021022S_23MOM
SA021023S_4NCF
SA021024S_11NCF
SA021025S_3NCF
SA021026S_1NCF
SA021027S_5NCF
SA021028S_9NCF
SA021029S_8NCF
SA021030S_2NCF
SA021031S_7NCF
SA021032S_12NCF
SA021033S_13NCF
SA021034S_6NCF
Showing results 1 to 22 of 22

Collection:

Collection ID:CO000433
Collection Summary:Human saliva was collected from patients with Cystic Fibrosis along with their heterozygous non-CF mothers,and non-CF control subjects.
Sample Type:Saliva

Treatment:

Treatment ID:TR000453
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000446
Sampleprep Summary:Aliquots of 150 µL human saliva were mixed with 0.600 mL of degassed 3:3:2 Acetonitrile:Isopropanol:Water solution by vortex mixer then centrifuged. Supernatant recovered and evaporated to dryness on speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Combined analysis:

Analysis ID AN000660
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus 4D GC
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH000477
Instrument Name:Leco Pegasus 4D GC
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Pressure:12.8psi
Flow Gradient:constant flow
Flow Rate:1ml/min
Injection Temperature:250C
Retention Index:FAME markers C8-C30
Chromatography Type:GC

MS:

MS ID:MS000586
Analysis ID:AN000660
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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