Summary of Study ST000443

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000330. The data can be accessed directly via it's Project DOI: 10.21228/M8W02Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000443
Study TitleQuantitative measurements of TCA cycle metabolites in T1D poor control, good control, and controls.
Study TypeQuantitative measurements of plasma levels of tricarboxylic acid (TCA) cycle metabolites
Study SummaryThe objective of the study was to determine whether T1D with good glycemic control have persistent abnormalities of metabolites and pathways that exist in T1D with poor glycemic control.
Institute
Mayo Clinic
DepartmentEndocrinology
LaboratoryMayo Clinic Metabolomics Resource Core
Last NameNair
First NameSreekumaran
Address200 First Street SW, Rochester, MN 55905
EmailNair.K@mayo.edu
Phone507-285-2415
Submit Date2016-07-20
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2016-09-23
Release Version1
Sreekumaran Nair Sreekumaran Nair
https://dx.doi.org/10.21228/M8W02Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000330
Project DOI:doi: 10.21228/M8W02Q
Project Title:Impact of Long-Term Poor and Good Glycemic Control on Metabolomics Alterations in Type 1 Diabetic People.
Project Type:Untargeted LC-MS Metabolomics
Project Summary:The objective of the study was to determine whether T1D with good glycemic control have persistent abnormalities of metabolites and pathways that exist in T1D with poor glycemic control.
Institute:Mayo Clinic
Department:Endocrinology
Laboratory:Mayo Clinic Metabolomics Resource Core
Last Name:Nair
First Name:Sreekumaran
Address:200 First Street SW, Rochester, MN 55905
Email:Nair.K@mayo.edu
Phone:507-285-2415

Subject:

Subject ID:SU000464
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id treatment
SA022562sample33ND
SA022563sample34ND
SA022564sample35ND
SA022565sample32ND
SA022566sample28ND
SA022567sample24ND
SA022568sample26ND
SA022569sample36ND
SA022570sample31ND
SA022571sample37ND
SA022572sample42ND
SA022573sample43ND
SA022574sample44ND
SA022575sample41ND
SA022576sample40ND
SA022577sample38ND
SA022578sample39ND
SA022579sample22ND
SA022580sample30ND
SA022581sample16ND
SA022582sample14ND
SA022583sample10ND
SA022584sample12ND
SA022585sample8ND
SA022586sample6ND
SA022587sample2ND
SA022588sample20ND
SA022589sample18ND
SA022590sample4ND
SA022591sample11T1D good glycemic control
SA022592sample21T1D good glycemic control
SA022593sample3T1D good glycemic control
SA022594sample9T1D good glycemic control
SA022595sample5T1D good glycemic control
SA022596sample7T1D good glycemic control
SA022597sample25T1D good glycemic control
SA022598sample23T1D good glycemic control
SA022599sample13T1D good glycemic control
SA022600sample27T1D good glycemic control
SA022601sample19T1D good glycemic control
SA022602sample15T1D good glycemic control
SA022603sample17T1D good glycemic control
SA022604sample1T1D good glycemic control
SA022605sample29T1D good glycemic control
SA022606sample55T1D poor glycemic control
SA022607sample54T1D poor glycemic control
SA022608sample56T1D poor glycemic control
SA022609sample58T1D poor glycemic control
SA022610sample53T1D poor glycemic control
SA022611sample57T1D poor glycemic control
SA022612sample48T1D poor glycemic control
SA022613sample46T1D poor glycemic control
SA022614sample45T1D poor glycemic control
SA022615sample47T1D poor glycemic control
SA022616sample49T1D poor glycemic control
SA022617sample51T1D poor glycemic control
SA022618sample50T1D poor glycemic control
SA022619sample52T1D poor glycemic control
Showing results 1 to 58 of 58

Collection:

Collection ID:CO000458
Collection Summary:At 5:00 AM after an overnight fast, baseline blood samples were collected from study participants. Plasma samples were stored at 80°C until analysis.
Sample Type:Blood. Plasma was isolated for MS analysis.

Treatment:

Treatment ID:TR000478
Treatment Summary:Participants were admitted to the Clinical Research Unit at St Mary’s Hospital (Rochester, Minnesota) the evening before the study and spent overnight in the Clinical Research Unit. The participants were given a standard meal on the evening of the admission after which they fasted overnight. Participants with T1D were treated with insulin as per their usual individual programs. At 5:00 AM after an overnight fast, baseline blood samples were collected from study participants. Plasma samples were stored at 80°C until analysis.

Sample Preparation:

Sampleprep ID:SP000471
Sampleprep Summary:Plasma quality-control samples used in the study were prepared from pooled plasma spiked with a selection of metabolites to mimic elevated levels of metabolites during I− (insulin withdrawn) condition. Plasma was spiked with a standard mixture (3:1 ratio of plasma to spiking solution) containing 100 μg/mL niacin, hypoxanthine, leucine, isoleucine, phenylalanine, tryptophan, citric acid, glucose, hippuric acid, and taurocholic acid dissolved in 1:1 acetonitrile/water. All plasma samples (200 μL) were thawed on ice at 4°C followed by deproteinization with methanol (1:4 ratio of plasma to methanol) and vortexed for 10 s, followed by incubation at −20°C for 2 h. The samples were then centrifuged at 15,871g for 30 min at 4°C. The supernatants were lyophilized (Savant, Holbrook, NY) and stored at −20°C prior to analysis. The samples were reconstituted in 50% H2O/acetonitrile and passed through a Microcon YM3 filter (Millipore Corporation). The supernatants were transferred to analytical vials, stored in the autosampler at 4°C, and analyzed within 48 h of reconstitution in buffer.

Combined analysis:

Analysis ID AN000694
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890
Column None
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5973
Ion Mode POSITIVE
Units micromolar

Chromatography:

Chromatography ID:CH000502
Chromatography Summary:The derivatized extracts were analyzed with an Agilent 6890 gas chromatograph coupled with an Agilent 5973 mass selective detector. The 1-μL aliquots of the extracts were injected into a DB5-MS capillary column (30 m × 250 μm i.d., 0.25-μm film thickness; J&W Scientific, Folson, CA) using PTV injection (Gerstel CIS4 injector) in the splitless mode. The temperature of the PTV was 70 °C during injection, and 0.6 min after injection, the temperature was raised to 300 °C at a rate of 2 °C/s and held at 300 °C for 20 min. The initial GC oven temperature was 70 °C, 5 min after injection the GC oven temperature was increased with 5 °C/min to 320 °C and held for 5 min at 320 °C. Helium was used as a carrier gas and pressure programmed such that the helium flow was kept constant at a flow rate of 1.7 mL/min. Detection was achieved using MS detection in electron impact mode and full scan monitoring mode (m/z 15−800). The temperature of the ion source was set at 250 °C and that of the quadrupole at 200 °C.
Instrument Name:Agilent 6890
Column Name:None
Chromatography Type:GC

MS:

MS ID:MS000616
Analysis ID:AN000694
Instrument Name:Agilent 5973
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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