Summary of study ST000446

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000344. The data can be accessed directly via it's Project DOI: 10.21228/M8KK69 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download all metabolite data  |  Perform analysis on untargeted data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data
Study IDST000446
Study TitleHeterologous expression and detection of Apratoxins in E. coli
Study TypeOrganic extraction of E coli cultures harboring apratoxin gene cluster
Study SummaryThe apratoxin gene cluster was recovered from fosmid DNA library. The gene set responsible for the biosynthesis of the polyketide backbone was introduced in E. coli BAP strain and expressed at 30C for 24 hours. Two sets of control and experimental samples with culture broth pH adjusted at 8 and 12 respectively was extracted with ethyl acetate and dried.
Institute
University of Florida
DepartmentSECIM
Last NameKallifidas
First NameDimitris
AddressMedical Sciences Building, Rm P5-26, 1600 SW Archer Rd., FL32610
Emaildkallifidas@ufl.edu
PhoneNone
Submit Date2016-07-28
Num Groups1
Total Subjects12
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Dimitris Kallifidas Dimitris Kallifidas
https://dx.doi.org/10.21228/M8KK69
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000344
Project DOI:doi: 10.21228/M8KK69
Project Title:Cloning, expression and derivatization of cytotoxic marine natural product Apratoxin
Project Summary:Apratoxin is a hybrid PKS/NRPS secondary metabolite showing potent anticancer activity by inducing G1 phase cell cycle arrest and apoptosis. There have been reported a series of natural apratoxin derivatives with defined chemical modifications in their structures that alter their biological activity. Looking for a better activity, total synthesis of apratoxin has been achieved and a series of synthetic congeners showed increased activity with subnanomolar potency. From the total synthesis point of view, the modification of the polyketide backbone presents a burden for a radical derivatization of apratoxins. We hypothesized that supply the polyketide part naturally by overexpression the corresponding genes it will facilitate the generation of apratoxin congeners by decorating the backbone with a variety of amino acids improving the antitumor activity.Threfore, the apratoxin gene cluster was recovered from a fosmid DNA library. The gene set responsible for the biosynthesis of the polyketide backbone was introduced in E. coli BAP strain and expressed at 30C for 24 hours. Two sets of control and experimental samples with culture broth pH adjusted at 8 and 12 respectively was extracted with ethyl acetate and dried.
Institute:University of Florida
Department:Medicinal Chemistry
Last Name:Luesch
First Name:Hendrik
Address:Medical Sciences Building, Rm P5-26, 1600 SW Archer Rd., FL32610
Email:luesch@cop.ufl.edu
Phone:none
  logo