Summary of Study ST000449

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000347. The data can be accessed directly via it's Project DOI: 10.21228/M8689X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000449
Study TitleMolecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry
Study SummaryThis study investigates the metabolism and antibiotic inhibition in Escherichia coli and Bacillus subtilis colonies.
Institute
George Washington University
DepartmentChemistry
LaboratoryVertes Research Lab
Last NameLi
First NameHang
Address800 22nd St. NW, Suite 4000, Washington, DC 20052
Emailamy_li@gwmail.gwu.edu
Phone202-994-6344
Submit Date2016-08-09
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Hang Li Hang Li
https://dx.doi.org/10.21228/M8689X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000347
Project DOI:doi: 10.21228/M8689X
Project Title:Molecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry
Project Summary:This study utilized laser ablation electrospray ionization mass spectrometry imaging to study the metabolism and antibiotic inhibition in bacterial colonies.
Institute:The George Washington University
Department:Chemistry
Laboratory:Vertes Research Lab
Last Name:Li
First Name:Hang
Address:800 22nd St. NW, Suite 4000, Washington, DC 20052
Email:amy_li@gwmail.gwu.edu
Phone:202-994-6344

Subject:

Subject ID:SU000470
Subject Type:Microbial (Escherichia coli, bacillus subtilis)
Subject Species:Bacillus subtilis
Taxonomy ID:1423
Genotype Strain:ATCC12435, ATCC6051
Species Group:Microorganism

Factors:

Subject type: Microbial (Escherichia coli, bacillus subtilis); Subject species: Bacillus subtilis (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA022780EC-1Blank
SA022781BS-1Blank
SA022782ECBS-1Streptomycin
SA022783BS-2Streptomycin-penicillin
Showing results 1 to 4 of 4

Collection:

Collection ID:CO000464
Collection Summary:All the sample were analyzed using laser ablation electrospray ionization (LAESI) MS or MSI.
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR000484
Treatment Summary:In the antibiotic susceptibility testing, the bacterial colonies were treated with streptomycin or streptomycin-penicillin.

Sample Preparation:

Sampleprep ID:SP000477
Sampleprep Summary:The E. coli and B. subtilis colonies were inoculated and incubated following the standard protocol. The antibiotic inhibition tests were performed according to the conventional Kirby-Bauer antibiotic testing.

Combined analysis:

Analysis ID AN000703 AN000704
Analysis type MS MS
Chromatography type None (Direct infusion) None (Direct infusion)
Chromatography system Synapt G2S Synapt G2S
Column None None
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 Waters Synapt G2
Ion Mode NEGATIVE POSITIVE
Units m/z m/z

Chromatography:

Chromatography ID:CH000509
Chromatography Summary:No chromatography was used for this study
Instrument Name:Synapt G2S
Column Name:None
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS000625
Analysis ID:AN000703
Instrument Name:Waters Synapt G2
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000626
Analysis ID:AN000704
Instrument Name:Waters Synapt G2
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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