Summary of Study ST000461

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.


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Study IDST000461
Study TitleEffect of Odc knockout on amino acid homeostasis of Eu-Myc Lymphomas
Study TypeLymphomas isolated from Eu-Myc;CD19-Cre;Odc fl/fl mice
Study SummaryAmino acid levels analysis of lymphomas isolated from Eu-Myc;CD19-Cre;Odc fl/fl mic and their wild-type counterparts Eu-Myc;CD19-Cre;Odc +/+
University of Florida
LaboratoryJohn Cleveland
Last NameFernandez
First NameMario
Address12902 Magnolia Drive, MRC 4-West
Submit Date2016-09-08
Num Groups4
Total Subjects12
Study CommentsLymphomas isolated from Eu-Myc;CD19-Cre;Odc fl/fl mice in triplicate
Analysis Type DetailLC-MS
Release Date2020-07-08
Release Version1
Mario Fernandez Mario Fernandez application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000354
Project DOI:doi: 10.21228/M8930Q
Project Title:The effect of BCAT1 knockdown on amino acid homeostasis
Project Type:Amino acid steady-state level analysis
Project Summary:Raji cells, a Burkitt lymphoma, expressing a Dox-inducible short hairpin targeting BCAT1 were collected in triplicate at 0, 48, 96, and 144 hours.
Institute:H. Lee Moffitt Cancer Center
Department:Tumor Biology
Laboratory:John Cleveland
Last Name:Fernandez
First Name:Mario
Address:12902 Magnolia Drive, MRC 4-West
Funding Source:5R01CA154739


Subject ID:SU000482
Subject Type:Cells
Subject Species:Mus musculus
Taxonomy ID:10090
Cell Strain Details:BIT
Subject Comments:See study design section for cell number per sample
Cell Primary Immortalized:Immortalized
Species Group:Mammal


Subject type: Cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA023262BIT_012Odc -/-
SA023263BIT_011Odc -/-
SA023264BIT_007Odc -/-
SA023265BIT_008Odc -/-
SA023266BIT_009Odc -/-
SA023267BIT_010Odc -/-
SA023256BIT_001Odc +/+
SA023257BIT_006Odc +/+
SA023258BIT_005Odc +/+
SA023259BIT_002Odc +/+
SA023260BIT_003Odc +/+
SA023261BIT_004Odc +/+
Showing results 1 to 12 of 12


Collection ID:CO000476
Collection Summary:Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C.
Sample Type:Cell
Collection Method:Pellet
Collection Location:Lab
Collection Time:Collected during log phase
Storage Conditions:Pellet at -80C
Storage Vials:1.5 mL microfuge tube
Tissue Cell Identification:BIT


Treatment ID:TR000496
Treatment Summary:Cells were not treated. Cells were collected during log phase
Cell Growth Container:6-well plate

Sample Preparation:

Sampleprep ID:SP000489
Sampleprep Summary:Amino acid
Sampleprep Protocol Filename:SB_AA_Assay.pdf

Combined analysis:

Analysis ID AN000722
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um)
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Units nmol/mg protein


Chromatography ID:CH000519
Chromatography Summary:Amino Acid
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase


MS ID:MS000642
Analysis ID:AN000722
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Comments:Amino Acids