Summary of Study ST000463

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000355. The data can be accessed directly via it's Project DOI: 10.21228/M85C88 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000463
Study TitleCNS and peripheral metabolomics of calorie restriction in a mouse model of Alzheimer’s disease (part II)
Study SummaryThis pilot metabolomic study will evaluate cecal specimens from an established mouse model of AD, the tq2576 mouse model of cerebral amyloid overexpression, in comparison to their non-transgenic (ntg) littermates. These animals were either on a CR or ad libitum (AL) diet, and specimens were collected at two time points (5 and 15 months of age). Tissue from this cohorts of mice have already undergone microbiome analysis, and await coordinated brain and peripheral tissue assessments. Future analysis will include metabolomics, RNA-seq, and microarray data to assess the gut-brain microbiome system in neurodegenerative disorders.
University of North Carolina
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Submit Date2016-09-07
Raw Data AvailableYes
Raw Data File Type(s)1r
Analysis Type DetailNMR
Release Date2017-10-03
Release Version1
Susan Sumner Susan Sumner application/zip

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Project ID:PR000355
Project DOI:doi: 10.21228/M85C88
Project Title:CNS and peripheral metabolomics of calorie restriction in a mouse model of Alzheimer’s disease
Project Summary:Alzheimer’s disease (AD) is a devastating neurodegenerative disorder that robs people of their memory and cognitive function. Currently, no successful treatment or preventative measure exists for AD. Calorie restriction (CR) is a dietary regimen posited to suppress genetic programs of aging and reduce AD-related pathology. CR is known to enhance longevity and mitigate aging phenotypes in multiple model species. Mechanisms underlying the benefits of CR remain unknown, particularly in areas of the brain selectively vulnerable to age-related AD pathology such as the hippocampus, a region crucial for learning and memory. Moreover, AD pathology can be influenced by changes in diet, metabolism, and immunity, indicating that factors distant from the brain may play a role in pathogenesis. The intestinal microbiota, composed of trillions of microbial cells, influences host metabolism, immunity, and cognitive function, and is posited to be linked mechanistically to AD pathobiology, but a specific role remains to be adequately tested. We hypothesize that mechanisms underlying the benefits of CR are cell-type and organ specific, involving the gut-brain microbiome throughout the lifespan, this requiring subregional analysis in the brain as well as coordinated assessments of key peripheral targets including the liver, fecal pellets , and plasma. Thus, CR is proposed to be a viable treatment option that may ameliorate the development of AD-related pathology, and importantly, reveal mechanisms that attenuate age-related expression changes in vulnerable cells.
Institute:New York University
Department: Langone Medical Center
Last Name:Ginsberg
First Name:Stephen
Address:140 Old Orangeburg Road, Orangeburg, NY 10962


Subject ID:SU000484
Subject Type:Murine
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal


Subject type: Murine; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age Genotype Gender Diet
SA023346C_1015 APP F AL
SA023347C_1115 APP F AL
SA023348C_915 APP F AL
SA023349C_1215 APP F AL
SA023350C_1615 APP F CR
SA023351C_1515 APP F CR
SA023352C_1415 APP F CR
SA023353C_1315 APP F CR
SA023354C_4415 APP M AL
SA023355C_4315 APP M AL
SA023356C_4215 APP M AL
SA023357C_4615 APP M AL
SA023358C_4515 APP M AL
SA023359C_5015 APP M CR
SA023360C_4915 APP M CR
SA023361C_4815 APP M CR
SA023362C_7715 APP M CR
SA023363C_4715 APP M CR
SA023364C_2515 NTG F AL
SA023365C_2715 NTG F AL
SA023366C_2415 NTG F AL
SA023367C_2615 NTG F AL
SA023368C_2915 NTG F CR
SA023369C_3015 NTG F CR
SA023370C_3115 NTG F CR
SA023371C_2815 NTG F CR
SA023372C_6115 NTG M AL
SA023373C_6415 NTG M AL
SA023374C_6215 NTG M AL
SA023375C_6315 NTG M AL
SA023376C_6515 NTG M AL
SA023377C_6815 NTG M CR
SA023378C_6915 NTG M CR
SA023379C_6615 NTG M CR
SA023380C_7015 NTG M CR
SA023381C_6715 NTG M CR
SA023382C_45 APP F AL
SA023383C_35 APP F AL
SA023384C_15 APP F AL
SA023385C_25 APP F AL
SA023386C_85 APP F CR
SA023387C_75 APP F CR
SA023388C_65 APP F CR
SA023389C_55 APP F CR
SA023390C_335 APP M AL
SA023391C_345 APP M AL
SA023392C_365 APP M AL
SA023393C_325 APP M AL
SA023394C_355 APP M AL
SA023395C_395 APP M CR
SA023396C_415 APP M CR
SA023397C_375 APP M CR
SA023398C_405 APP M CR
SA023399C_385 APP M CR
SA023400C_195 NTG F AL
SA023401C_185 NTG F AL
SA023402C_175 NTG F AL
SA023403C_215 NTG F CR
SA023404C_205 NTG F CR
SA023405C_225 NTG F CR
SA023406C_235 NTG F CR
SA023407C_535 NTG M AL
SA023408C_545 NTG M AL
SA023409C_515 NTG M AL
SA023410C_555 NTG M AL
SA023411C_525 NTG M AL
SA023412C_565 NTG M CR
SA023413C_575 NTG M CR
SA023414C_585 NTG M CR
SA023415C_605 NTG M CR
SA023416C_595 NTG M CR
SA023417CP_3Pool Pool Pool Pool
SA023418CP_2Pool Pool Pool Pool
SA023419CP_4Pool Pool Pool Pool
SA023420CP_6Pool Pool Pool Pool
SA023421CP_1Pool Pool Pool Pool
SA023422CP_5Pool Pool Pool Pool
Showing results 1 to 77 of 77


Collection ID:CO000478
Collection Summary:Cecal pellets
Sample Type:Brain


Treatment ID:TR000498
Treatment Summary:Calorie restricted (CR) and ad libitum (AL) diets

Sample Preparation:

Sampleprep ID:SP000491
Sampleprep Summary:Cecal samples were homogenized with HPLC grade water, and the final concentration of all of the extracts was 0.5 mg/µL. All 70 samples were included in an analytical quality control (QC) total pool. This total pool was created with 50 µL of each sample, which were divided into six replicates. A 250 µL aliquot of each sample and pool homogenate was filtered, and 225 µL of the filtrate was lyophilized. Lyophilized samples were reconstituted with 700 µL of Phosphate buffer with 0.5 mM DSS. Samples were vortexed and then centrifuged before transferring 600 µL of each sample into pre-labeled 5mm NMR tubes for data acquisition on a 700 MHz spectrometer.


Analysis ID:AN000724
Analysis Type:NMR
Num Factors:17


NMR ID:NM000081
Analysis ID:AN000724
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:700 MHz