Summary of Study ST000474
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000362. The data can be accessed directly via it's Project DOI: 10.21228/M8831S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000474 |
| Study Title | Metabolomic testing of a putative MDH inhibitor (targeted) |
| Study Type | drug treatment (single dose and timepoint) |
| Study Summary | HL-60 cells will be treated for 15hr with a putative MDH inhibitor or vehicle (n=5 per group) |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Case Western University |
| Last Name | Wald |
| First Name | David |
| Address | 2103 Cornell Rd WRB3-530 Cleveland, OH 44106 |
| dnw@case.edu | |
| Phone | 2163685668 |
| Submit Date | 2016-08-31 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Study Comments | Total subjects: 10 for global testing and 10 for targeted testing |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000362 |
| Project DOI: | doi: 10.21228/M8831S |
| Project Title: | Metabolomic testing of a putative MDH inhibitor |
| Project Summary: | Testing for metabolomic changes after treatment of leukemia cells with a putative MDH inhibitor |
| Institute: | Case Western Reserve University |
| Department: | Pathology |
| Laboratory: | Foundation and Institutional funds |
| Last Name: | Wald |
| First Name: | David |
| Address: | 2103 Cornell Rd WRB3-530 Cleveland, OH 44106 |
| Email: | dnw@case.edu |
| Phone: | NA |
Subject:
| Subject ID: | SU000495 |
| Subject Type: | Human leukemia cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | HL-60 |
| Subject Comments: | immortalized |
| Cell Counts: | 10 million per sample |
| Species Group: | Mammals |
Factors:
Subject type: Human leukemia cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA024328 | ut1 | - |
| SA024329 | ut5 | - |
| SA024330 | ut4 | - |
| SA024331 | ut2 | - |
| SA024332 | ut3 | - |
| SA024333 | tx5 | MDH inhib |
| SA024334 | tx4 | MDH inhib |
| SA024335 | tx1 | MDH inhib |
| SA024336 | tx2 | MDH inhib |
| SA024337 | tx3 | MDH inhib |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000489 |
| Collection Summary: | Cells were centrifuged to remove growth media/drug and either flash frozen or washed 1x in PBS. The cells were washed for the targeted assay but not for the global assay. |
| Sample Type: | cell |
| Storage Conditions: | flash frozen |
| Tissue Cell Quantity Taken: | 10 million per sample |
Treatment:
| Treatment ID: | TR000509 |
| Treatment Summary: | Cells were treated for 15hr with 1 micromolar of drug or vehicle |
| Cell Growth Container: | flasks |
| Cell Growth Rate: | ~24hr per division |
| Cell Media: | RPMI with 10% FBS |
| Cell Envir Cond: | 5% CO2 incubator |
| Cell Media Lastchanged: | 15 hr |
Sample Preparation:
| Sampleprep ID: | SP000502 |
| Sampleprep Summary: | Amino Acid|Global |
| Sampleprep Protocol Filename: | SB_OA_Assay.pdf Appendix_C_Protein_DNA_quant.pdf |
| Sample Spiking: | Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
| Analysis ID | AN000738 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE |
| Units | Total nmoles (with relative protein adjustment) |
Chromatography:
| Chromatography ID: | CH000531 |
| Chromatography Summary: | Amino acids |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000655 |
| Analysis ID: | AN000738 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
