Summary of Study ST000478

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000364. The data can be accessed directly via it's Project DOI: 10.21228/M80K61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000478
Study Title	NMR based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation
Study Summary	Tissue extracts from ionizing readiation exposed mouse liver and controls were compared via NMR based metabolomic analysis
Institute	Pacific Northwest National Laboratory
Department	Fundamental & Computational Sciences
Last Name	Hu
First Name	Jianzhi
Address	Pacific Northwest National Laboratory, Richland, WA 99352
Email	Jianzhi.Hu@pnnl.gov
Phone	+1 (509) 371-6544
Submit Date	2016-08-11
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2017-07-10
Release Version	1

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Project:

Project ID:	PR000364
Project DOI:	doi: 10.21228/M80K61
Project Title:	NMR based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation
Project Summary:	Tissue extracts from ionizing readiation exposed mouse liver and controls were compared via NMR based metabolomic analysis
Institute:	Pacific Northwest National Laboratory
Department:	Fundamental & Computational Sciences
Last Name:	Hu
First Name:	Jianzhi
Address:	Pacific Northwest National Laboratory, Richland, WA 99352
Email:	Jianzhi.Hu@pnnl.gov
Phone:	+1 (509) 371-6544

Subject:

Subject ID:	SU000499
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	days post exposure to radiation	radiation dose	radiation source
SA024487	G315	11	3.0	gamma
SA024488	G311	11	3.0	gamma
SA024489	G314	11	3.0	gamma
SA024490	G313	11	3.0	gamma
SA024491	G312	11	3.0	gamma
SA024492	P311	11	3.0	proton
SA024493	P312	11	3.0	proton
SA024494	P315	11	3.0	proton
SA024495	P314	11	3.0	proton
SA024496	P313	11	3.0	proton
SA024483	G013	11	-	sham
SA024484	G014	11	-	sham
SA024485	G012	11	-	sham
SA024486	G011	11	-	sham
SA024501	G344	4	3.0	gamma
SA024502	G342	4	3.0	gamma
SA024503	G343	4	3.0	gamma
SA024504	G345	4	3.0	gamma
SA024505	G341	4	3.0	gamma
SA024506	G743	4	7.8	gamma
SA024507	G744	4	7.8	gamma
SA024508	G742	4	7.8	gamma
SA024509	G741	4	7.8	gamma
SA024497	G042	4	-	sham
SA024498	G043	4	-	sham
SA024499	G044	4	-	sham
SA024500	G041	4	-	sham

Showing results 1 to 27 of 27

Showing results 1 to 27 of 27

Collection:

Collection ID:	CO000493
Collection Summary:	Livers were dissected and snap-frozen at -80°C before NMR spectroscopy and morphology analysis.
Sample Type:	Liver

Treatment:

Treatment ID:	TR000513
Treatment Summary:	A total of 27 seven-week-old C57BL/6 female mice randomly into 6 groups, and then whole body being exposed to either gamma or proton radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility.Mice placed into aerated polystyrene boxes immediately before exposure to the entrance region of proton at the BNL Alternating Gradient Synchrotron (AGS) (1055 MeV/nucleon at the target, with a track-averaged LET =148.2 keV/μm at target). Doses of 0 and 3.0 Gy were delivered to a maximum of four mice at ~0.83 Gy/min in a single fraction. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Treatment ID:

TR000513

Treatment Summary:

A total of 27 seven-week-old C57BL/6 female mice randomly into 6 groups, and then whole body being exposed to either gamma or proton radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility.Mice placed into aerated polystyrene boxes immediately before exposure to the entrance region of proton at the BNL Alternating Gradient Synchrotron (AGS) (1055 MeV/nucleon at the target, with a track-averaged LET =148.2 keV/μm at target). Doses of 0 and 3.0 Gy were delivered to a maximum of four mice at ~0.83 Gy/min in a single fraction. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Sample Preparation:

Sampleprep ID:	SP000506
Sampleprep Summary:	Step 1: After randomization of the samples, every preweighted intact frozen liver tissue was homogenized by a Tissue Tearsor (Model 985-370, BioSpec Product, Inc.) in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Sampleprep ID:

SP000506

Sampleprep Summary:

Step 1: After randomization of the samples, every preweighted intact frozen liver tissue was homogenized by a Tissue Tearsor (Model 985-370, BioSpec Product, Inc.) in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Analysis:

Analysis ID:	AN000744
Analysis Type:	NMR
Num Factors:	6
Num Metabolites:	72
Units:	µM/mg

NMR:

NMR ID:	NM000083
Analysis ID:	AN000744
Instrument Name:	Varian INOVA 800
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	800 MHz