Summary of Study ST000487

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000369. The data can be accessed directly via it's Project DOI: 10.21228/M8BW2Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000487
Study TitleImpacts of high-fat diet (HFD), high-carbohydrate diet (HCD) and high-fat-high-carbohydrate diet (HFHCD) on metabolites in a farmed cyprinid fish Megalobrama amblycephala.
Study TypeDietary imbalance experiment
Study Summary1H NMR-based metabolomic approach to measure the concentrations of metabolites in plasma and liver of four different diet groups: HFD, HCD, HFHCD and control. Multivariate statistical analyses were used to determine significantly changed metabolites between all group-pairs.
Institute
Huazhong Agricultural University
DepartmentFisheries
LaboratoryKey Lab of Freshwater Animal Breeding
Last NamePrathomya
First NamePanita
AddressHuazhong Agricultural University, Shizi Shan Jie, Hongshan District.
Emailp_prathomya@yahoo.com
Phone+8613477033229
Submit Date2016-10-08
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2017-10-03
Release Version1
Panita Prathomya Panita Prathomya
https://dx.doi.org/10.21228/M8BW2Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000369
Project DOI:doi: 10.21228/M8BW2Q
Project Title:Plasma and liver metabolomics studies
Project Type:NMR multivariate analysis
Project Summary:1H NMR-based metabolomics approach reveals metabolic alterations in response to dietary imbalances in Megalobrama amblycephala
Institute:Huazhong Agricultural University
Department:Fisheries
Laboratory:Key Lab of Freshwater Animal Breeding
Last Name:Prathomya
First Name:Panita
Address:Huazhong Agricultural University, Shizi Shan Jie, Hongshan District., Wuhan, Hubei, 430070, China
Email:p_prathomya@yahoo.com
Phone:13477033229
Funding Source:Natural Science Foundation of China (No. 31401976), the earmarked fund for the Fundament Research Funds for the Central Universities (2662015PY019) and Modern Agro-industry Technology Research System entitled ‘‘Staple Freshwater Fishery Industry Technology System

Subject:

Subject ID:SU000508
Subject Type:Animal
Subject Species:Megalobrama amblycephala
Taxonomy ID:75352
Species Group:Fish

Factors:

Subject type: Animal; Subject species: Megalobrama amblycephala (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA025352A33Control
SA025353A34Control
SA025354A12Control
SA025355A32Control
SA025356A35Control
SA025357A23Control
SA025358A14Control
SA025359A13Control
SA025360A22Control
SA025361A15Control
SA025362C21High carbohydrate
SA025363C13High carbohydrate
SA025364C12High carbohydrate
SA025365C22High carbohydrate
SA025366C34High carbohydrate
SA025367C35High carbohydrate
SA025368C33High carbohydrate
SA025369C32High carbohydrate
SA025370C31High carbohydrate
SA025371C11High carbohydrate
SA025372B14High fat diet
SA025373B13High fat diet
SA025374B35High fat diet
SA025375B11High fat diet
SA025376B21High fat diet
SA025377B15High fat diet
SA025378B34High fat diet
SA025379B32High fat diet
SA025380B33High fat diet
SA025381B31High fat diet
SA025382D24Highfat Highcarbohydrate
SA025383D31Highfat Highcarbohydrate
SA025384D32Highfat Highcarbohydrate
SA025385D23Highfat Highcarbohydrate
SA025386D11Highfat Highcarbohydrate
SA025387D13Highfat Highcarbohydrate
SA025388D14Highfat Highcarbohydrate
SA025389D15Highfat Highcarbohydrate
SA025390D21Highfat Highcarbohydrate
SA025391D22Highfat Highcarbohydrate
Showing results 1 to 40 of 40

Collection:

Collection ID:CO000502
Collection Summary:Blood samples were obtained following the standard protocol, using sodium heparin as anticoagulant. Liver tissue samples were weighed and put in tube then immediately flash-frozen with liquid nitrogen and stored at −80 °C till used for metabolomics analysis.
Collection Protocol Filename:Sample collection and NMR protocol.pdf
Sample Type:Plasma, Liver

Treatment:

Treatment ID:TR000522
Treatment Summary:Three tanks were randomly assigned to each diet group (3 replicates x 4 diet-groups): control (C; containing 8.0% fat and 26.7% carbohydrates), high fat diet (HFD; containing 12.2% fat and 27.8% carbohydrates), high carbohydrate diet (HCD; containing 8.3% fat and 34.1% carbohydrates) and high fat high carbohydrate diet (HFHCD; containing 10.4% fat and 30.9% carbohydrates.

Sample Preparation:

Sampleprep ID:SP000515
Sampleprep Summary:Each plasma sample (170 μL) was mixed with 340 μL phosphate buffer (45 mM, pH 7.47, 50% D2O) containing 0.9% NaCl in a 5 mm NMR tube and used directly for the NMR analysis. Liver tissues (about 50 mg) were homogenized in cold methanol and water (v/v=2:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). The supernatant (550uL) was collected after centrifugation (16 099 g, 4°C, 10 min). The extraction was repeated thrice using the same procedure. The supernatants collected from the above procedure were mixed together and freeze-dried after the removal of methanol in vacuo. Then the residue was dissolved in 600 μL of phosphate buffer (0.15 M, pH 7.38, 80% D2O) containing 0.001% TSP and 0.01% NaN3. The supernatant (550 μL) was transferred into a 5 mm NMR tube.

Analysis:

Analysis ID:AN000753
Analysis Type:NMR
Num Factors:4

NMR:

NMR ID:NM000087
Analysis ID:AN000753
Instrument Name:Bruker AVance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Field Frequency Lock:Deuterium
Standard Concentration:0.058mM
Spectrometer Frequency:600 MHz
NMR Probe:cryo, inverse
NMR Solvent:D2O
NMR Tube Size:5mm x 7 in
Shimming Method:topshim
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