Summary of Study ST000488

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000370. The data can be accessed directly via it's Project DOI: 10.21228/M87604 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000488
Study Title	Sleep apnea and cardiovascular metabiltes carnitine, tma, tmao, betain, choline in plasma
Study Summary	Sleep apnea and cardiovascular metabiltes carnitine, tma, tmao, betain, choline in plasma
Institute	Mayo Clinic
Laboratory	Mayo Clinic Metabolomics Resource Core
Last Name	Pak
First Name	Victoria
Address	unknown
Email	victoria.pak@yale.edu
Phone	203-737-5065
Submit Date	2016-10-14
Analysis Type Detail	LC-MS
Release Date	2018-10-10
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000370
Project DOI:	doi: 10.21228/M87604
Project Title:	Mayo Metabolomics Pilot and Feasibility Award: Mechanisms of sleep apnea and cardiovascular disease
Project Type:	MS targeted analysis
Project Summary:	Targeted Metabolic and Biological Markers of Sleepiness Symptoms in Sleep apnea and cardiovascular disease
Institute:	Mayo Clinic
Laboratory:	Mayo Clinic Metabolomics Resource Core
Last Name:	Pak
First Name:	Victoria
Address:	unknown
Email:	victoria.pak@yale.edu
Phone:	203-737-5065

Subject:

Subject ID:	SU000509
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Disease
SA025392	ms5666-12	cardiovascular disease
SA025393	ms5666-11	cardiovascular disease
SA025394	ms5666-14	cardiovascular disease
SA025395	ms5666-16	cardiovascular disease
SA025396	ms5666-1	cardiovascular disease
SA025397	ms5666-10	cardiovascular disease
SA025398	ms5666-15	cardiovascular disease
SA025399	ms5666-13	cardiovascular disease
SA025400	ms5666-3	cardiovascular disease
SA025401	ms5666-9	cardiovascular disease
SA025402	ms5666-2	cardiovascular disease
SA025403	ms5666-4	cardiovascular disease
SA025404	ms5666-5	cardiovascular disease
SA025405	ms5666-8	cardiovascular disease
SA025406	ms5666-7	cardiovascular disease
SA025407	ms5666-6	cardiovascular disease
SA025408	ms5666-31	sleep apnea
SA025409	ms5666-30	sleep apnea
SA025410	ms5666-29	sleep apnea
SA025411	ms5666-32	sleep apnea
SA025412	ms5666-36	sleep apnea
SA025413	ms5666-28	sleep apnea
SA025414	ms5666-35	sleep apnea
SA025415	ms5666-34	sleep apnea
SA025416	ms5666-33	sleep apnea
SA025417	ms5666-19	sleep apnea
SA025418	ms5666-21	sleep apnea
SA025419	ms5666-20	sleep apnea
SA025420	ms5666-18	sleep apnea
SA025421	ms5666-17	sleep apnea
SA025422	ms5666-22	sleep apnea
SA025423	ms5666-23	sleep apnea
SA025424	ms5666-26	sleep apnea
SA025425	ms5666-25	sleep apnea
SA025426	ms5666-24	sleep apnea
SA025427	ms5666-27	sleep apnea

Showing results 1 to 36 of 36

Showing results 1 to 36 of 36

Collection:

Collection ID:	CO000503
Collection Summary:	For the metabolic markers, 9 ml blood will be drawn into 3 EDTA tubes (3 ml each) and gently centrifuged at 4°C for 10 min; plasma aliquots were immediately frozen and stored at -80 °C; will be stored at -80ºC in the CTRC until all samples are collected and then sent to Mayo Clinic Metabolomics Core via LC-MS/MS for simultaneous detection and quantification of metabolites for analyses.
Sample Type:	Blood

Treatment:

Treatment ID:	TR000523
Treatment Summary:	Recruitment of subjects will take place from the clinic population in collaboration with the Penn Sleep Center. Patients newly diagnosed with OSA will be solicited for via an overnight sleep study, which is general monitoring of sleep and variety of body functions during sleep. This includes brain (EEG), eye (EOG), and muscle activity and heart rhythm. The diagnosis of OSA will be established by full sleep study. First, the PI will screen for eligibility and obtain informed consent. We will assess height, weight, medical history, demographics. EDS will be measured subjectively on the night of enrollment in the study and determined using the Epworth Sleepiness Scale. The information regarding gender, BMI, smoking, age, medications (name/duration/frequency), and presence of comorbidities (e.g. hypertension, CVD defined by doctor diagnosis of myocardial infarction, stroke, or heart failure) will then be obtained via the electronic medical record. We will also ask subjects whether they exercise by asking participants the yes/no question: “During the past month, did you participate in any physical activities or exercises?” Second, the subject will undergo diagnostic PSG and we will enforce fasting prior to bedtime. Third, biological samples will be collected in the morning after the sleep study. Patients who have agreed to participate will undergo a blood draw (20 ml) and urine collection (30 ml) on the morning after PSG. Laboratory analysis of the biological samples will be conducted in collaboration with the Clinical Translation Research Center (CTRC). Finally, subjects will have 1 or 2 practice sessions with the PVT prior to administration of the task. PVT test will be conducted 3 times, at approximately 10:00, 12:00, and 14:00 and automatically scored. This will be done on the day after the sleep study.

Treatment ID:

TR000523

Treatment Summary:

Recruitment of subjects will take place from the clinic population in collaboration with the Penn Sleep Center. Patients newly diagnosed with OSA will be solicited for via an overnight sleep study, which is general monitoring of sleep and variety of body functions during sleep. This includes brain (EEG), eye (EOG), and muscle activity and heart rhythm. The diagnosis of OSA will be established by full sleep study. First, the PI will screen for eligibility and obtain informed consent. We will assess height, weight, medical history, demographics. EDS will be measured subjectively on the night of enrollment in the study and determined using the Epworth Sleepiness Scale. The information regarding gender, BMI, smoking, age, medications (name/duration/frequency), and presence of comorbidities (e.g. hypertension, CVD defined by doctor diagnosis of myocardial infarction, stroke, or heart failure) will then be obtained via the electronic medical record. We will also ask subjects whether they exercise by asking participants the yes/no question: “During the past month, did you participate in any physical activities or exercises?” Second, the subject will undergo diagnostic PSG and we will enforce fasting prior to bedtime. Third, biological samples will be collected in the morning after the sleep study. Patients who have agreed to participate will undergo a blood draw (20 ml) and urine collection (30 ml) on the morning after PSG. Laboratory analysis of the biological samples will be conducted in collaboration with the Clinical Translation Research Center (CTRC). Finally, subjects will have 1 or 2 practice sessions with the PVT prior to administration of the task. PVT test will be conducted 3 times, at approximately 10:00, 12:00, and 14:00 and automatically scored. This will be done on the day after the sleep study.

Sample Preparation:

Sampleprep ID:	SP000516
Sampleprep Summary:	TMAO panel

Combined analysis:

Analysis ID	AN000754
Analysis type	MS
Chromatography type	HILIC
Chromatography system	Cohesive TX2
Column	Altma HP HILIC (150 x 2.1mm,5um)
MS Type	ESI
MS instrument type	Triple quadrupole
MS instrument name	ABI Sciex 6500 QTrap
Ion Mode	POSITIVE
Units	micromolar

Chromatography:

Chromatography ID:	CH000540
Instrument Name:	Cohesive TX2
Column Name:	Altma HP HILIC (150 x 2.1mm,5um)
Chromatography Type:	HILIC

MS:

MS ID:	MS000666
Analysis ID:	AN000754
Instrument Name:	ABI Sciex 6500 QTrap
Instrument Type:	Triple quadrupole
MS Type:	ESI
Ion Mode:	POSITIVE