Summary of Study ST000491
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000370. The data can be accessed directly via it's Project DOI: 10.21228/M87604 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000491 |
| Study Title | Sleep apnea and cardiovascular samples amino acid metabolites |
| Study Summary | Sleep apnea and cardiovascular samples amino acid metabolites |
| Institute | Mayo Clinic |
| Laboratory | Mayo Clinic Metabolomics Resource Core |
| Last Name | Pak |
| First Name | Victoria |
| Address | unknown |
| victoria.pak@yale.edu | |
| Phone | 203-737-5065 |
| Submit Date | 2016-10-14 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000370 |
| Project DOI: | doi: 10.21228/M87604 |
| Project Title: | Mayo Metabolomics Pilot and Feasibility Award: Mechanisms of sleep apnea and cardiovascular disease |
| Project Type: | MS targeted analysis |
| Project Summary: | Targeted Metabolic and Biological Markers of Sleepiness Symptoms in Sleep apnea and cardiovascular disease |
| Institute: | Mayo Clinic |
| Laboratory: | Mayo Clinic Metabolomics Resource Core |
| Last Name: | Pak |
| First Name: | Victoria |
| Address: | unknown |
| Email: | victoria.pak@yale.edu |
| Phone: | 203-737-5065 |
Subject:
| Subject ID: | SU000512 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Disease |
|---|---|---|
| SA025500 | ms5686-12 | cardiovascular disease |
| SA025501 | ms5686-11 | cardiovascular disease |
| SA025502 | ms5686-14 | cardiovascular disease |
| SA025503 | ms5686-16 | cardiovascular disease |
| SA025504 | ms5686-1 | cardiovascular disease |
| SA025505 | ms5686-10 | cardiovascular disease |
| SA025506 | ms5686-15 | cardiovascular disease |
| SA025507 | ms5686-13 | cardiovascular disease |
| SA025508 | ms5686-3 | cardiovascular disease |
| SA025509 | ms5686-9 | cardiovascular disease |
| SA025510 | ms5686-2 | cardiovascular disease |
| SA025511 | ms5686-4 | cardiovascular disease |
| SA025512 | ms5686-5 | cardiovascular disease |
| SA025513 | ms5686-8 | cardiovascular disease |
| SA025514 | ms5686-7 | cardiovascular disease |
| SA025515 | ms5686-6 | cardiovascular disease |
| SA025516 | ms5686-31 | sleep apnea |
| SA025517 | ms5686-30 | sleep apnea |
| SA025518 | ms5686-29 | sleep apnea |
| SA025519 | ms5686-32 | sleep apnea |
| SA025520 | ms5686-36 | sleep apnea |
| SA025521 | ms5686-28 | sleep apnea |
| SA025522 | ms5686-35 | sleep apnea |
| SA025523 | ms5686-34 | sleep apnea |
| SA025524 | ms5686-33 | sleep apnea |
| SA025525 | ms5686-19 | sleep apnea |
| SA025526 | ms5686-21 | sleep apnea |
| SA025527 | ms5686-20 | sleep apnea |
| SA025528 | ms5686-18 | sleep apnea |
| SA025529 | ms5686-17 | sleep apnea |
| SA025530 | ms5686-22 | sleep apnea |
| SA025531 | ms5686-23 | sleep apnea |
| SA025532 | ms5686-26 | sleep apnea |
| SA025533 | ms5686-25 | sleep apnea |
| SA025534 | ms5686-24 | sleep apnea |
| SA025535 | ms5686-27 | sleep apnea |
| Showing results 1 to 36 of 36 |
Collection:
| Collection ID: | CO000506 |
| Collection Summary: | For the metabolic markers, 9 ml blood will be drawn into 3 EDTA tubes (3 ml each) and gently centrifuged at 4°C for 10 min; plasma aliquots were immediately frozen and stored at -80 °C; will be stored at -80ºC in the CTRC until all samples are collected and then sent to Mayo Clinic Metabolomics Core via LC-MS/MS for simultaneous detection and quantification of metabolites for analyses. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000526 |
| Treatment Summary: | Recruitment of subjects will take place from the clinic population in collaboration with the Penn Sleep Center. Patients newly diagnosed with OSA will be solicited for via an overnight sleep study, which is general monitoring of sleep and variety of body functions during sleep. This includes brain (EEG), eye (EOG), and muscle activity and heart rhythm. The diagnosis of OSA will be established by full sleep study. First, the PI will screen for eligibility and obtain informed consent. We will assess height, weight, medical history, demographics. EDS will be measured subjectively on the night of enrollment in the study and determined using the Epworth Sleepiness Scale. The information regarding gender, BMI, smoking, age, medications (name/duration/frequency), and presence of comorbidities (e.g. hypertension, CVD defined by doctor diagnosis of myocardial infarction, stroke, or heart failure) will then be obtained via the electronic medical record. We will also ask subjects whether they exercise by asking participants the yes/no question: “During the past month, did you participate in any physical activities or exercises?” Second, the subject will undergo diagnostic PSG and we will enforce fasting prior to bedtime. Third, biological samples will be collected in the morning after the sleep study. Patients who have agreed to participate will undergo a blood draw (20 ml) and urine collection (30 ml) on the morning after PSG. Laboratory analysis of the biological samples will be conducted in collaboration with the Clinical Translation Research Center (CTRC). Finally, subjects will have 1 or 2 practice sessions with the PVT prior to administration of the task. PVT test will be conducted 3 times, at approximately 10:00, 12:00, and 14:00 and automatically scored. This will be done on the day after the sleep study. |
Sample Preparation:
| Sampleprep ID: | SP000519 |
| Sampleprep Summary: | Amino Acid Panel |
Combined analysis:
| Analysis ID | AN000757 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | POSITIVE |
| Units | micromolar |
Chromatography:
| Chromatography ID: | CH000543 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000669 |
| Analysis ID: | AN000757 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
