Summary of Study ST000492

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000370. The data can be accessed directly via it's Project DOI: 10.21228/M87604 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000492
Study TitleSleep apnea and cardiovascular samples ceramide concentrations
Study SummarySleep apnea and cardiovascular samples ceramide concentrations
Institute
Mayo Clinic
LaboratoryMayo Clinic Metabolomics Resource Core
Last NamePak
First NameVictoria
Addressunknown
Emailvictoria.pak@yale.edu
Phone203-737-5065
Submit Date2016-10-14
Analysis Type DetailLC-MS
Release Date2018-10-10
Release Version1
Victoria Pak Victoria Pak
https://dx.doi.org/10.21228/M87604
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000370
Project DOI:doi: 10.21228/M87604
Project Title:Mayo Metabolomics Pilot and Feasibility Award: Mechanisms of sleep apnea and cardiovascular disease
Project Type:MS targeted analysis
Project Summary:Targeted Metabolic and Biological Markers of Sleepiness Symptoms in Sleep apnea and cardiovascular disease
Institute:Mayo Clinic
Laboratory:Mayo Clinic Metabolomics Resource Core
Last Name:Pak
First Name:Victoria
Address:unknown
Email:victoria.pak@yale.edu
Phone:203-737-5065

Subject:

Subject ID:SU000513
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Disease
SA025536ms5687-12cardiovascular disease
SA025537ms5687-11cardiovascular disease
SA025538ms5687-14cardiovascular disease
SA025539ms5687-16cardiovascular disease
SA025540ms5687-1cardiovascular disease
SA025541ms5687-10cardiovascular disease
SA025542ms5687-15cardiovascular disease
SA025543ms5687-13cardiovascular disease
SA025544ms5687-3cardiovascular disease
SA025545ms5687-9cardiovascular disease
SA025546ms5687-2cardiovascular disease
SA025547ms5687-4cardiovascular disease
SA025548ms5687-5cardiovascular disease
SA025549ms5687-8cardiovascular disease
SA025550ms5687-7cardiovascular disease
SA025551ms5687-6cardiovascular disease
SA025552ms5687-31sleep apnea
SA025553ms5687-30sleep apnea
SA025554ms5687-29sleep apnea
SA025555ms5687-32sleep apnea
SA025556ms5687-36sleep apnea
SA025557ms5687-28sleep apnea
SA025558ms5687-35sleep apnea
SA025559ms5687-34sleep apnea
SA025560ms5687-33sleep apnea
SA025561ms5687-19sleep apnea
SA025562ms5687-21sleep apnea
SA025563ms5687-20sleep apnea
SA025564ms5687-18sleep apnea
SA025565ms5687-17sleep apnea
SA025566ms5687-22sleep apnea
SA025567ms5687-23sleep apnea
SA025568ms5687-26sleep apnea
SA025569ms5687-25sleep apnea
SA025570ms5687-24sleep apnea
SA025571ms5687-27sleep apnea
Showing results 1 to 36 of 36

Collection:

Collection ID:CO000507
Collection Summary:For the metabolic markers, 9 ml blood will be drawn into 3 EDTA tubes (3 ml each) and gently centrifuged at 4°C for 10 min; plasma aliquots were immediately frozen and stored at -80 °C; will be stored at -80ºC in the CTRC until all samples are collected and then sent to Mayo Clinic Metabolomics Core via LC-MS/MS for simultaneous detection and quantification of metabolites for analyses.
Sample Type:Blood

Treatment:

Treatment ID:TR000527
Treatment Summary:Recruitment of subjects will take place from the clinic population in collaboration with the Penn Sleep Center. Patients newly diagnosed with OSA will be solicited for via an overnight sleep study, which is general monitoring of sleep and variety of body functions during sleep. This includes brain (EEG), eye (EOG), and muscle activity and heart rhythm. The diagnosis of OSA will be established by full sleep study. First, the PI will screen for eligibility and obtain informed consent. We will assess height, weight, medical history, demographics. EDS will be measured subjectively on the night of enrollment in the study and determined using the Epworth Sleepiness Scale. The information regarding gender, BMI, smoking, age, medications (name/duration/frequency), and presence of comorbidities (e.g. hypertension, CVD defined by doctor diagnosis of myocardial infarction, stroke, or heart failure) will then be obtained via the electronic medical record. We will also ask subjects whether they exercise by asking participants the yes/no question: “During the past month, did you participate in any physical activities or exercises?” Second, the subject will undergo diagnostic PSG and we will enforce fasting prior to bedtime. Third, biological samples will be collected in the morning after the sleep study. Patients who have agreed to participate will undergo a blood draw (20 ml) and urine collection (30 ml) on the morning after PSG. Laboratory analysis of the biological samples will be conducted in collaboration with the Clinical Translation Research Center (CTRC). Finally, subjects will have 1 or 2 practice sessions with the PVT prior to administration of the task. PVT test will be conducted 3 times, at approximately 10:00, 12:00, and 14:00 and automatically scored. This will be done on the day after the sleep study.

Sample Preparation:

Sampleprep ID:SP000520
Sampleprep Summary:ceramide concentrations

Combined analysis:

Analysis ID AN000758
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity
Column AMT HALO HILIC 2 (150 x 4.6mm,2.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva QQQ
Ion Mode POSITIVE
Units micromolar

Chromatography:

Chromatography ID:CH000544
Instrument Name:Waters Acquity
Column Name:AMT HALO HILIC 2 (150 x 4.6mm,2.7um)
Chromatography Type:HILIC

MS:

MS ID:MS000670
Analysis ID:AN000758
Instrument Name:Thermo Quantiva QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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