Summary of Study ST000498

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000375. The data can be accessed directly via it's Project DOI: 10.21228/M8KC7M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000498
Study TitleCocaine administration and withdrawal (part I)
Study Typetreated and control
Study SummaryThe goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to cocaine administration and withdrawal as compared to control
Institute
University of Florida
DepartmentSECIM
Last NameDownes
First NameDaniel
Address1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611
Emaildaniel.downes@neurosurgery.ufl.edu
Phone(321)303-6523
Submit Date2016-10-11
Num Groups5
Total Subjects12
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-11-20
Release Version1
Daniel Downes Daniel Downes
https://dx.doi.org/10.21228/M8KC7M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000375
Project DOI:doi: 10.21228/M8KC7M
Project Title:Drug's effect on cerebral metabolic pool sizes
Project Summary:The goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to drug administration and withdrawal
Institute:University of Florida
Department:Biochemistry and Molecular Biology
Last Name:Joanna
First Name:Long
Address:1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611
Email:jrlong@mbi.ufl.edu
Phone:none
Funding Source:UF

Subject:

Subject ID:SU000519
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Subject Comments:Long Evans Rat
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id drug admin
SA025797LongDNP_001acute
SA025798LongDNP_003acute
SA025799LongDNP_002acute
SA025800LongDNP_006acute control
SA025801LongDNP_004acute control
SA025802LongDNP_005acute control
SA025803LongDNP_009withdrawal
SA025804LongDNP_007withdrawal
SA025805LongDNP_008withdrawal
SA025806LongDNP_012withdrawal control
SA025807LongDNP_011withdrawal control
SA025808LongDNP_010withdrawal control
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000513
Collection Summary:harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2
Sample Type:Tissue

Treatment:

Treatment ID:TR000533
Treatment Summary:Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal

Sample Preparation:

Sampleprep ID:SP000526
Sampleprep Summary:none
Sampleprep Protocol Filename:SB_OA_Assay.pdf

Combined analysis:

Analysis ID AN000766
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva QQQ
Ion Mode POSITIVE
Units nmol/mg dry powder

Chromatography:

Chromatography ID:CH000551
Methods Filename:SB_OA_Assay.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:45
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS000678
Analysis ID:AN000766
Instrument Name:Thermo Quantiva QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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