Summary of Study ST000498
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000375. The data can be accessed directly via it's Project DOI: 10.21228/M8KC7M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000498 |
| Study Title | Cocaine administration and withdrawal (part I) |
| Study Type | treated and control |
| Study Summary | The goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to cocaine administration and withdrawal as compared to control |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Downes |
| First Name | Daniel |
| Address | 1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611 |
| daniel.downes@neurosurgery.ufl.edu | |
| Phone | (321)303-6523 |
| Submit Date | 2016-10-11 |
| Num Groups | 5 |
| Total Subjects | 12 |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-11-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000375 |
| Project DOI: | doi: 10.21228/M8KC7M |
| Project Title: | Drug's effect on cerebral metabolic pool sizes |
| Project Summary: | The goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to drug administration and withdrawal |
| Institute: | University of Florida |
| Department: | Biochemistry and Molecular Biology |
| Last Name: | Joanna |
| First Name: | Long |
| Address: | 1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611 |
| Email: | jrlong@mbi.ufl.edu |
| Phone: | none |
| Funding Source: | UF |
Subject:
| Subject ID: | SU000519 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Subject Comments: | Long Evans Rat |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | drug admin |
|---|---|---|
| SA025797 | LongDNP_001 | acute |
| SA025798 | LongDNP_003 | acute |
| SA025799 | LongDNP_002 | acute |
| SA025800 | LongDNP_006 | acute control |
| SA025801 | LongDNP_004 | acute control |
| SA025802 | LongDNP_005 | acute control |
| SA025803 | LongDNP_009 | withdrawal |
| SA025804 | LongDNP_007 | withdrawal |
| SA025805 | LongDNP_008 | withdrawal |
| SA025806 | LongDNP_012 | withdrawal control |
| SA025807 | LongDNP_011 | withdrawal control |
| SA025808 | LongDNP_010 | withdrawal control |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000513 |
| Collection Summary: | harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2 |
| Sample Type: | Tissue |
Treatment:
| Treatment ID: | TR000533 |
| Treatment Summary: | Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal |
Sample Preparation:
| Sampleprep ID: | SP000526 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | SB_OA_Assay.pdf |
Chromatography:
| Chromatography ID: | CH000551 |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN000766 |
| Laboratory Name: | Sanford Burnham Prebys |
| Analysis Type: | MS |
| Instrument Name: | Thermo Quantiva |
| Chromatography ID: | CH000551 |
| Num Factors: | 4 |
| Num Metabolites: | 7 |
| Rt Units: | Minutes |
| Units: | nmol/mg dry powder |
