Summary of Study ST000499
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000376. The data can be accessed directly via it's Project DOI: 10.21228/M8FP4B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000499 |
| Study Title | NAD+ in neurodegeneration |
| Study Type | Baseline comparison of NAD metabolism in iPSC-derived neurons |
| Study Summary | Differentiated iPSCs from patients and controls and knockout cells |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Deleidi |
| First Name | Michela |
| Address | Otfried-Müller. Str 23, 72076, Tübingen, Germany |
| michela.deleidi@dzne.de | |
| Phone | none |
| Submit Date | 2016-10-11 |
| Num Groups | 3 |
| Total Subjects | 17 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-11-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000376 |
| Project DOI: | doi: 10.21228/M8FP4B |
| Project Title: | The role of GBA1 in Parkinson's disease |
| Project Summary: | The role of GBA1 in Parkinson's disease |
| Institute: | German Center for Neurodegenerative Diseases |
| Department: | Mitochondria and Inflammation in Neurodegeneration |
| Last Name: | Deleidi |
| First Name: | Michela |
| Address: | Otfried-Müller. Str 23, 72076, Tübingen, Germany |
| Email: | michela.deleidi@dzne.de |
| Phone: | none |
| Funding Source: | DFG |
Subject:
| Subject ID: | SU000520 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA025809 | iPSC_08 | KO/KO |
| SA025810 | iPSC_09 | KO/KO |
| SA025811 | iPSC_03 | KO/KO |
| SA025812 | iPSC_16 | WT/L444P |
| SA025813 | iPSC_14 | WT/L444P |
| SA025814 | iPSC_04 | WT/N370S |
| SA025815 | iPSC_12 | WT/N370S |
| SA025816 | iPSC_10 | WT/N370S |
| SA025817 | iPSC_01 | WT/N370S |
| SA025818 | iPSC_06 | WT/N370S |
| SA025819 | iPSC_02 | WT/WT |
| SA025820 | iPSC_17 | WT/WT |
| SA025821 | iPSC_15 | WT/WT |
| SA025822 | iPSC_11 | WT/WT |
| SA025823 | iPSC_05 | WT/WT |
| SA025824 | iPSC_07 | WT/WT |
| SA025825 | iPSC_13 | WT/WT |
| Showing results 1 to 17 of 17 |
Collection:
| Collection ID: | CO000514 |
| Collection Summary: | Cells were washed once with cold PBS, scraped off the plate in 1 ml cold PBS, transfered in pre-cooled 50 ml falcons and pelleted by centrifugation 3000rpm at 4°C. PBS was taken off and pellets were then snap frozen in liquid N2 and stored at -80°C. |
| Sample Type: | Neurons |
Treatment:
| Treatment ID: | TR000534 |
| Treatment Summary: | 80% confluence, 48 hrs after last media change, differentiation medium (Neurobasal/B27/L-glut containing medium supplemented with BDNF, ascorbic acid , GDNF, TGFβ3, dibutyryl cAMP and DAPT. |
Sample Preparation:
| Sampleprep ID: | SP000527 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Combined analysis:
| Analysis ID | AN000767 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Thermo Hypercarb (50 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | nmol/mg protein |
Chromatography:
| Chromatography ID: | CH000552 |
| Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000679 |
| Analysis ID: | AN000767 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
