Summary of Study ST000499

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000376. The data can be accessed directly via it's Project DOI: 10.21228/M8FP4B This work is supported by NIH grant, U2C- DK119886.

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Study IDST000499
Study TitleNAD+ in neurodegeneration
Study TypeBaseline comparison of NAD metabolism in iPSC-derived neurons
Study SummaryDifferentiated iPSCs from patients and controls and knockout cells
Institute
University of Florida
DepartmentSECIM
Last NameDeleidi
First NameMichela
AddressOtfried-Müller. Str 23, 72076, Tübingen, Germany
Emailmichela.deleidi@dzne.de
Phonenone
Submit Date2016-10-11
Num Groups3
Total Subjects17
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-11-20
Release Version1
Michela Deleidi Michela Deleidi
https://dx.doi.org/10.21228/M8FP4B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000376
Project DOI:doi: 10.21228/M8FP4B
Project Title:The role of GBA1 in Parkinson's disease
Project Summary:The role of GBA1 in Parkinson's disease
Institute:German Center for Neurodegenerative Diseases
Department:Mitochondria and Inflammation in Neurodegeneration
Last Name:Deleidi
First Name:Michela
Address:Otfried-Müller. Str 23, 72076, Tübingen, Germany
Email:michela.deleidi@dzne.de
Phone:none
Funding Source:DFG

Subject:

Subject ID:SU000520
Subject Type:Cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA025809iPSC_08KO/KO
SA025810iPSC_09KO/KO
SA025811iPSC_03KO/KO
SA025812iPSC_16WT/L444P
SA025813iPSC_14WT/L444P
SA025814iPSC_04WT/N370S
SA025815iPSC_12WT/N370S
SA025816iPSC_10WT/N370S
SA025817iPSC_01WT/N370S
SA025818iPSC_06WT/N370S
SA025819iPSC_02WT/WT
SA025820iPSC_17WT/WT
SA025821iPSC_15WT/WT
SA025822iPSC_11WT/WT
SA025823iPSC_05WT/WT
SA025824iPSC_07WT/WT
SA025825iPSC_13WT/WT
Showing results 1 to 17 of 17

Collection:

Collection ID:CO000514
Collection Summary:Cells were washed once with cold PBS, scraped off the plate in 1 ml cold PBS, transfered in pre-cooled 50 ml falcons and pelleted by centrifugation 3000rpm at 4°C. PBS was taken off and pellets were then snap frozen in liquid N2 and stored at -80°C.
Sample Type:Neurons

Treatment:

Treatment ID:TR000534
Treatment Summary:80% confluence, 48 hrs after last media change, differentiation medium (Neurobasal/B27/L-glut containing medium supplemented with BDNF, ascorbic acid , GDNF, TGFβ3, dibutyryl cAMP and DAPT.

Sample Preparation:

Sampleprep ID:SP000527
Sampleprep Summary:none
Sampleprep Protocol Filename:SB_NAD-ATP_Assay_Agilent.pdf

Combined analysis:

Analysis ID AN000767
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Thermo Hypercarb (50 x 2.1mm, 3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units nmol/mg protein

Chromatography:

Chromatography ID:CH000552
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000679
Analysis ID:AN000767
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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