Summary of Study ST000505

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000505
Study TitleTimecourse of Amino Acid Metabolites in LNCaP Cell pellets
Study Typetimecourse
Study SummaryTimecourse of Amino Acid Metabolites in LNCaP Cell pellets
Institute
Mayo Clinic
LaboratoryMayo Metabolomics Core
Last NameFrigo
First NameDaniel
AddressDepartment of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001
Emailfrigo@uh.edu
Phone832-842-8824
Submit Date2016-10-21
Analysis Type DetailLC-MS
Release Date2018-10-10
Release Version1
Daniel Frigo Daniel Frigo
https://dx.doi.org/10.21228/M83C78
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000371
Project DOI:doi: 10.21228/M83C78
Project Title:Metabolic Dissection of Aberrant Glutamine Metabolism in Prostate Cancer
Project Type:MS targeted analysis
Project Summary:In this project, we propose to define the specific role(s) of three crucial oncogenic signaling cascades (androgen receptor, Myc and AMPK) in regulating glutamine metabolism in prostate cancer using 13C-labeled glutamine with mass spectrometry-based metabolic flux analyses.
Institute:Mayo Clinic
Laboratory:Mayo Clinic Metabolomics Resource Core
Last Name:Frigo
First Name:Daniel
Address:Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001
Email:frigo@uh.edu
Phone:832-842-8824

Subject:

Subject ID:SU000527
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Factor 13C media incubation time
SA026266ms5459-6treated-100 pM R1881 24 hours
SA026267ms5459-2treated-100 pM R1881 24 hours
SA026268ms5459-13treated-100 pM R1881 72 hours
SA026269ms5459-9treated-100 pM R1881 72 hours
SA026262ms5459-7treated-10 nM R1881 24 hours
SA026263ms5459-4treated-10 nM R1881 24 hours
SA026264ms5459-11treated-10 nM R1881 72 hours
SA026265ms5459-14treated-10 nM R1881 72 hours
SA026260ms5459-3treated-1 nM R1881 24 hours
SA026261ms5459-10treated-1 nM R1881 72 hours
SA026270ms5459-1treated-vehicle (ethanol) 24 hours
SA026271ms5459-5treated-vehicle (ethanol) 24 hours
SA026272ms5459-8treated-vehicle (ethanol) 72 hours
SA026273ms5459-12treated-vehicle (ethanol) 72 hours
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000521
Collection Summary:LNCaP cell media
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000541
Treatment Summary:LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours.

Sample Preparation:

Sampleprep ID:SP000534
Sampleprep Summary:Concentration of Amino Acids metabolites in cell media

Combined analysis:

Analysis ID AN000774
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units micromolar

Chromatography:

Chromatography ID:CH000554
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000681
Analysis ID:AN000774
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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