Summary of Study ST000506
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000506 |
| Study Title | Timecourse of TCA panel in LNCaP Cells |
| Study Summary | Timecourse of TCA panel in LNCaP Cells |
| Institute | Mayo Clinic |
| Last Name | Frigo |
| First Name | Daniel |
| Address | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| frigo@uh.edu | |
| Phone | 832-842-8824 |
| Submit Date | 2016-10-21 |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000371 |
| Project DOI: | doi: 10.21228/M83C78 |
| Project Title: | Metabolic Dissection of Aberrant Glutamine Metabolism in Prostate Cancer |
| Project Type: | MS targeted analysis |
| Project Summary: | In this project, we propose to define the specific role(s) of three crucial oncogenic signaling cascades (androgen receptor, Myc and AMPK) in regulating glutamine metabolism in prostate cancer using 13C-labeled glutamine with mass spectrometry-based metabolic flux analyses. |
| Institute: | Mayo Clinic |
| Laboratory: | Mayo Clinic Metabolomics Resource Core |
| Last Name: | Frigo |
| First Name: | Daniel |
| Address: | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| Email: | frigo@uh.edu |
| Phone: | 832-842-8824 |
Subject:
| Subject ID: | SU000528 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA026280 | sample # 9 | 100 pM R1881 |
| SA026281 | sample # 6 | 100 pM R1881 |
| SA026282 | sample # 2 | 100 pM R1881 |
| SA026283 | sample # 13 | 100 pM R1881 |
| SA026276 | sample # 14 | 10 nM R1881 |
| SA026277 | sample # 7 | 10 nM R1881 |
| SA026278 | sample # 4 | 10 nM R1881 |
| SA026279 | sample # 11 | 10 nM R1881 |
| SA026274 | sample # 10 | 1 nM R1881 |
| SA026275 | sample # 3 | 1 nM R1881 |
| SA026284 | sample # 8 | vehicle (ethanol) |
| SA026285 | sample # 5 | vehicle (ethanol) |
| SA026286 | sample # 1 | vehicle (ethanol) |
| SA026287 | sample # 12 | vehicle (ethanol) |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000522 |
| Collection Summary: | LNCaP media |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000542 |
| Treatment Summary: | LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours. |
Sample Preparation:
| Sampleprep ID: | SP000535 |
| Sampleprep Summary: | TCA media concentrations |
Combined analysis:
| Analysis ID | AN000775 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | nmol/ml |
Chromatography:
| Chromatography ID: | CH000555 |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000682 |
| Analysis ID: | AN000775 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
