Summary of Study ST000541
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000396. The data can be accessed directly via it's Project DOI: 10.21228/M8VP5F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000541 |
| Study Title | Canine Diabetes - Preliminary Evaluation of Testing Methods |
| Study Type | Single time point blood collection |
| Study Summary | Blood was collected from diabetic dogs and healthy control dogs. Diabetic dogs and control dogs were breed matched where possible. Timing of blood collection after a meal was matched as best as possible. Serum was frozen at -80C. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | O'Kell |
| First Name | Allison |
| Address | 2015 SW 16th Ave |
| aokell@ufl.edu | |
| Phone | 352-392-2235 |
| Submit Date | 2017-01-10 |
| Num Groups | 2 |
| Total Subjects | 24 |
| Publications | O'Kell AL1, Garrett TJ2, et al. "Untargeted metabolomic analysis in naturally occurring canine diabetes mellitus identifies similarities to human Type 1 Diabetes." Sci Rep. 2017 Aug 25;7(1):9467. doi: 10.1038/s41598-017-09908-5. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-04-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000396 |
| Project DOI: | doi: 10.21228/M8VP5F |
| Project Title: | Utility of canine diabetes as a model for human type 1 diabetes |
| Project Summary: | We are examining the etiopathogenesis of canine diabetes to determine the relevance of these animals as a naturally occurring model of human type 1 diabetes. We will undertake phenotypic, metabolic, and immunologic investigations in diabetic dogs to better characterize their diabetes and attempt to identify potential biomarkers of a pre-diabetic state. |
| Institute: | University of Florida |
| Department: | Diabetes Institute (Wasserfall/Atkinson)/College of Veterinary Medicine (O'Kell) |
| Laboratory: | Atkinson Lab |
| Last Name: | O'Kell |
| First Name: | Allison |
| Address: | 2015 SW 16th Ave. |
| Email: | aokell@ufl.edu |
| Phone: | 352-392-2235 |
Subject:
| Subject ID: | SU000563 |
| Subject Type: | Animal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
| Age Or Age Range: | 1 year - 15 years |
| Weight Or Weight Range: | 5 kg - 40 kg |
| Gender: | Male Neutered or Female Spayed |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Canis lupus familiaris (Factor headings shown in green)
| mb_sample_id | local_sample_id | Health Status | Gender | Fasting Status | Breed | Size (S/M/L) |
|---|---|---|---|---|---|---|
| SA027995 | 213269 | Diabetic | Neutered male | Fasted | Labrador retriever | Large |
| SA027996 | 313390 | Diabetic | Neutered male | Fasted | Miniature schnauzer | Small |
| SA027997 | 325103 | Diabetic | Neutered male | Fasted | Mixed | Large |
| SA027998 | 216422 | Diabetic | Neutered male | Fed | Dachshund | Small |
| SA027999 | 309443 | Diabetic | Neutered male | Fed | Mixed | Medium |
| SA028000 | 321588 | Diabetic | Spayed female | Fasted | Dachshund | Small |
| SA028001 | 325942 | Diabetic | Spayed female | Fasted | Dachshund | Small |
| SA028002 | 213267 | Diabetic | Spayed female | Fasted | Labrador retriever | Large |
| SA028003 | 279436 | Diabetic | Spayed female | Fed | Labrador retriever | Large |
| SA028004 | 325643 | Diabetic | Spayed female | Fed | Labrador retriever | Large |
| SA028005 | 269031 | Diabetic | Spayed female | Fed | Mixed | Small |
| SA028006 | 323294 | Diabetic | Spayed female | Fed | Shih Tzu | Small |
| SA028007 | 290374 | Healthy | Neutered male | Fasted | Flat coated retriever | Large |
| SA028008 | 255293 | Healthy | Neutered male | Fasted | Labrador retriever | Large |
| SA028009 | 223169 | Healthy | Neutered male | Fed | Dachshund | Small |
| SA028010 | 290195 | Healthy | Neutered male | Fed | Labrador retriever | Large |
| SA028011 | 325932 | Healthy | Neutered male | Fed | Mixed | Medium |
| SA028012 | 296735 | Healthy | Neutered male | Fed | Mixed | Small |
| SA028013 | 326077 | Healthy | Neutered male | Fed | Shih Tzu | Small |
| SA028014 | 325866 | Healthy | Spayed female | Fasted | Dachshund | Small |
| SA028015 | 288261 | Healthy | Spayed female | Fasted | Dachshund | Small |
| SA028016 | 228415 | Healthy | Spayed female | Fasted | Labrador retriever | Large |
| SA028017 | 295029 | Healthy | Spayed female | Fasted | Miniature schnauzer | Small |
| SA028018 | 287971 | Healthy | Spayed female | Fed | Labrador retriever | Large |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO000557 |
| Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
| Sample Type: | Blood (serum) |
| Collection Method: | Peripheral venipuncture |
| Collection Location: | Jugular, saphenous, or cephalic vein |
| Collection Frequency: | Once |
| Storage Conditions: | Room temperature until centrifugation. Then -80 C immediately after separation from red cells. Serum was thawed once on ice, 100 uL aliquots taken, and aliquots re-frozen (at -80C) until use for metabolomics. |
| Collection Vials: | Red top with clot activator |
| Storage Vials: | Cryovials (polypropylene with silicone gasket - Fisher Brand) |
| Collection Tube Temp: | Room temperature |
| Blood Serum Or Plasma: | Serum |
Treatment:
| Treatment ID: | TR000577 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000570 |
| Sampleprep Summary: | See protocol |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000822 | AN000823 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific Q Exactive | Thermo Scientific Q Exactive |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH000587 |
| Chromatography Summary: | see protocol |
| Instrument Name: | Thermo Scientific Q Exactive |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000723 |
| Analysis ID: | AN000822 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000724 |
| Analysis ID: | AN000823 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
