Summary of Study ST000541

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000396. The data can be accessed directly via it's Project DOI: 10.21228/M8VP5F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST000541
Study Title	Canine Diabetes - Preliminary Evaluation of Testing Methods
Study Type	Single time point blood collection
Study Summary	Blood was collected from diabetic dogs and healthy control dogs. Diabetic dogs and control dogs were breed matched where possible. Timing of blood collection after a meal was matched as best as possible. Serum was frozen at -80C.
Institute	University of Florida
Department	SECIM
Last Name	O'Kell
First Name	Allison
Address	2015 SW 16th Ave
Email	aokell@ufl.edu
Phone	352-392-2235
Submit Date	2017-01-10
Num Groups	2
Total Subjects	24
Publications	O'Kell AL1, Garrett TJ2, et al. "Untargeted metabolomic analysis in naturally occurring canine diabetes mellitus identifies similarities to human Type 1 Diabetes." Sci Rep. 2017 Aug 25;7(1):9467. doi: 10.1038/s41598-017-09908-5.
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2018-04-10
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000396
Project DOI:	doi: 10.21228/M8VP5F
Project Title:	Utility of canine diabetes as a model for human type 1 diabetes
Project Summary:	We are examining the etiopathogenesis of canine diabetes to determine the relevance of these animals as a naturally occurring model of human type 1 diabetes. We will undertake phenotypic, metabolic, and immunologic investigations in diabetic dogs to better characterize their diabetes and attempt to identify potential biomarkers of a pre-diabetic state.
Institute:	University of Florida
Department:	Diabetes Institute (Wasserfall/Atkinson)/College of Veterinary Medicine (O'Kell)
Laboratory:	Atkinson Lab
Last Name:	O'Kell
First Name:	Allison
Address:	2015 SW 16th Ave.
Email:	aokell@ufl.edu
Phone:	352-392-2235

Subject:

Subject ID:	SU000563
Subject Type:	Animal
Subject Species:	Canis lupus familiaris
Taxonomy ID:	9615
Age Or Age Range:	1 year - 15 years
Weight Or Weight Range:	5 kg - 40 kg
Gender:	Male Neutered or Female Spayed
Species Group:	Mammals

Factors:

Subject type: Animal; Subject species: Canis lupus familiaris (Factor headings shown in green)

mb_sample_id	local_sample_id	Health Status	Gender	Fasting Status	Breed	Size (S/M/L)
SA027995	213269	Diabetic	Neutered male	Fasted	Labrador retriever	Large
SA027996	313390	Diabetic	Neutered male	Fasted	Miniature schnauzer	Small
SA027997	325103	Diabetic	Neutered male	Fasted	Mixed	Large
SA027998	216422	Diabetic	Neutered male	Fed	Dachshund	Small
SA027999	309443	Diabetic	Neutered male	Fed	Mixed	Medium
SA028000	321588	Diabetic	Spayed female	Fasted	Dachshund	Small
SA028001	325942	Diabetic	Spayed female	Fasted	Dachshund	Small
SA028002	213267	Diabetic	Spayed female	Fasted	Labrador retriever	Large
SA028003	279436	Diabetic	Spayed female	Fed	Labrador retriever	Large
SA028004	325643	Diabetic	Spayed female	Fed	Labrador retriever	Large
SA028005	269031	Diabetic	Spayed female	Fed	Mixed	Small
SA028006	323294	Diabetic	Spayed female	Fed	Shih Tzu	Small
SA028007	290374	Healthy	Neutered male	Fasted	Flat coated retriever	Large
SA028008	255293	Healthy	Neutered male	Fasted	Labrador retriever	Large
SA028009	223169	Healthy	Neutered male	Fed	Dachshund	Small
SA028010	290195	Healthy	Neutered male	Fed	Labrador retriever	Large
SA028011	325932	Healthy	Neutered male	Fed	Mixed	Medium
SA028012	296735	Healthy	Neutered male	Fed	Mixed	Small
SA028013	326077	Healthy	Neutered male	Fed	Shih Tzu	Small
SA028014	325866	Healthy	Spayed female	Fasted	Dachshund	Small
SA028015	288261	Healthy	Spayed female	Fasted	Dachshund	Small
SA028016	228415	Healthy	Spayed female	Fasted	Labrador retriever	Large
SA028017	295029	Healthy	Spayed female	Fasted	Miniature schnauzer	Small
SA028018	287971	Healthy	Spayed female	Fed	Labrador retriever	Large

Showing results 1 to 24 of 24

Showing results 1 to 24 of 24

Collection:

Collection ID:	CO000557
Collection Summary:	Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C.
Sample Type:	Blood (serum)
Collection Method:	Peripheral venipuncture
Collection Location:	Jugular, saphenous, or cephalic vein
Collection Frequency:	Once
Storage Conditions:	Room temperature until centrifugation. Then -80 C immediately after separation from red cells. Serum was thawed once on ice, 100 uL aliquots taken, and aliquots re-frozen (at -80C) until use for metabolomics.
Collection Vials:	Red top with clot activator
Storage Vials:	Cryovials (polypropylene with silicone gasket - Fisher Brand)
Collection Tube Temp:	Room temperature
Blood Serum Or Plasma:	Serum

Treatment:

Treatment ID:	TR000577
Treatment Summary:	None

Sample Preparation:

Sampleprep ID:	SP000570
Sampleprep Summary:	See protocol
Sampleprep Protocol Filename:	Metabolomics_LCMSProtocol_plasma.pdf

Chromatography:

Chromatography ID:	CH000587
Chromatography Summary:	see protocol
Instrument Name:	Thermo Scientific Q Exactive
Column Name:	ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:	Reversed phase

Analysis:

Analysis ID:	AN000822
Analysis Type:	MS
Instrument Name:	Q Exactive
Chromatography ID:	CH000587
Num Factors:	21
Num Metabolites:	127
Rt Units:	Minutes
Units:	Peak Area

Analysis ID:	AN000823
Analysis Type:	MS
Instrument Name:	Q Exactive
Chromatography ID:	CH000587
Num Factors:	21
Num Metabolites:	117
Rt Units:	Minutes
Units:	Peak Area