Summary of Study ST000554
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000406. The data can be accessed directly via it's Project DOI: 10.21228/M8K89B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000554 |
| Study Title | Metabolomics of aged iPSC |
| Study Type | baseline |
| Study Summary | The aim of the study is to perform global metabolomics of aged iPSC. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Memorial Sloan Kettering Institute |
| Last Name | Dogan |
| First Name | Yildirim |
| Address | 417 E 68th St. New York NY 10021 |
| dogany@mskcc.org | |
| Phone | 646-888-3578 |
| Submit Date | 2017-02-01 |
| Num Groups | 7 |
| Total Subjects | 16 |
| Study Comments | Groups: ESC / YiPSC / AiPSC / AiPSC_glut3 / AiPSC_ZSCAN10/ YiPSC_DCA/YiPSC_Citr |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000406 |
| Project DOI: | doi: 10.21228/M8K89B |
| Project Title: | Metabolomics of aged iPSC |
| Project Summary: | The aim of the study is to perform global metabolomics of aged iPSC. |
| Institute: | Memorial Sloan Kettering Institute |
| Department: | Cancer Biology |
| Last Name: | Kim |
| First Name: | Kitai |
| Address: | 417 E 68th St. New York NY 10021 |
| Email: | kimk@mskcc.org |
| Phone: | 646-888-3578 |
Subject:
| Subject ID: | SU000576 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | B6/CBA |
| Cell Primary Immortalized: | B6/CBA |
| Cell Passage Number: | Cultured primary cells |
| Cell Counts: | 16-18 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA028733 | kim_YiPSC_DCA_11 | 5mM Disodium acetate |
| SA028734 | kim_YiPSC_DCA_14 | 5mM Disodium acetate |
| SA028735 | kim_YiPSC_DCA_13 | 5mM Disodium acetate |
| SA028736 | kim_YiPSC_DCA_4 | 5mM Disodium acetate |
| SA028737 | kim_YiPSC_Citr_25 | 6mM Sodium Citrate |
| SA028738 | kim_YiPSC_Citr_39 | 6mM Sodium Citrate |
| SA028739 | kim_AiPSC_ZSCAN10_7 | Untreated |
| SA028740 | kim_AiPSC_ZSCAN10_19 | Untreated |
| SA028741 | kim_YiPSC_1-8 | Untreated |
| SA028742 | kim_YiPSC_1-3 | Untreated |
| SA028743 | kim_ESC-H | Untreated |
| SA028744 | kim_AiPSC_16 | Untreated |
| SA028745 | kim_AiPSC_19 | Untreated |
| SA028746 | kim_AiPSC_glut3_gcn4 | Untreated |
| SA028747 | kim_AiPSC_glut3_gcn3 | Untreated |
| SA028748 | kim_ESC-E | Untreated |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000570 |
| Collection Summary: | none |
| Collection Protocol Comments: | Cells were scraped off on ice in pbs |
| Sample Type: | Cultured primary cells |
| Collection Location: | Cold Room |
| Collection Frequency: | two plates at a time |
| Collection Duration: | 15 mins |
| Volumeoramount Collected: | In 5ml cold PBS |
| Storage Conditions: | N2 snap frozen, stored at -80 |
| Collection Vials: | Falcon tube and eppendorf tube |
| Storage Vials: | Eppendorf tube |
| Collection Tube Temp: | <4C |
| Additives: | None |
| Tissue Cell Quantity Taken: | 5-10mill |
Treatment:
| Treatment ID: | TR000590 |
| Treatment Summary: | No treatment |
| Plant Humidity: | - |
Sample Preparation:
| Sampleprep ID: | SP000583 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf |
Combined analysis:
| Analysis ID | AN000849 | AN000850 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | None | None |
| Column | None | None |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000606 |
| Methods Filename: | Metabolomics_Global_LCMS.pdf |
| Instrument Name: | None |
| Column Name: | None |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000750 |
| Analysis ID: | AN000849 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000751 |
| Analysis ID: | AN000850 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI |
| Ion Mode: | NEGATIVE |
