Summary of Study ST000555

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000375. The data can be accessed directly via it's Project DOI: 10.21228/M8KC7M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000555
Study TitleCocaine administration and withdrawal (part II)
Study Typetreatment and control
Study SummaryThe goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to cocaine administration and withdrawal as compared to control
Institute
University of Florida
DepartmentSECIM
LaboratoryBiochemistry and Molecular Biology
Last NameDownes
First NameDaniel
Address1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611
Emaildaniel.downes@neurosurgery.ufl.edu
Phone321-303-6523
Submit Date2017-01-23
Num Groups5
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Daniel Downes Daniel Downes
https://dx.doi.org/10.21228/M8KC7M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000375
Project DOI:doi: 10.21228/M8KC7M
Project Title:Drug's effect on cerebral metabolic pool sizes
Project Summary:The goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to drug administration and withdrawal
Institute:University of Florida
Department:Biochemistry and Molecular Biology
Last Name:Joanna
First Name:Long
Address:1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611
Email:jrlong@mbi.ufl.edu
Phone:none
Funding Source:UF

Subject:

Subject ID:SU000577
Subject Type:Long Evans Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Long Evans Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id treatment
SA028749LongDNP_001acute
SA028750LongDNP_003acute
SA028751LongDNP_002acute
SA028752LongDNP_006acute control
SA028753LongDNP_004acute control
SA028754LongDNP_005acute control
SA028755LongDNP_009withdrawal
SA028756LongDNP_007withdrawal
SA028757LongDNP_008withdrawal
SA028758LongDNP_012withdrawal control
SA028759LongDNP_011withdrawal control
SA028760LongDNP_010withdrawal control
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000571
Collection Summary:harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2
Sample Type:Tissue

Treatment:

Treatment ID:TR000591
Treatment Summary:Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal

Sample Preparation:

Sampleprep ID:SP000584
Sampleprep Summary:none
Sampleprep Protocol Filename:Metabolomics_Global_LCMS.pdf
Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000851 AN000852
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000607
Methods Filename:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000752
Analysis ID:AN000851
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000753
Analysis ID:AN000852
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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