Summary of Study ST000556
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000407. The data can be accessed directly via it's Project DOI: 10.21228/M8FG7D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000556 |
| Study Title | IROA feasibility project; plasticizers as obesogens in zebrafish |
| Study Type | (1) IROA label (2) Zebrafish exposed to DEHP |
| Study Summary | Zebrafish were fed IROA labelled nematodes (smaple 1-4); In a second experiment, zebrafish larvae were exposed to DEHP, a chemical that is a suspected obesogen. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Martyniuk |
| First Name | Chris |
| Address | 2187 Mowry Rd. Bldg 471 |
| cmartyn@ufl.edu | |
| Phone | 352-294-4636 |
| Submit Date | 2017-01-26 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Study Comments | Expt 2: Two groups (control) + DEHP-treated (n=10 larvae pools or biological replicates) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000407 |
| Project DOI: | doi: 10.21228/M8FG7D |
| Project Title: | IROA feasibility project; plasticizers as obesogens in zebrafish |
| Project Summary: | IROA feasibility project; plasticizers as obesogens in zebrafish |
| Institute: | University of Florida |
| Department: | Physiological Sciences |
| Last Name: | Martyniuk |
| First Name: | Chris |
| Address: | 2187 Mowry Rd. Bldg 471 |
| Email: | cmartyn@ufl.edu |
| Phone: | 352-294-4636 |
| Funding Source: | "Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209 " |
Subject:
| Subject ID: | SU000578 |
| Subject Type: | Larval fish at one month |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Species Group: | Fish |
Factors:
Subject type: Larval fish at one month; Subject species: Danio rerio (Factor headings shown in green)
| mb_sample_id | local_sample_id | Chemical Treatment |
|---|---|---|
| SA028761 | M18A | DEHP |
| SA028762 | M20A | DEHP |
| SA028763 | M1A | DEHP |
| SA028764 | M12A | DEHP |
| SA028765 | M15A | DEHP |
| SA028766 | M11A | DEHP |
| SA028767 | M2A | DEHP |
| SA028768 | M5A | DEHP |
| SA028769 | M3A | DEHP |
| SA028770 | M7A | DEHP |
| SA028771 | C13A | DMSO |
| SA028772 | C15A | DMSO |
| SA028773 | C18A | DMSO |
| SA028774 | C12A | DMSO |
| SA028775 | C2A | DMSO |
| SA028776 | C3A | DMSO |
| SA028777 | C4A | DMSO |
| SA028778 | C5A | DMSO |
| SA028779 | C9A | DMSO |
| SA028780 | C10A | DMSO |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO000572 |
| Collection Summary: | Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. Fish were pooled into groups of 5 (10 biological replicates derived from 10 independent technical replicates. |
| Sample Type: | Whole larval fish. |
Treatment:
| Treatment ID: | TR000592 |
| Treatment Summary: | Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. |
| Treatment Compound: | DEHP (a plastic) |
| Treatment Route: | waterborne |
| Treatment Dose: | 10 uM DEHP |
| Treatment Doseduration: | 96 hours |
| Treatment Vehicle: | DMSO |
| Animal Vet Treatments: | None |
| Animal Anesthesia: | Tricaine |
| Animal Fasting: | Early stage larval fish, do not feed for first 144 hours. |
| Animal Endp Euthanasia: | MS-222 or Tricaine |
| Animal Endp Tissue Coll List: | Whole larval fish placed into a vial. Five fish per vial. |
| Animal Endp Clinical Signs: | No clinical signs |
Sample Preparation:
| Sampleprep ID: | SP000585 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000853 | AN000854 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000608 |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000754 |
| Analysis ID: | AN000853 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_Global_LCMS.pdf |
| MS ID: | MS000755 |
| Analysis ID: | AN000854 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_Global_LCMS.pdf |
