Summary of Study ST000564
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000232. The data can be accessed directly via it's Project DOI: 10.21228/M8P01G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000564 |
| Study Title | 2014 Biotron Experiment Metabolites (part II) |
| Study Type | Time course |
| Study Summary | Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Stewart |
| Last Name | Stewart |
| First Name | Jon |
| Address | 102 Leigh Hall, University of Florida |
| jds2@chem.ufl.edu | |
| Phone | 352-846-0743 |
| Num Groups | 1 |
| Total Subjects | 6 |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-07-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000232 |
| Project DOI: | doi: 10.21228/M8P01G |
| Project Title: | Climate Change and Starch Yield in Maize Part 2 |
| Project Type: | MS study |
| Project Summary: | Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination. |
| Institute: | University of Florida |
| Department: | Chemistry |
| Laboratory: | Stewart |
| Last Name: | Stewart |
| First Name: | Jon |
| Address: | 126 Sisler Hall |
| Email: | jds2@chem.ufl.edu |
| Phone: | 846-0743 |
| Funding Source: | USDA |
Subject:
| Subject ID: | SU000586 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Species Group: | Plant |
Factors:
Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)
| mb_sample_id | local_sample_id | Time |
|---|---|---|
| SA029060 | Control 14 DAP | 14 days after pollination |
| SA029061 | Control 16 DAP | 16 days after pollination |
| SA029062 | Control 18 DAP | 18 days after pollination |
| SA029063 | Control 22 DAP | 22 days after pollination |
| SA029064 | Control 26 DAP | 26 days after pollination |
| SA029065 | Control 40 DAP | 40 days after pollination |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO000580 |
| Collection Summary: | None |
| Sample Type: | Maize kernel |
| Collection Method: | Manual removal from ear |
| Collection Location: | University of Wisconsin Biotron |
| Storage Conditions: | Frozen at -80C |
Treatment:
| Treatment ID: | TR000600 |
| Treatment Summary: | None |
| Plant Growth Location: | Wisconsin Biotron Facility |
| Plant Temp: | 28 C day / 17 C night |
| Plant Metab Quench Method: | Frozen at -80C |
Sample Preparation:
| Sampleprep ID: | SP000593 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Maize_Tissue_sample_prep.pdf |
Combined analysis:
| Analysis ID | AN000866 | AN000867 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH000616 |
| Chromatography Summary: | None |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000767 |
| Analysis ID: | AN000866 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000768 |
| Analysis ID: | AN000867 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
