Summary of Study ST000565

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000415. The data can be accessed directly via it's Project DOI: 10.21228/M8DK6R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000565
Study TitleAssociation of hemodialysis patient plasma trace metals with response to erythropoiesis stimulating agents
Study TypeMetallomics
Study SummaryEDTA-Plasma from 110 hemodialysis patients participating in an NIDDK funded study were analyzed by ICP-MS for the concentration of As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Sn, V, and Zn. Associations were determined between trace metals and gender, race, hemodialysis status, hemoglobin at the time of draw (Hgb), total ESA dose for the month the sample was collected (EPO), and erythropoietin resistance index determined over the 6 months of treatment leading up to sample collection (ERI)
Institute
RTI International
LaboratoryNIH Eastern Regional Comprehensive Metabolomics Resource Core at UNC Chapel Hill (ERCMRC)
Last NameSumner
First NameSusan
Address3040 E. Cornwallis Road, Research Triangle Park, NC 27709, USA
Emailsusan_sumner@unc.edu
Phone704-250-5000
Submit Date2017-02-27
Total Subjects110
Raw Data AvailableYes
Raw Data File Type(s)dat
Analysis Type DetailICP-MS
Release Date2018-08-27
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8DK6R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000415
Project DOI:doi: 10.21228/M8DK6R
Project Title:Association of hemodialysis patient plasma trace metals with response to erythropoiesis stimulating agents
Project Type:Metallomics
Project Summary:Erythropoietin (EPO) is a cytokine used to treat the anemia of chronic renal disease in greater than 90% of all in-center hemodialysis patients at a cost of approximately 2 billion dollars per year (2010). Despite protocols for anemia management in the end stage kidney disease (ESKD) population, a large proportion of patients do not predictably respond to typical doses of EPO. Several recent randomized controlled trials looking to increase hemoglobin in patients with the anemia of renal disease have uncovered many questions about the treatment of anemia with erythropoietic stimulating agents (ESA) not previously addressed in new drug applications or in subsequent research. We sought to test the hypothesis that serum trace metal concentrations correlate with markers of anemia and response to ESA treatment. EDTA-Plasma from 110 hemodialysis patients participating in an NIDDK funded study (R01-01DK091584) were analyzed by ICP-MS for the concentration of As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Sn, V, and Zn. Associations were determined between trace metals and gender, race, hemodialysis status, hemoglobin at the time of draw (Hgb), total ESA dose for the month the sample was collected (EPO), and erythropoietin resistance index determined over the 6 months of treatment leading up to sample collection (ERI).
Institute:University of Louisville
Department:School of Medicine, Division of Nephrology & Hypertension
Last Name:Merchant
First Name:Michael
Address:550 South Jackson Street, 3rd Floor, Louisville, Kentucky 40202
Email:michael.merchant@louisville.edu
Phone:502/852-0245
Funding Source:NIH Grants 1R01DK091584, U24DK097193, and K01GM109320

Subject:

Subject ID:SU000587
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Both
Human Trial Type:validation/discovery trial
Human Inclusion Criteria:All subjects will receive hemodialysis in-center on a 3 times per week dialysis schedule. Subjects are eligible to participate if 1) subjects that have received EPO will have received it for 6 or more months, 2) between the ages of 18 and 80 years, 3) have an adequacy of dialysis (Kt/V) that is ≥ 1.2, 4) have adequate iron stores as measured by ferritin and transferrin saturation.
Human Exclusion Criteria:None
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Gender Race
SA029066M87F -
SA029067M76F African American
SA029068M33F African American
SA029069M7F African American
SA029070M72F African American
SA029071M45F Caucasian
SA029072M44F Caucasian
SA029073M1F Caucasian
SA029074M52F Caucasian
SA029075M55F Caucasian
SA029076M4F Caucasian
SA029077M39F Caucasian
SA029078M98F Caucasian
SA029079M3F Caucasian
SA029080M34F Caucasian
SA029081M35F Caucasian
SA029082M38F Caucasian
SA029083M58F Caucasian
SA029084M61F Caucasian
SA029085M81F Caucasian
SA029086M80F Caucasian
SA029087M84F Caucasian
SA029088M85F Caucasian
SA029089M92F Caucasian
SA029090M78F Caucasian
SA029091M77F Caucasian
SA029092M95F Caucasian
SA029093M65F Caucasian
SA029094M26F Caucasian
SA029095M74F Caucasian
SA029096M94F Caucasian
SA029097M91F Caucasian
SA029098M47F Caucasian
SA029099M106F Caucasian
SA029100M107F Caucasian
SA029101M105F Caucasian
SA029102M15F Caucasian
SA029103M28F Other
SA029104M86F Other
SA029105M114F Other
SA029106M101F Other
SA029107M82M African American
SA029108M48M African American
SA029109M16M African American
SA029110M57M African American
SA029111M75M African American
SA029112M110M African American
SA029113M8M African American
SA029114M19M African American
SA029115M24M African American
SA029116M32M African American
SA029117M100M African American
SA029118M73M Caucasian
SA029119M96M Caucasian
SA029120M112M Caucasian
SA029121M97M Caucasian
SA029122M71M Caucasian
SA029123M67M Caucasian
SA029124M68M Caucasian
SA029125M115M Caucasian
SA029126M70M Caucasian
SA029127M111M Caucasian
SA029128M104M Caucasian
SA029129M66M Caucasian
SA029130M83M Caucasian
SA029131M90M Caucasian
SA029132M109M Caucasian
SA029133M11M Caucasian
SA029134M102M Caucasian
SA029135M79M Caucasian
SA029136M93M Caucasian
SA029137M103M Caucasian
SA029138M88M Caucasian
SA029139M64M Caucasian
SA029140M46M Caucasian
SA029141M12M Caucasian
SA029142M17M Caucasian
SA029143M49M Caucasian
SA029144M5M Caucasian
SA029145M31M Caucasian
SA029146M42M Caucasian
SA029147M22M Caucasian
SA029148M2M Caucasian
SA029149M40M Caucasian
SA029150M41M Caucasian
SA029151M37M Caucasian
SA029152M50M Caucasian
SA029153M25M Caucasian
SA029154M29M Caucasian
SA029155M63M Caucasian
SA029156M27M Caucasian
SA029157M51M Caucasian
SA029158M6M Caucasian
SA029159M54M Caucasian
SA029160M14M Caucasian
SA029161M30M Caucasian
SA029162M53M Caucasian
SA029163M56M Caucasian
SA029164M36M Other
SA029165M10M Other
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Collection:

Collection ID:CO000581
Collection Summary:Subjects that passed the screening were asked to donate 20 mL of blood for analysis following an informed consent process. The blood was processed to plasma and serum and stored as 0.5mL aliquots at -70 degrees until analyzed.
Sample Type:Blood
Additives:EDTA
Blood Serum Or Plasma:Plasma

Treatment:

Treatment ID:TR000601
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP000594
Sampleprep Summary:A volume of 150 µL of each EDTA-plasma sample was dispensed into an acid-washed plastic digestion tube. To each digestion vessel, 300 µL of concentrated HNO3, 150 µL of concentrated HCl, and 100 µL of H2O2 solution was added. Samples were loosely capped and placed in the graphite digestion block and heated at a temperature of 90 °C for 2 hours. At the end of digestion, all samples were removed from the heating block and allowed to cool to room temperature. In the clean hood, samples were spiked with a multielement internal standard to provide a final concentration of 1.0 ng/mL Sc, In, and Pr, and diluted to the final volume with deionized (DI) H2O.
Processing Method:Acid Digestion
Processing Storage Conditions:90°C
Extract Storage:8°C
Sample Spiking:Sc, In, Pr

Combined analysis:

Analysis ID AN000868
Analysis type MS
Chromatography type ICP
Chromatography system Thermo Element 2 ICP-MS
Column None
MS Type Other
MS instrument type Sector Field
MS instrument name Thermo Element 2 ICP
Ion Mode POSITIVE
Units ng/mL plasma

Chromatography:

Chromatography ID:CH000617
Instrument Name:Thermo Element 2 ICP-MS
Column Name:None
Chromatography Type:ICP

MS:

MS ID:MS000769
Analysis ID:AN000868
Instrument Name:Thermo Element 2 ICP
Instrument Type:Sector Field
MS Type:Other
Ion Mode:POSITIVE
Mass Accuracy:7000 (medium resolution) and 10000 (high resolution)
Dataformat:.dat
Scan Range Moverz:27-209
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