Summary of Study ST000566

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000387. The data can be accessed directly via it's Project DOI: 10.21228/M81G7P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000566
Study TitleLarge Scale C18 Profiling of the Effects of Curcumin Supplementation of Older Adults: Relation to Vascular Function (part 5)
Study SummaryPerform large scale profiling C18 metabolite analysis related to nitric oxide biology, oxidative stress and inflammation in plasma before and after 12 weeks of oral curcumin (2000 mg/d) or placebo (double-blind, randomized) in men and women aged 45-79 years who are free from clinical cardiovascular disease.
Institute
Mayo Clinic
Last NameSeals
First NameDouglas
AddressDepartment of Integrative Physiology University of Colorado Boulder, CO 80309
Emailseals@colorado.edu
Phone303-492-5305
Submit Date2017-03-03
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2019-03-06
Release Version1
Douglas Seals Douglas Seals
https://dx.doi.org/10.21228/M81G7P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000387
Project DOI:doi: 10.21228/M81G7P
Project Title:Mayo Metabolomics Pilot and Feasibility Award: Effects of Curcumin Supplementation on the Plasma Metabolome of Older Adults: Relation to Vascular Function
Project Summary:Age is the major risk factor for cardiovascular diseases (CVD). Two key contributors to the increased risk of CVD in middle-aged and older (MA/O) adults are stiffening of the large elastic arteries and the development of vascular endothelial dysfunction, indicated by impaired nitric oxide (NO)-induced endothelium-dependent dilation (EDD). The mechanisms by which aging causes arterial dysfunction are incompletely understood, but involve reductions in NO bioavailability associated with the development of oxidative stress and inflammation. Thus, establishing novel strategies to reduce arterial stiffness and increase vascular endothelial function in MA/O adults by increasing NO bioavailability and reducing oxidative stress and inflammation are a high biomedical research priority. Curcumin is a naturally occurring phenol found in the Indian spice turmeric that improves physiological function in animal models of age-related diseases and is a promising nutraceutical for intervention for promoting healthy aging. Our preclinical results indicate that chow supplemented with curcumin reduces aortic pulse wave velocity (PWV), the most common and clinically important measure of large elastic artery stiffness, restores NO-mediated EDD and reduces arterial oxidative stress and inflammation in old C57/BL6 mice. Preliminary data from our recently funded NIH R21 pilot grant indicate that curcumin supplementation improves vascular function in humans. It is possible that changes in the circulating (plasma) metabolome with oral curcumin supplementation will provide insight into novel metabolic mechanisms by which curcumin may improve vascular function. The goal of this project is to identify key metabolic pathways that change with oral curcumin supplementation and to relate those changes with improvements in vascular function in MA/O adults with initial endothelial dysfunction. Metabolomic analysis of plasma samples at baseline also may produce unique molecular signatures that predict responsiveness (changes in vascular function) to curcumin supplementation among individuals.
Institute:Mayo Clinic
Last Name:Seals
First Name:Douglas
Address:Department of Integrative Physiology University of Colorado Boulder, CO 80309
Email:seals@colorado.edu
Phone:303-492-5305

Subject:

Subject ID:SU000588
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id group Time point
SA02917828may15_011-r001Old-Curcumin Post
SA02917928may15_032-r001Old-Curcumin Post
SA02918004jun15_032-r001Old-Curcumin Post
SA02918126may15_011-r001Old-Curcumin Post
SA02918226may15_032-r001Old-Curcumin Post
SA02918328may15_006-r001Old-Curcumin Post
SA02918426may15_025-r001Old-Curcumin Post
SA02918504jun15_018-r001Old-Curcumin Post
SA02918628may15_030-r001Old-Curcumin Post
SA02918728may15_023-r001Old-Curcumin Post
SA02918828may15_021-r001Old-Curcumin Post
SA02918928may15_013-r001Old-Curcumin Post
SA02919004jun15_025-r001Old-Curcumin Post
SA02919126may15_023-r001Old-Curcumin Post
SA02919226may15_013-r001Old-Curcumin Post
SA02919328may15_025-r001Old-Curcumin Post
SA02919401jun15_006-r001Old-Curcumin Post
SA02919528may15_018-r001Old-Curcumin Post
SA02919604jun15_030-r001Old-Curcumin Post
SA02919701jun15_028-r001Old-Curcumin Post
SA02919804jun15_008-r001Old-Curcumin Post
SA02919901jun15_030-r001Old-Curcumin Post
SA02920004jun15_006-r001Old-Curcumin Post
SA02920101jun15_032-r001Old-Curcumin Post
SA02920226may15_021-r001Old-Curcumin Post
SA02920326may15_030-r001Old-Curcumin Post
SA02920401jun15_025-r001Old-Curcumin Post
SA02920526may15_006-r001Old-Curcumin Post
SA02920601jun15_008-r001Old-Curcumin Post
SA02920726may15_018-r001Old-Curcumin Post
SA02920801jun15_018-r001Old-Curcumin Post
SA02920904jun15_028-r001Old-Curcumin Post
SA02921028may15_013-r002Old-Curcumin Post
SA02921128may15_006-r002Old-Curcumin Post
SA02921226may15_030-r002Old-Curcumin Post
SA02921328may15_011-r002Old-Curcumin Post
SA02921404jun15_032-r002Old-Curcumin Post
SA02921526may15_018-r002Old-Curcumin Post
SA02921628may15_030-r002Old-Curcumin Post
SA02921701jun15_018-r002Old-Curcumin Post
SA02921826may15_006-r002Old-Curcumin Post
SA02921901jun15_025-r002Old-Curcumin Post
SA02922001jun15_028-r002Old-Curcumin Post
SA02922101jun15_032-r002Old-Curcumin Post
SA02922201jun15_030-r002Old-Curcumin Post
SA02922301jun15_008-r002Old-Curcumin Post
SA02922401jun15_006-r002Old-Curcumin Post
SA02922528may15_023-r002Old-Curcumin Post
SA02922626may15_013-r002Old-Curcumin Post
SA02922728may15_025-r002Old-Curcumin Post
SA02922828may15_032-r002Old-Curcumin Post
SA02922926may15_032-r002Old-Curcumin Post
SA02923026may15_011-r002Old-Curcumin Post
SA02923128may15_018-r002Old-Curcumin Post
SA02923228may15_021-r002Old-Curcumin Post
SA02923326may15_021-r002Old-Curcumin Post
SA02923404jun15_025-r002Old-Curcumin Post
SA02923504jun15_028-r002Old-Curcumin Post
SA02923604jun15_008-r002Old-Curcumin Post
SA02923726may15_025-r002Old-Curcumin Post
SA02923826may15_023-r002Old-Curcumin Post
SA02923904jun15_030-r002Old-Curcumin Post
SA02924004jun15_018-r002Old-Curcumin Post
SA02924104jun15_006-r002Old-Curcumin Post
SA02924228may15_020-r001Old-Curcumin Pre
SA02924328may15_012-r001Old-Curcumin Pre
SA02924401jun15_024-r001Old-Curcumin Pre
SA02924526may15_022-r001Old-Curcumin Pre
SA02924628may15_010-r001Old-Curcumin Pre
SA02924701jun15_027-r001Old-Curcumin Pre
SA02924804jun15_017-r001Old-Curcumin Pre
SA02924928may15_031-r001Old-Curcumin Pre
SA02925028may15_017-r001Old-Curcumin Pre
SA02925128may15_029-r001Old-Curcumin Pre
SA02925226may15_031-r001Old-Curcumin Pre
SA02925326may15_012-r001Old-Curcumin Pre
SA02925404jun15_005-r001Old-Curcumin Pre
SA02925501jun15_031-r001Old-Curcumin Pre
SA02925604jun15_024-r001Old-Curcumin Pre
SA02925726may15_017-r001Old-Curcumin Pre
SA02925828may15_024-r001Old-Curcumin Pre
SA02925901jun15_007-r001Old-Curcumin Pre
SA02926026may15_024-r001Old-Curcumin Pre
SA02926126may15_020-r001Old-Curcumin Pre
SA02926201jun15_017-r001Old-Curcumin Pre
SA02926304jun15_031-r001Old-Curcumin Pre
SA02926426may15_029-r001Old-Curcumin Pre
SA02926501jun15_005-r001Old-Curcumin Pre
SA02926604jun15_029-r001Old-Curcumin Pre
SA02926704jun15_027-r001Old-Curcumin Pre
SA02926826may15_005-r001Old-Curcumin Pre
SA02926904jun15_007-r001Old-Curcumin Pre
SA02927001jun15_029-r001Old-Curcumin Pre
SA02927126may15_010-r001Old-Curcumin Pre
SA02927228may15_005-r001Old-Curcumin Pre
SA02927328may15_022-r001Old-Curcumin Pre
SA02927426may15_012-r002Old-Curcumin Pre
SA02927528may15_029-r002Old-Curcumin Pre
SA02927601jun15_024-r002Old-Curcumin Pre
SA02927701jun15_005-r002Old-Curcumin Pre
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Collection:

Collection ID:CO000582
Collection Summary:Healthy men & women aged 45-79 without clinical CVD, but with below normal baseline endothelial dysfunction (FMDBA < 7%).
Sample Type:Blood

Treatment:

Treatment ID:TR000602
Treatment Summary:A 12-week randomized, double-blind, placebo controlled study will be conducted. After CTRC screening for inclusion/exclusion criteria, qualified subjects will be randomly assigned to 1 of 2 groups. The investigators involved in the acquisition and analysis of key outcomes will be blinded to the curcumin intake status of the subjects. With the assistance of dietary monitoring from the UC-Boulder CTRC bionutritionists, subjects will maintain their baseline diet with either unchanged (control) or enhanced curcumin intake delivered as capsules (Longvida®, Verdure Sciences): Group 1 = placebo (inert substances); Group 2 = curcumin (2000mg curcumin/day). Extensive published work has established that the curcumin dose of 2000 mg/day is well tolerated and safe. Sessions 1 & 2: Screening measurements. Session 3: Baseline measurements and blood draw. Sessions 4-8 (every other week to assess adherence and overall subject well-being): Body weight, BP, adherence, discuss any problems. Session 9: Identical to session 3 (stop intake of capsules after completion of post-testing).

Sample Preparation:

Sampleprep ID:SP000595
Sampleprep Summary:Venous blood sampling. All blood samples are handled in a similar time frame and immediately spun to extract plasma and stored in our -80 freezer until ready for analysis.

Combined analysis:

Analysis ID AN000869 AN000870
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS C18 (150 x 2.1mm,1.8um) Waters Acquity HSS C18 (150 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6550 QTOF Agilent 6550 QTOF
Ion Mode NEGATIVE POSITIVE
Units intensity intensity

Chromatography:

Chromatography ID:CH000618
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS C18 (150 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000770
Analysis ID:AN000869
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000771
Analysis ID:AN000870
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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