Summary of Study ST000567

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000387. The data can be accessed directly via it's Project DOI: 10.21228/M81G7P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000567
Study TitleLarge Scale HILIC Profiling of the Effects of Curcumin Supplementation of Older Adults: Relation to Vascular Function (part 6)
Study SummaryPerform large scale profiling HILIC metabolite analysis related to nitric oxide biology, oxidative stress and inflammation in plasma before and after 12 weeks of oral curcumin (2000 mg/d) or placebo (double-blind, randomized) in men and women aged 45-79 years who are free from clinical cardiovascular disease.
Institute
Mayo Clinic
Last NameSeals
First NameDouglas
AddressDepartment of Integrative Physiology University of Colorado Boulder, CO 80309
Emailseals@colorado.edu
Phone303-492-5305
Submit Date2017-03-03
Analysis Type DetailLC-MS
Release Date2019-03-06
Release Version1
Douglas Seals Douglas Seals
https://dx.doi.org/10.21228/M81G7P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000387
Project DOI:doi: 10.21228/M81G7P
Project Title:Mayo Metabolomics Pilot and Feasibility Award: Effects of Curcumin Supplementation on the Plasma Metabolome of Older Adults: Relation to Vascular Function
Project Summary:Age is the major risk factor for cardiovascular diseases (CVD). Two key contributors to the increased risk of CVD in middle-aged and older (MA/O) adults are stiffening of the large elastic arteries and the development of vascular endothelial dysfunction, indicated by impaired nitric oxide (NO)-induced endothelium-dependent dilation (EDD). The mechanisms by which aging causes arterial dysfunction are incompletely understood, but involve reductions in NO bioavailability associated with the development of oxidative stress and inflammation. Thus, establishing novel strategies to reduce arterial stiffness and increase vascular endothelial function in MA/O adults by increasing NO bioavailability and reducing oxidative stress and inflammation are a high biomedical research priority. Curcumin is a naturally occurring phenol found in the Indian spice turmeric that improves physiological function in animal models of age-related diseases and is a promising nutraceutical for intervention for promoting healthy aging. Our preclinical results indicate that chow supplemented with curcumin reduces aortic pulse wave velocity (PWV), the most common and clinically important measure of large elastic artery stiffness, restores NO-mediated EDD and reduces arterial oxidative stress and inflammation in old C57/BL6 mice. Preliminary data from our recently funded NIH R21 pilot grant indicate that curcumin supplementation improves vascular function in humans. It is possible that changes in the circulating (plasma) metabolome with oral curcumin supplementation will provide insight into novel metabolic mechanisms by which curcumin may improve vascular function. The goal of this project is to identify key metabolic pathways that change with oral curcumin supplementation and to relate those changes with improvements in vascular function in MA/O adults with initial endothelial dysfunction. Metabolomic analysis of plasma samples at baseline also may produce unique molecular signatures that predict responsiveness (changes in vascular function) to curcumin supplementation among individuals.
Institute:Mayo Clinic
Last Name:Seals
First Name:Douglas
Address:Department of Integrative Physiology University of Colorado Boulder, CO 80309
Email:seals@colorado.edu
Phone:303-492-5305

Subject:

Subject ID:SU000589
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id groups Time point
SA02946613may15_013-r001Old-Curcumin Post
SA02946717may15_021-r001Old-Curcumin Post
SA02946813may15_011-r001Old-Curcumin Post
SA02946913may15_006-r001Old-Curcumin Post
SA02947013may15_018-r001Old-Curcumin Post
SA02947117may15_018-r001Old-Curcumin Post
SA02947213may15_025-r001Old-Curcumin Post
SA02947313may15_023-r001Old-Curcumin Post
SA02947417may15_032-r001Old-Curcumin Post
SA02947517may15_023-r001Old-Curcumin Post
SA02947613may15_030-r001Old-Curcumin Post
SA02947718may15_018-r001Old-Curcumin Post
SA02947818may15_008-r001Old-Curcumin Post
SA02947917may15_030-r001Old-Curcumin Post
SA02948018may15_025-r001Old-Curcumin Post
SA02948118may15_028-r001Old-Curcumin Post
SA02948218may15_032-r001Old-Curcumin Post
SA02948317may15_025-r001Old-Curcumin Post
SA02948418may15_030-r001Old-Curcumin Post
SA02948518may15_006-r001Old-Curcumin Post
SA02948613may15_021-r001Old-Curcumin Post
SA02948715may15_030-r001Old-Curcumin Post
SA02948815may15_006-r001Old-Curcumin Post
SA02948917may15_011-r001Old-Curcumin Post
SA02949015may15_018-r001Old-Curcumin Post
SA02949117may15_006-r001Old-Curcumin Post
SA02949215may15_025-r001Old-Curcumin Post
SA02949315may15_028-r001Old-Curcumin Post
SA02949415may15_032-r001Old-Curcumin Post
SA02949515may15_008-r001Old-Curcumin Post
SA02949613may15_032-r001Old-Curcumin Post
SA02949717may15_013-r001Old-Curcumin Post
SA02949818may15_018-r002Old-Curcumin Post
SA02949915may15_028-r002Old-Curcumin Post
SA02950018may15_025-r002Old-Curcumin Post
SA02950115may15_018-r002Old-Curcumin Post
SA02950218may15_008-r002Old-Curcumin Post
SA02950315may15_025-r002Old-Curcumin Post
SA02950417may15_032-r002Old-Curcumin Post
SA02950518may15_006-r002Old-Curcumin Post
SA02950613may15_030-r002Old-Curcumin Post
SA02950713may15_023-r002Old-Curcumin Post
SA02950818may15_028-r002Old-Curcumin Post
SA02950913may15_021-r002Old-Curcumin Post
SA02951013may15_032-r002Old-Curcumin Post
SA02951113may15_018-r002Old-Curcumin Post
SA02951213may15_011-r002Old-Curcumin Post
SA02951315may15_032-r002Old-Curcumin Post
SA02951413may15_006-r002Old-Curcumin Post
SA02951515may15_006-r002Old-Curcumin Post
SA02951618may15_030-r002Old-Curcumin Post
SA02951715may15_030-r002Old-Curcumin Post
SA02951815may15_008-r002Old-Curcumin Post
SA02951913may15_025-r002Old-Curcumin Post
SA02952013may15_013-r002Old-Curcumin Post
SA02952118may15_032-r002Old-Curcumin Post
SA02952217may15_011-r002Old-Curcumin Post
SA02952317may15_018-r002Old-Curcumin Post
SA02952417may15_013-r002Old-Curcumin Post
SA02952517may15_025-r002Old-Curcumin Post
SA02952617may15_006-r002Old-Curcumin Post
SA02952717may15_021-r002Old-Curcumin Post
SA02952817may15_030-r002Old-Curcumin Post
SA02952917may15_023-r002Old-Curcumin Post
SA02953013may15_029-r001Old-Curcumin Pre
SA02953118may15_029-r001Old-Curcumin Pre
SA02953213may15_031-r001Old-Curcumin Pre
SA02953313may15_005-r001Old-Curcumin Pre
SA02953417may15_029-r001Old-Curcumin Pre
SA02953513may15_024-r001Old-Curcumin Pre
SA02953617may15_022-r001Old-Curcumin Pre
SA02953718may15_017-r001Old-Curcumin Pre
SA02953817may15_020-r001Old-Curcumin Pre
SA02953913may15_012-r001Old-Curcumin Pre
SA02954013may15_017-r001Old-Curcumin Pre
SA02954113may15_020-r001Old-Curcumin Pre
SA02954217may15_017-r001Old-Curcumin Pre
SA02954317may15_012-r001Old-Curcumin Pre
SA02954413may15_010-r001Old-Curcumin Pre
SA02954513may15_022-r001Old-Curcumin Pre
SA02954618may15_007-r001Old-Curcumin Pre
SA02954715may15_029-r001Old-Curcumin Pre
SA02954818may15_031-r001Old-Curcumin Pre
SA02954915may15_007-r001Old-Curcumin Pre
SA02955015may15_017-r001Old-Curcumin Pre
SA02955115may15_024-r001Old-Curcumin Pre
SA02955217may15_005-r001Old-Curcumin Pre
SA02955318may15_027-r001Old-Curcumin Pre
SA02955417may15_024-r001Old-Curcumin Pre
SA02955517may15_010-r001Old-Curcumin Pre
SA02955615may15_027-r001Old-Curcumin Pre
SA02955718may15_005-r001Old-Curcumin Pre
SA02955817may15_031-r001Old-Curcumin Pre
SA02955915may15_005-r001Old-Curcumin Pre
SA02956015may15_031-r001Old-Curcumin Pre
SA02956118may15_024-r001Old-Curcumin Pre
SA02956217may15_031-r002Old-Curcumin Pre
SA02956318may15_029-r002Old-Curcumin Pre
SA02956418may15_031-r002Old-Curcumin Pre
SA02956517may15_024-r002Old-Curcumin Pre
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Collection:

Collection ID:CO000583
Collection Summary:Healthy men & women aged 45-79 without clinical CVD, but with below normal baseline endothelial dysfunction (FMDBA < 7%).
Sample Type:Blood

Treatment:

Treatment ID:TR000603
Treatment Summary:A 12-week randomized, double-blind, placebo controlled study will be conducted. After CTRC screening for inclusion/exclusion criteria, qualified subjects will be randomly assigned to 1 of 2 groups. The investigators involved in the acquisition and analysis of key outcomes will be blinded to the curcumin intake status of the subjects. With the assistance of dietary monitoring from the UC-Boulder CTRC bionutritionists, subjects will maintain their baseline diet with either unchanged (control) or enhanced curcumin intake delivered as capsules (Longvida®, Verdure Sciences): Group 1 = placebo (inert substances); Group 2 = curcumin (2000mg curcumin/day). Extensive published work has established that the curcumin dose of 2000 mg/day is well tolerated and safe. Sessions 1 & 2: Screening measurements. Session 3: Baseline measurements and blood draw. Sessions 4-8 (every other week to assess adherence and overall subject well-being): Body weight, BP, adherence, discuss any problems. Session 9: Identical to session 3 (stop intake of capsules after completion of post-testing).

Sample Preparation:

Sampleprep ID:SP000596
Sampleprep Summary:Venous blood sampling. All blood samples are handled in a similar time frame and immediately spun to extract plasma and stored in our -80 freezer until ready for analysis.

Combined analysis:

Analysis ID AN000871 AN000872
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6550 QTOF Agilent 6550 QTOF
Ion Mode NEGATIVE POSITIVE
Units intensity intensity

Chromatography:

Chromatography ID:CH000619
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS000772
Analysis ID:AN000871
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000773
Analysis ID:AN000872
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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