Summary of Study ST000571

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000419. The data can be accessed directly via it's Project DOI: 10.21228/M8WK63 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000571
Study TitleMetabolomics of uterine fluid of the cow
Study Typetime course
Study SummaryDay 0, 3, 5 and 7 after estrus
Institute
University of Florida
DepartmentSECIM
LaboratoryP. J. Hansen
Last NameMoss
First NameJames
Address2250 SW Shealy Dr., Gainesville FL 32611
Emaildushka@ufl.edu
Phone(352) 339-2237
Submit Date2017-01-13
Num Groups4
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
James Moss James Moss
https://dx.doi.org/10.21228/M8WK63
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000419
Project DOI:doi: 10.21228/M8WK63
Project Title:Metabolomics of uterine fluid of the cow
Project Type:Research
Project Summary:The goal is to determine cyclic changes in the metabolome of uterine fluid
Institute:University of Florida
Department:IFAS
Last Name:Hansen
First Name:Peter
Address:PO Box 110910
Email:Hansen@animal.ufl.edu
Phone:352-359-5753
Funding Source:USDA-NIFA

Subject:

Subject ID:SU000593
Subject Type:Animal
Subject Species:Bos taurus
Taxonomy ID:9913
Genotype Strain:Holstein
Age Or Age Range:Adult
Gender:Female
Animal Animal Supplier:University of Florida
Animal Housing:Pasture
Animal Light Cycle:Natural
Animal Feed:Pasture
Animal Water:Ad Lib
Animal Inclusion Criteria:Cyclic
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Bos taurus (Factor headings shown in green)

mb_sample_id local_sample_id Days after ovulation
SA029942PJH_001D0
SA029943PJH_004D0
SA029944PJH_003D0
SA029945PJH_002D0
SA029946PJH_007D3
SA029947PJH_008D3
SA029948PJH_006D3
SA029949PJH_005D3
SA029950PJH_011D5
SA029951PJH_010D5
SA029952PJH_009D5
SA029953PJH_015D7
SA029954PJH_012D7
SA029955PJH_013D7
SA029956PJH_014D7
Showing results 1 to 15 of 15

Collection:

Collection ID:CO000587
Collection Summary:Recover uterus at slaughter; flush uterine lumen with 30 ml DPBS
Sample Type:Uterine fluid
Collection Method:Flush
Volumeoramount Collected:30 ml
Storage Conditions:-20 C

Treatment:

Treatment ID:TR000607
Treatment Summary:Uterine flushings collected at Day 0, 3, 5 and 7 after estrus

Sample Preparation:

Sampleprep ID:SP000600
Sampleprep Summary:None
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000879 AN000880
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000624
Methods Filename:Metabolomics_LCMSProtocol_plasma.pdf
Chromatography Comments:Ensure that Column is an ACE Excel 2 C18-PFP with dimensions of 100 x 2.1mm, 2.0 μm with a Halo C18-PFP guard attached
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000780
Analysis ID:AN000879
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000781
Analysis ID:AN000880
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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