Summary of Study ST000580

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000425. The data can be accessed directly via it's Project DOI: 10.21228/M84305 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000580
Study TitleDBA2J Phosphatidylethserine Analysis
Study SummaryAH and TM samples were obtained from young-normotensive DBA/2J (3 and 7.5 months) and old-hypertensive DBA/2J mice (8-8.5, 10 and 12 month). Lipids were extracted using modified Bligh and Dyer method and subjected to mass spectrometric identification using appropriate class-specific lipid standards and ratiometric quantification. Corresponding aqueous phase (of extraction) protein concentrations were measured using Bradford method.
Institute
University of Miami
Last NameBhattacharya
First NameSanjoy
AddressMcKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136
EmailSBhattacharya@med.miami.edu
Phone305-482-4103
Submit Date2017-04-03
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailMS(Dir. Inf.)
Release Date2019-05-15
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M84305
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000425
Project DOI:doi: 10.21228/M84305
Project Title:Phosphatidylethanolamine Analysis in Aqueous Humor and Trabecular Meshwork of DBA/2J mice
Project Summary:Compared Phosphatidylethanolamine (PE) profiles within aqueous humor (AH) and trabecular meshwork (TM) of normotensive and hypertensive DBA/2J mice using direct infusion triple quadrupole mass spectrometry.
Institute:University of Miami
Last Name:Bhattacharya
First Name:Sanjoy
Address:McKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136
Email:Sbhattacharya@med.miami.edu
Phone:305-482-4103

Subject:

Subject ID:SU000603
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:DBA/2J
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Tissue Location Sex Phenotype
SA030737DBA2J 10M FAHL3AH Female Hypertensive
SA030738DBA2J 8-8.5M FAH3L8AH Female Hypertensive
SA030739DBA2J 12M FAHL6AH Female Hypertensive
SA030740DBA2J 7.5M FAH2L6AH Female Normotensive
SA030741DBA2J 3M FAH2L7AH Female Normotensive
SA030742DBA2J 10M MAHL4AH Male Hypertensive
SA030743DBA2J 8-8.5M MAH2L5AH Male Hypertensive
SA030744DBA2J 12M MAHL6AH Male Hypertensive
SA030745DBA2J 7.5M MAHL6AH Male Normotensive
SA030746DBA2J 3M MAHL5AH Male Normotensive
SA030747DBA2J 10M FTML2TM Female Hypertensive
SA030748DBA2J 8-8.5M FTM3L6TM Female Hypertensive
SA030749DBA2J 12M FTML6TM Female Hypertensive
SA030750DBA2J 3M FTML3TM Female Normotensive
SA030751DBA2J 7.5M FTM2L5TM Female Normotensive
SA030752DBA2J 8-8.5M MTM2L8TM Male Hypertensive
SA030753DBA2J 10M MTML2TM Male Hypertensive
SA030754DBA2J 12M MTML6TM Male Hypertensive
SA030755DBA2J 7.5M MTML6TM Male Normotensive
SA030756DBA2J 3M MTML5TM Male Normotensive
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000597
Collection Summary:DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to AH procurement. Normotensive and hypertensive AH was obtained from the anterior chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog no. 7803-05 Hamilton). TM samples were obtained through surgical removal after euthanization with carbon dioxide and adhering to animal procedures approved by the IACUC.
Sample Type:Eye tissue

Treatment:

Treatment ID:TR000617
Treatment Summary:No treatment was performed on the mice.

Sample Preparation:

Sampleprep ID:SP000610
Sampleprep Summary:A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method.

Combined analysis:

Analysis ID AN000891
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system TSQ Quantum Access Max
Column None
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva Access Max
Ion Mode NEGATIVE
Units pmol/ ug of protein

Chromatography:

Chromatography ID:CH000634
Instrument Name:TSQ Quantum Access Max
Column Name:None
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS000793
Analysis ID:AN000891
Instrument Name:Thermo Quantiva Access Max
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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