Summary of Study ST000582

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000425. The data can be accessed directly via it's Project DOI: 10.21228/M84305 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000582
Study TitleDBA2J Phospholipid Analysis
Study SummaryAH and TM samples were obtained from young-normotensive DBA/2J (3 and 7.5 months) and old-hypertensive DBA/2J mice (8-8.5, 10 and 12 month). Lipids were extracted using modified Bligh and Dyer method and subjected to mass spectrometric identification using appropriate class-specific lipid standards and ratiometric quantification. Corresponding aqueous phase (of extraction) protein concentrations were measured using Bradford method.
Institute
University of Miami
Last NameBhattacharya
First NameSanjoy
AddressMcKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136
EmailSBhattacharya@med.miami.edu
Phone305-482-4103
Submit Date2017-02-21
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-03-06
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M84305
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000425
Project DOI:doi: 10.21228/M84305
Project Title:Phosphatidylethanolamine Analysis in Aqueous Humor and Trabecular Meshwork of DBA/2J mice
Project Summary:Compared Phosphatidylethanolamine (PE) profiles within aqueous humor (AH) and trabecular meshwork (TM) of normotensive and hypertensive DBA/2J mice using direct infusion triple quadrupole mass spectrometry.
Institute:University of Miami
Last Name:Bhattacharya
First Name:Sanjoy
Address:McKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136
Email:Sbhattacharya@med.miami.edu
Phone:305-482-4103

Subject:

Subject ID:SU000605
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:DBA/2J
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Tissue Location Sex Phenotype
SA030777DBA2J 8-8.5M FAH4L3AH Female Hypertensive
SA030778DBA2J 10M FAHL4AH Female Hypertensive
SA030779DBA2J 12M FAHL1AH Female Hypertensive
SA030780DBA2J 7.5M FAHL4AH Female Normotensive
SA030781DBA2J 3M FAHL1AH Female Normotensive
SA030782DBA2J 8-8.5M MAH4L3AH Male Hypertensive
SA030783DBA2J 10 MAHL3AH Male Hypertensive
SA030784DBA2J 12M MAHL1AH Male Hypertensive
SA030785DBA2J 3M MAHL1AH Male Normotensive
SA030786DBA2J 7.5M MAH2L1AH Male Normotensive
SA030787DBA2J 8-8.5M FTML2TM Female Hypertensive
SA030788DBA2J 10M FTML3TM Female Hypertensive
SA030789DBA2J 12M FTML1TM Female Hypertensive
SA030790DBA2J 7.5M FTML3TM Female Normotensive
SA030791DBA2J 3M FTML4TM Female Normotensive
SA030792DBA2J 10M MTML3TM Male Hypertensive
SA030793DBA2J 8-8.5M MTM3L3TM Male Hypertensive
SA030794DBA2J 12M MTML1TM Male Hypertensive
SA030795DBA2J 7.5M MTM2L1TM Male Normotensive
SA030796DBA2J 3M MTML1TM Male Normotensive
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000599
Collection Summary:DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to AH procurement. Normotensive and hypertensive AH was obtained from the anterior chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog no. 7803-05 Hamilton). TM samples were obtained through surgical removal after euthanization with carbon dioxide and adhering to animal procedures approved by the IACUC.
Sample Type:Eye tissue

Treatment:

Treatment ID:TR000619
Treatment Summary:No treatment was performed on the mice.

Sample Preparation:

Sampleprep ID:SP000612
Sampleprep Summary:A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method.

Combined analysis:

Analysis ID AN000893
Analysis type MS
Chromatography type Reversed phase
Chromatography system TSQ Quantum Access Max
Column None
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva Access Max
Ion Mode POSITIVE
Units pmol/ ug of protein

Chromatography:

Chromatography ID:CH000636
Instrument Name:TSQ Quantum Access Max
Column Name:None
Chromatography Type:Reversed phase

MS:

MS ID:MS000795
Analysis ID:AN000893
Instrument Name:Thermo Quantiva Access Max
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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