Summary of Study ST000583
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000426. The data can be accessed directly via it's Project DOI: 10.21228/M80892 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000583 |
Study Title | The Metabolomics of Oral Biofilms exposed to Arginine and Fluoride |
Study Summary | The study aims to use global metabolomics to investigate: (1) the metabolic profile of supragingival dental plaque from adults with different caries-status and from specific healthy and carious tooth-sites; and (2) the metabolic changes occurring in response to the use of the arginine or fluoride toothpastes for 12 weeks. |
Institute | University of Florida |
Department | SECIM |
Laboratory | Nascimento/Garrett |
Last Name | Nascimento |
First Name | Marcelle |
Address | 1395 Center Drive, Room D9-6, PO Box 100415, Gainesville, FL 32610-0415 |
mnascimento@dental.ufl.edu | |
Phone | 3522735858 |
Submit Date | 2017-01-12 |
Num Groups | 1 |
Total Subjects | 61 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2018-02-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000426 |
Project DOI: | doi: 10.21228/M80892 |
Project Title: | The Metabolomics of Oral Biofilms exposed to Arginine and Fluoride |
Project Type: | Cariology, Oral Microbiology |
Project Summary: | The study aims to use global metabolomics to investigate: (1) the metabolic profile of supragingival dental plaque from adults with different caries-status and from specific healthy and carious tooth-sites; and (2) the metabolic changes occurring in response to the use of the arginine or fluoride toothpastes for 12 weeks. |
Institute: | University of Florida |
Department: | Restorative Dental Sciences |
Laboratory: | Nascimento |
Last Name: | Nascimento |
First Name: | Marcelle |
Address: | 1395 Center Drive, Room D9-6, PO Box 100415, Gainesville, FL 32610-0415 |
Email: | mnascimento@dental.ufl.edu |
Phone: | 3522735858 |
Funding Source: | Colgate Palmolive |
Subject:
Subject ID: | SU000606 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | Contains oral bacteria species |
Age Or Age Range: | 18-65 years |
Gender: | F/M |
Human Race: | Various |
Human Ethnicity: | Various |
Human Lifestyle Factors: | Various |
Human Medications: | None |
Human Prescription Otc: | None |
Human Smoking Status: | None |
Human Alcohol Drug Use: | None |
Human Nutrition: | Various |
Human Inclusion Criteria: | Age 18 to 65 years, caries-free or caries-actie, no systemic disease |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | caries status | time |
---|---|---|---|
SA030797 | G10_6 | caries-active | 12-weeks |
SA030798 | G14_9 | caries-active | 12-weeks |
SA030799 | G14_7 | caries-active | 12-weeks |
SA030800 | G12_6 | caries-active | 12-weeks |
SA030801 | G10_7 | caries-active | 12-weeks |
SA030802 | G14_10 | caries-active | 12-weeks |
SA030803 | G14_6 | caries-active | 12-weeks |
SA030804 | G14_8 | caries-active | 12-weeks |
SA030805 | G16_7 | caries-active | 12-weeks |
SA030806 | G16_8 | caries-active | 12-weeks |
SA030807 | G16_6 | caries-active | 12-weeks |
SA030808 | G13_8 | caries-active | baseline |
SA030809 | G13_9 | caries-active | baseline |
SA030810 | G11_6 | caries-active | baseline |
SA030811 | G13_6 | caries-active | baseline |
SA030812 | G13_7 | caries-active | baseline |
SA030813 | G15_8 | caries-active | baseline |
SA030814 | G15_7 | caries-active | baseline |
SA030815 | G15_6 | caries-active | baseline |
SA030816 | G9_7 | caries-active | baseline |
SA030817 | G9_6 | caries-active | baseline |
SA030818 | G8_7 | caries-free | 12-weeks |
SA030819 | G8_10 | caries-free | 12-weeks |
SA030820 | G8_9 | caries-free | 12-weeks |
SA030821 | G8_8 | caries-free | 12-weeks |
SA030822 | G6_10 | caries-free | 12-weeks |
SA030823 | G8_6 | caries-free | 12-weeks |
SA030824 | G6_6 | caries-free | 12-weeks |
SA030825 | G4_7 | caries-free | 12-weeks |
SA030826 | G4_8 | caries-free | 12-weeks |
SA030827 | G4_6 | caries-free | 12-weeks |
SA030828 | G2_8 | caries-free | 12-weeks |
SA030829 | G2_9 | caries-free | 12-weeks |
SA030830 | G4_9 | caries-free | 12-weeks |
SA030831 | G4_10 | caries-free | 12-weeks |
SA030832 | G6_7 | caries-free | 12-weeks |
SA030833 | G6_8 | caries-free | 12-weeks |
SA030834 | G2_10 | caries-free | 12-weeks |
SA030835 | G2_6 | caries-free | 12-weeks |
SA030836 | G2_7 | caries-free | 12-weeks |
SA030837 | G6_9 | caries-free | 12-weeks |
SA030838 | G7_6 | caries-free | baseline |
SA030839 | G3_7 | caries-free | baseline |
SA030840 | G3_8 | caries-free | baseline |
SA030841 | G3_9 | caries-free | baseline |
SA030842 | G3_6 | caries-free | baseline |
SA030843 | G1_10 | caries-free | baseline |
SA030844 | G1_7 | caries-free | baseline |
SA030845 | G1_8 | caries-free | baseline |
SA030846 | G1_9 | caries-free | baseline |
SA030847 | G3_10 | caries-free | baseline |
SA030848 | G5_6 | caries-free | baseline |
SA030849 | G7_7 | caries-free | baseline |
SA030850 | G7_8 | caries-free | baseline |
SA030851 | G7_9 | caries-free | baseline |
SA030852 | G1_6 | caries-free | baseline |
SA030853 | G5_10 | caries-free | baseline |
SA030854 | G5_7 | caries-free | baseline |
SA030855 | G5_8 | caries-free | baseline |
SA030856 | G5_9 | caries-free | baseline |
SA030857 | G7_10 | caries-free | baseline |
Showing results 1 to 61 of 61 |
Collection:
Collection ID: | CO000600 |
Collection Summary: | Supragingival plaque samples were collected from specific tooth sites using sterile dental curetes. |
Sample Type: | Plaque samples |
Collection Method: | Scrapping of tooth surfaces |
Collection Location: | Multiple teeth |
Collection Frequency: | Once |
Collection Duration: | 5 min |
Volumeoramount Collected: | 250ul |
Storage Conditions: | freezer at -80 |
Collection Vials: | eppendorfs containing phosphate buffer pH 7.0 |
Storage Vials: | eppendorfs containing phosphate buffer pH 7.0 |
Collection Tube Temp: | 4 |
Treatment:
Treatment ID: | TR000620 |
Treatment Summary: | Subjects made use for 12 weeks of either a fluoride-free 1.5% arginine toothpaste or a fluoride containing toothpaste (1100 ppm F as NaF). |
Sample Preparation:
Sampleprep ID: | SP000613 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
Analysis ID | AN000894 | AN000895 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | unspecified | unspecified |
Chromatography:
Chromatography ID: | CH000637 |
Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000796 |
Analysis ID: | AN000894 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000797 |
Analysis ID: | AN000895 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |