Summary of Study ST000600
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000437. The data can be accessed directly via it's Project DOI: 10.21228/M8K31K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000600 |
| Study Title | NEFA Profile Response to Triphenyl Phosphate Exposure |
| Study Summary | This study aims to identify changes in non-esterified fatty acid (NEFAs) in the plasma with triphenyl phosphate (TPP) exposure. UC Davis type 2 diabetes mellitus (UCD-T2DM) rats were treated with TPP or not treated. Each group was analyzed for non-esterified fatty acid (NEFA) changes to investigate alterations in NEFAs due to TPP exposure. Targeted analysis of NEFA in rat plasma samples was performed by the Newman lab. |
| Institute | University of California, Davis |
| Department | USDA Western Human Nutrition Research Center |
| Last Name | Newman |
| First Name | John |
| Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
| john.newman@ars.usda.gov | |
| Phone | +1-530-752-1009 |
| Submit Date | 2017-04-27 |
| Study Comments | The samples included a high degree of hemolysis exhibited in the plasma. One sample was lost during processing (Group E- Subject 78). Two samples were outliers for multiple analytes and were not included in the final data (E-117 & T-28). Of the samples reported in this data set, there were no missing values. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2017-07-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000437 |
| Project DOI: | doi: 10.21228/M8K31K |
| Project Title: | NEFA Profile Response to Triphenyl Phosphate Exposure |
| Project Summary: | This study aims to identify changes in non-esterified fatty acid (NEFAs) in the plasma with triphenyl phosphate (TPP) exposure. UC Davis type 2 diabetes mellitus (UCD-T2DM) rats were treated with TPP or not treated. Each group was analyzed for non-esterified fatty acid (NEFA) changes to investigate alterations in NEFAs due to TPP exposure. Targeted analysis of NEFA in rat plasma samples was performed by the Newman lab. |
| Institute: | University of California, Davis |
| Department: | Department of Environmental Toxicology |
| Last Name: | La Merrill |
| First Name: | Michele |
| Address: | 1 Shields Ave., Davis, CA 95616 |
| Email: | mlamerrill@ucdavis.edu |
| Phone: | (530) 754-7254 |
Subject:
| Subject ID: | SU000623 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA033113 | LM-24 | E (Vehicle control) |
| SA033114 | LM-22 | E (Vehicle control) |
| SA033115 | LM-14 | E (Vehicle control) |
| SA033116 | LM-16 | E (Vehicle control) |
| SA033117 | LM-15 | E (Vehicle control) |
| SA033118 | LM-01 | E (Vehicle control) |
| SA033119 | LM-10 | E (Vehicle control) |
| SA033120 | LM-31 | E (Vehicle control) |
| SA033121 | LM-13 | E (Vehicle control) |
| SA033122 | LM-12 | E (Vehicle control) |
| SA033123 | LM-09 | E (Vehicle control) |
| SA033124 | LM-06 | E (Vehicle control) |
| SA033125 | LM-08 | E (Vehicle control) |
| SA033126 | LM-32 | E (Vehicle control) |
| SA033127 | LM-19 | T (Treated with TPhP) |
| SA033128 | LM-03 | T (Treated with TPhP) |
| SA033129 | LM-25 | T (Treated with TPhP) |
| SA033130 | LM-07 | T (Treated with TPhP) |
| SA033131 | LM-21 | T (Treated with TPhP) |
| SA033132 | LM-29 | T (Treated with TPhP) |
| SA033133 | LM-11 | T (Treated with TPhP) |
| SA033134 | LM-20 | T (Treated with TPhP) |
| SA033135 | LM-27 | T (Treated with TPhP) |
| SA033136 | LM-05 | T (Treated with TPhP) |
| SA033137 | LM-02 | T (Treated with TPhP) |
| SA033138 | LM-28 | T (Treated with TPhP) |
| SA033139 | LM-17 | T (Treated with TPhP) |
| SA033140 | LM-26 | T (Treated with TPhP) |
| SA033141 | LM-30 | T (Treated with TPhP) |
| Showing results 1 to 29 of 29 |
Collection:
| Collection ID: | CO000617 |
| Collection Summary: | Prior to sacrifice, rats were fasted between 8 and 12 h,their body weights recorded, and blood was collected from the tail as above prior to euthanasia. Rats were anesthetized with sodium pentobarbital and euthanized with a 1 mL/kg intracardiac injection of saturated potassium chloride. Once cardiac movement had stopped for 30 s the rat was decapitated and the hypothalamus, liver, pancreas, heart, mesenteric adipose tissue, quadriceps, kidney, gonadal adipose tissue, inguinal adipose tissue, and brown adipose tissue were collected. All tissues were removed in the order listed above, wet weighed, and snap frozen in liquid nitrogen |
| Collection Protocol Filename: | Green_et_al_2017-TPP_Exposure_Accelerat_T2DM_Rats.pdf |
| Sample Type: | Blood |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000637 |
| Treatment Summary: | Adult non-pregnant female UCD-T2DM rats (n = 16; 3 months old) were paired with males (n = 10; 3–4 months old) for a 24 h period at which point males were removed. This was defined as gestational day zero (G0) if a sperm plug was observed or if the female rats gained at least 30 g of body weight over the next 7 days. The day of birth was designated postnatal day zero (P0). Pregnant dams were randomly assigned to an exposure group (n = 8 per group), and received daily oral TPhP or ethanol vehicle exposure from G8 through weaning (P21) as described in Section 2.2 below. Gestational length and litter size were recorded on P0 and the sex of pups was determined and recorded on P4. Body weights of all pups in each litter were obtained periodically from P4–21. On P4 the litters were culled to 8 pups ensuring up to 4males and 2 females in each litter by random selection (Fig. 1A & B). This was done to ensure consistent exposure of pups between litters [13,23]. The time ittakes to develop T2DM is accelerated among UCD-T2DM rats with higher body weights on P21. Hence at weaning the largest pups were housed in same sex littermate groups of two females and up to four males as available (Fig. 1A & B). Urine was collected from the dams using an adapted plastic wrap method outlined by Kurien [24], 60 mins after final dose. Dams were placed in clean cages without bedding for at least 20 min then using a pipette up to 500 L of urine was collected in ethanol rinsed glass vials and placed on ice. At weaning all dams and remaining weanlings were sacrificed (90–330 min post-exposure) by CO2 asphyxiation and rapid decapitation. Two male rats weighing between 350–400 g on P61, from the TPhP group and the vehicle group were weight-matched across treatments for the diabetes study to eliminate confounding effects of body mass on the association between TPhP and T2DM onset (Fig. 1B). This weight range was selected because male UCD-T2DM rats that are between 350 and 400 g at 8 weeks of age develop T2DM at approximately 23 weeks of age [18]. Weight-matched rats were followed until 26 weeks or until they developed T2DM, which was defined as two consecutive weekly non-fasting glucose measurements of ≥200 mg/dL [18] in accordance with the American Diabetes Association (ADA) guideline of diagnosing diabetes with a random plasma glucose of 200 mg/dL or higher [19]. The remaining rats were not weight-matched and followed for the 3.5 month obesity study (Fig. 1A). |
| Treatment Protocol Filename: | Green_et_al_2017-TPP_Exposure_Accelerat_T2DM_Rats.pdf |
Sample Preparation:
| Sampleprep ID: | SP000630 |
| Sampleprep Summary: | Plasma non-esterified fatty acids (NEFAs) were isolated as previously described by Smedes (1). and Gladine et al. (2). Specifically, plasma aliquots (100 mL) were enriched with 5 mL 0.2 mg/ml butylated hydroxytoluene/EDTA in 1:1 methanol:- water, and a suite of extraction surrogates, which included deuterated-tri-palmitoyl glycerol (d31-16:0-TG; CDN Isotopes, Pointe-Claire, Quebec, Canada), deuterated distearoylphosphotidylcholine (d35-18:0-PC; Avanti Polar Lipids, Alabaster, Alabama), dodeca-(9E)-enoyl cholesterylesters (22:1n9-CE; NuChek Prep, Elysian MN) and dodecatrienoic acid (22:3n3; NuChek Prep). Lipids were then extracted with 10:8:11 cylcohexane: 2- propanol:ammonium acetate. Briefly, enriched samples were mixed with cyclopropane/2-propanol, phases were split with ammonium acetate, the organic phase was isolated and the aqueous phase was re-extracted with cyclohexane. The combined organic total lipid extract was reduced to dryness and reconstituted in 200 µL of 1:1 methanol/toluene and the total lipid extract was used to quantify plasma fatty acids as methyl esters by gas chromatography-mass spectrometry (GC-MS). It was derivitized by adding 45 μL 2M (trimethylsilyl) diazomethane in hexanes and spiked with 15:1n5 free acid to track methylation efficiency. Next, it was brought to a final volume of 200 mL with 90:10 methanol/toluene (v/v) and left at room temperature for 30 min, before being brought to dryness. The remaining fatty acid methyl esters (FAMEs) were re-constituted in 300 mL Hexane plus 10 uL of 44 mM tricosanoate methyl ester (23:0; NuChek Prep), vortexed, and 100 uL was transferred to a GC-MS Vial for analysis. |
| Sampleprep Protocol Filename: | NEFA_Plasma_Newman_Data_Report.docx |
Combined analysis:
| Analysis ID | AN000919 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB-225 (30m x 0.25mm x 0.5um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent |
| Ion Mode | POSITIVE |
| Units | Concentration (µM) |
Chromatography:
| Chromatography ID: | CH000655 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB-225 (30m x 0.25mm x 0.5um) |
| Sample Injection: | 1 µl |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000818 |
| Analysis ID: | AN000919 |
| Instrument Name: | Agilent |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
