Summary of Study ST000618

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000428. The data can be accessed directly via it's Project DOI: 10.21228/M8QW3H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000618
Study TitleMetabolomic Profiling of Follicular Fluid from Patients with Polycystic Ovary Syndrome and Hyper Response to In Vitro Fertilization Treatment
Study TypeMS quallitative analysis
Study SummaryThe present study consisted in a metabolomic approach in follicular fluid samples from patients with polycystic ovary syndrome and hyper response to in vitro fertilization treatment.
Institute
Sao Paulo Federal University
DepartmentDepartment of Surgery, Division of Urology, Human Reproduction Section
LaboratoryCenter of research in Urology
Last NameCordeiro
First NameFernanda
AddressRua Embau, 231, Vila Clementino
Emailfernandabertuccez85@gmail.com
Phone11996667402
Submit Date2017-05-30
Num Groups3
Total Subjects81
Num Females81
Analysis Type DetailLC-MS
Release Date2017-11-20
Release Version1
Fernanda Cordeiro Fernanda Cordeiro
https://dx.doi.org/10.21228/M8QW3H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000428
Project DOI:doi: 10.21228/M8QW3H
Project Title:Follicular fluid Metabolomics
Project Type:MS qualitative analysis
Project Summary:Metabolomics profiling of follicular fluid samples from patients undergoing in vitro fertilization treatment
Institute:Sao Paulo Federal University
Department:Department of Surgery, Division of Urology, Human Reproduction Section
Laboratory:Center Of Research In Urology
Last Name:Cordeiro
First Name:Fernanda
Address:Rua Embau, 231, Vila Clementino
Email:fernandabertuccez85@gmail.com
Phone:11996667402

Subject:

Subject ID:SU000641
Subject Type:Human Follicular Fluid
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:female
Human Exclusion Criteria:cancer, endometriosis, premature ovarian failure
Species Group:Mammals

Factors:

Subject type: Human Follicular Fluid; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Condition
SA034234C15_2Control
SA034235C15_1Control
SA034236QC_Control_6Control
SA034237C16_1Control
SA034238C16_2Control
SA034239C14_2Control
SA034240C14_1Control
SA034241C12_1Control
SA034242C12_2Control
SA034243C13_1Control
SA034244C13_2Control
SA034245C17_1Control
SA034246C17_2Control
SA034247C21_1Control
SA034248C21_2Control
SA034249C22_1Control
SA034250QC_Control_1Control
SA034251C20_2Control
SA034252C20_1Control
SA034253C18_1Control
SA034254C18_2Control
SA034255C19_1Control
SA034256C19_2Control
SA034257C11_2Control
SA034258C22_2Control
SA034259C03_2Control
SA034260C03_1Control
SA034261C04_1Control
SA034262C04_2Control
SA034263C05_1Control
SA034264C02_2Control
SA034265C02_1Control
SA034266C11_1Control
SA034267QC_Control_2Control
SA034268C01_1Control
SA034269C01_2Control
SA034270C05_2Control
SA034271QC_Control_3Control
SA034272C09_2Control
SA034273C09_1Control
SA034274C10_1Control
SA034275QC_Control_4Control
SA034276QC_Control_5Control
SA034277C08_2Control
SA034278C10_2Control
SA034279C08_1Control
SA034280C06_2Control
SA034281C06_1Control
SA034282C07_2Control
SA034283C07_1Control
SA034284HR30_2Hyper Responder
SA034285HR30_1Hyper Responder
SA034286HR29_1Hyper Responder
SA034287HR29_2Hyper Responder
SA034288HR31_1Hyper Responder
SA034289HR33_1Hyper Responder
SA034290HR33_2Hyper Responder
SA034291HR32_2Hyper Responder
SA034292HR32_1Hyper Responder
SA034293HR28_2Hyper Responder
SA034294HR31_2Hyper Responder
SA034295QC_HR_9Hyper Responder
SA034296HR27_2Hyper Responder
SA034297HR24_2Hyper Responder
SA034298HR24_1Hyper Responder
SA034299HR34_1Hyper Responder
SA034300HR23_2Hyper Responder
SA034301HR25_1Hyper Responder
SA034302HR25_2Hyper Responder
SA034303HR27_1Hyper Responder
SA034304HR26_2Hyper Responder
SA034305HR26_1Hyper Responder
SA034306QC_HR_8Hyper Responder
SA034307HR28_1Hyper Responder
SA034308HR40_1Hyper Responder
SA034309HR42_1Hyper Responder
SA034310HR42_2Hyper Responder
SA034311HR41_2Hyper Responder
SA034312HR41_1Hyper Responder
SA034313QC_HR_11Hyper Responder
SA034314HR43_1Hyper Responder
SA034315HR43_2Hyper Responder
SA034316HR23_1Hyper Responder
SA034317QC_HR_12Hyper Responder
SA034318HR44_2Hyper Responder
SA034319HR44_1Hyper Responder
SA034320HR40_2Hyper Responder
SA034321HR39_2Hyper Responder
SA034322HR36_1Hyper Responder
SA034323QC_HR_10Hyper Responder
SA034324HR35_2Hyper Responder
SA034325HR35_1Hyper Responder
SA034326HR36_2Hyper Responder
SA034327HR37_1Hyper Responder
SA034328HR39_1Hyper Responder
SA034329HR38_2Hyper Responder
SA034330HR38_1Hyper Responder
SA034331HR37_2Hyper Responder
SA034332HR34_2Hyper Responder
SA034333HR19_2Hyper Responder
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Collection:

Collection ID:CO000635
Collection Summary:The patients were initially undergoing to controlled ovarian stimulation, from which follicular fluid samples were collected.
Sample Type:Follicular fluid

Treatment:

Treatment ID:TR000655
Treatment Summary:Follicular fluid samples were centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction.

Sample Preparation:

Sampleprep ID:SP000648
Sampleprep Summary:50 µL of follicular fluid samples were submitted to Bligh & Dyer protocol.
Extraction Method:BLIGH & DYER
Sample Resuspension:5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v)

Chromatography:

Chromatography ID:CH000675
Chromatography Summary:Liquid Chromatography was performed by using a chromatographer Acquity with a reverse phase column (Acquity UPCL HSS 1,8 µm, 2.1x100 mm - Waters Corporation, MA, USA), at 35 °C. The solvents for the elution were: A- H2O with formic acid 0.1% and B- ACN with formic acid 0.1%. The initial gradient was composed of 80% of A and 20% of B, during 1 minute. Next, the gradient was linearly changed for 50% of A and 50% of B, during the next minute. In the sequence, the gradient was changed for 35% of A and 65% of B during 2 minutes. The gradient was then inverted, considering 20% of A and 80% of B, during 3 minutes. For the next 10 minutes, the gradient was 3% of A and 97% of B. The initial condition was reestablished for the column washing during 1 minute. The total run time was 17 minutes and the solvents flux was 0.5 mL/min and the total amount of sample used per run was 5 µL.
Instrument Name:Ultima Quadrupole-Time of Flight - Waters Corporation
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:35 °C
Flow Rate:0.5 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN000948
Analysis Type:MS
Instrument Name:Ultima Quadrupole-Time of Flight (Ultima Q-TOF - Waters Corporation, MA, USA)
Chromatography ID:CH000675
Num Factors:3
Has Mz:1
Results File:ST000618_AN000948_Results.txt
Units:mass intensity
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