Summary of Study ST000640

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000458. The data can be accessed directly via it's Project DOI: 10.21228/M8VG74 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000640
Study Title	Targeted NEFA in American Indian Adolescents (part I)
Study Summary	The goal of this study is to determine the effect of physical activity on the blood concentrations of several compounds that are proposed to be markers of diabetic risk. We will compare results from normal weight and obese American Indian adolescents, with high or low habitual physical activity. We will also compare the same blood markers in the obese group before and after 4 months of exercise to measure the benefit of the program.
Institute	Mayo Clinic
Last Name	Short
First Name	Kevin
Address	University of Oklahoma Health Sciences Center 1200 Children’s Ave, Suite 4500 Oklahoma City, OK 73104
Email	kevin-short@ouhsc.edu
Phone	405-271-8001
Submit Date	2017-06-23
Analysis Type Detail	LC-MS
Release Date	2021-01-19
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000458
Project DOI:	doi: 10.21228/M8VG74
Project Title:	Biomarkers of diabetes risk in children: role of obesity and exercise
Project Summary:	This proposal addresses a gap in knowledge about biomarkers for insulin resistance and diabetes. It remains unclear why some populations develop diabetes at higher rates than others and whether prevention and treatment strategies should also differ. The main goal of the current study is to determine the impact of body composition and physical activity on biomarkers for insulin resistance in American Indian adolescents. We propose to use a targeted metabolomic approach to measure amino compounds and fatty acids in plasma. We will address these goals by exploring two Specific Aims: 1. To determine the effects of obesity and physical activity on circulating biomarkers for diabetes and metabolic disease risk in American Indian youth. Plasma samples used for measurements of amino compounds and fatty acids will be acquired from boys and girls 11-21 years old classified as, a) obese, low habitual physical activity and low fitness; b) normal weight, low habitual physical activity and low fitness; or, c) normal weight, high habitual physical activity and high fitness. 2. To determine the impact of exercise training on circulating biomarkers for diabetes and metabolic disease risk in obese American Indian youth. The same obese group tested in the cross-sectional comparison in Aim 1 will complete 16 weeks of aerobic exercise training. Plasma samples acquired before and after training will be used to measure amino compounds and fatty acids. The results of this project will provide novel information about the metabolic changes associated with insulin resistance, obesity, and physical activity in young people who are at risk for, but have not yet developed cardiometabolic disease. The study design takes advantage of, and supports the scientific partnership among investigators at the University of Oklahoma Health Sciences Center, Choctaw Nation of Oklahoma, and Mayo Clinic Rochester. This will set the stage for future lifestyle intervention studies to further explore the concepts outlined in this proposal.
Institute:	Mayo Clinic
Last Name:	Short
First Name:	Kevin
Address:	University of Oklahoma Health Sciences Center 1200 Children’s Ave, Suite 4500 Oklahoma City, OK 73104
Email:	kevin-short@ouhsc.edu
Phone:	405-271-8001

Subject:

Subject ID:	SU000663
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Time	Study Group
SA036015	133	baseline	normal weight reference group
SA036018	125	baseline	normal weight reference group
SA036019	123	baseline	normal weight reference group
SA036040	129	baseline	normal weight reference group
SA036041	127	baseline	normal weight reference group
SA036045	131	baseline	normal weight reference group
SA036141	119	baseline	normal weight reference group
SA036142	121	baseline	normal weight reference group
SA036011	97	baseline	Overweight/Obese 48 week exercise program
SA036012	95	baseline	Overweight/Obese 48 week exercise program
SA036013	117	baseline	Overweight/Obese 48 week exercise program
SA036016	87	baseline	Overweight/Obese 48 week exercise program
SA036017	15	baseline	Overweight/Obese 48 week exercise program
SA036020	11	baseline	Overweight/Obese 48 week exercise program
SA036021	17	baseline	Overweight/Obese 48 week exercise program
SA036022	73	baseline	Overweight/Obese 48 week exercise program
SA036023	21	baseline	Overweight/Obese 48 week exercise program
SA036025	31	baseline	Overweight/Obese 48 week exercise program
SA036026	13	baseline	Overweight/Obese 48 week exercise program
SA036033	89	baseline	Overweight/Obese 48 week exercise program
SA036036	93	baseline	Overweight/Obese 48 week exercise program
SA036038	33	baseline	Overweight/Obese 48 week exercise program
SA036042	77	baseline	Overweight/Obese 48 week exercise program
SA036043	75	baseline	Overweight/Obese 48 week exercise program
SA036046	23	baseline	Overweight/Obese 48 week exercise program
SA036047	115	baseline	Overweight/Obese 48 week exercise program
SA036048	113	baseline	Overweight/Obese 48 week exercise program
SA036050	91	baseline	Overweight/Obese 48 week exercise program
SA036052	111	baseline	Overweight/Obese 48 week exercise program
SA036053	99	baseline	Overweight/Obese 48 week exercise program
SA036057	35	baseline	Overweight/Obese 48 week exercise program
SA036058	37	baseline	Overweight/Obese 48 week exercise program
SA036061	81	baseline	Overweight/Obese 48 week exercise program
SA036062	79	baseline	Overweight/Obese 48 week exercise program
SA036065	41	baseline	Overweight/Obese 48 week exercise program
SA036066	39	baseline	Overweight/Obese 48 week exercise program
SA036067	25	baseline	Overweight/Obese 48 week exercise program
SA036068	49	baseline	Overweight/Obese 48 week exercise program
SA036069	51	baseline	Overweight/Obese 48 week exercise program
SA036072	47	baseline	Overweight/Obese 48 week exercise program
SA036073	43	baseline	Overweight/Obese 48 week exercise program
SA036074	45	baseline	Overweight/Obese 48 week exercise program
SA036081	27	baseline	Overweight/Obese 48 week exercise program
SA036083	55	baseline	Overweight/Obese 48 week exercise program
SA036088	53	baseline	Overweight/Obese 48 week exercise program
SA036094	57	baseline	Overweight/Obese 48 week exercise program
SA036100	65	baseline	Overweight/Obese 48 week exercise program
SA036101	63	baseline	Overweight/Obese 48 week exercise program
SA036103	101	baseline	Overweight/Obese 48 week exercise program
SA036110	59	baseline	Overweight/Obese 48 week exercise program
SA036111	61	baseline	Overweight/Obese 48 week exercise program
SA036114	29	baseline	Overweight/Obese 48 week exercise program
SA036121	1	baseline	Overweight/Obese 48 week exercise program
SA036123	3	baseline	Overweight/Obese 48 week exercise program
SA036129	7	baseline	Overweight/Obese 48 week exercise program
SA036130	67	baseline	Overweight/Obese 48 week exercise program
SA036132	107	baseline	Overweight/Obese 48 week exercise program
SA036133	109	baseline	Overweight/Obese 48 week exercise program
SA036137	69	baseline	Overweight/Obese 48 week exercise program
SA036138	83	baseline	Overweight/Obese 48 week exercise program
SA036140	103	baseline	Overweight/Obese 48 week exercise program
SA036147	105	baseline	Overweight/Obese 48 week exercise program
SA036151	71	baseline	Overweight/Obese 48 week exercise program
SA036152	85	baseline	Overweight/Obese 48 week exercise program
SA036154	9	baseline	Overweight/Obese 48 week exercise program
SA036155	19	baseline	Overweight/Obese 48 week exercise program
SA036014	153	baseline	Overweight/Obese 5 week exercise program
SA036051	151	baseline	Overweight/Obese 5 week exercise program
SA036056	155	baseline	Overweight/Obese 5 week exercise program
SA036082	157	baseline	Overweight/Obese 5 week exercise program
SA036084	137	baseline	Overweight/Obese 5 week exercise program
SA036085	163	baseline	Overweight/Obese 5 week exercise program
SA036086	159	baseline	Overweight/Obese 5 week exercise program
SA036087	161	baseline	Overweight/Obese 5 week exercise program
SA036093	139	baseline	Overweight/Obese 5 week exercise program
SA036095	141	baseline	Overweight/Obese 5 week exercise program
SA036096	167	baseline	Overweight/Obese 5 week exercise program
SA036097	145	baseline	Overweight/Obese 5 week exercise program
SA036098	143	baseline	Overweight/Obese 5 week exercise program
SA036104	135	baseline	Overweight/Obese 5 week exercise program
SA036106	177	baseline	Overweight/Obese 5 week exercise program
SA036107	165	baseline	Overweight/Obese 5 week exercise program
SA036113	175	baseline	Overweight/Obese 5 week exercise program
SA036115	171	baseline	Overweight/Obese 5 week exercise program
SA036116	173	baseline	Overweight/Obese 5 week exercise program
SA036122	169	baseline	Overweight/Obese 5 week exercise program
SA036125	147	baseline	Overweight/Obese 5 week exercise program
SA036139	149	baseline	Overweight/Obese 5 week exercise program
SA036007	108	post-program	Overweight/Obese 48 week exercise program
SA036008	86	post-program	Overweight/Obese 48 week exercise program
SA036009	12	post-program	Overweight/Obese 48 week exercise program
SA036010	10	post-program	Overweight/Obese 48 week exercise program
SA036024	58	post-program	Overweight/Obese 48 week exercise program
SA036027	102	post-program	Overweight/Obese 48 week exercise program
SA036028	66	post-program	Overweight/Obese 48 week exercise program
SA036029	64	post-program	Overweight/Obese 48 week exercise program
SA036030	4	post-program	Overweight/Obese 48 week exercise program
SA036031	30	post-program	Overweight/Obese 48 week exercise program
SA036032	2	post-program	Overweight/Obese 48 week exercise program
SA036034	60	post-program	Overweight/Obese 48 week exercise program

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Collection:

Collection ID:	CO000657
Collection Summary:	Male and female American Indians 11-21 years old are being recruited from the Choctaw Nation Health Service Area of southeast Oklahoma. We use a collaborative approach as recently described by our American Indian Programs Coordinator (12). The primary characteristics of the three study groups are shown in Table 1. Group 1 is obese (Body mass index, BMI, ≥95th percentile for age- and sex-specific norms based on growth charts from the Centers for Disease Control), with pubertal maturation level ≥Tanner Stage 2, family history of T2D, and recent history of low physical activity. Low physical activity, defined as attaining <30 minutes of structured moderate-to-vigorous intensity exercise on 3 days/week over the preceding 3 months, is confirmed through questionnaires and use of an accelerometer. Groups 2 and 3 are both classified as normal weight (BMI greater than 20th and less than 85th percentile) with maturation level ≥Tanner Stage 2. Group 2 will have low physical activity (same as Group 1), while Group 3 will have high physical activity, defined as >30 minutes of structured moderate-to-vigorous intensity exercise on >3 days/week in the preceding 3 months. Additional eligibility criteria are: lack of diabetes or other potentially confounding metabolic disorders, able to safely exercise, willingness to complete the testing and participation schedule, and not on medications known to impact the stated outcomes. A medical history and physical exam is performed to assure suitability for the study during the initial screening.
Sample Type:	Blood

Treatment:

Treatment ID:	TR000677
Treatment Summary:	Only Group 1 participants continue on to the exercise training program, which lasts 48 weeks. Follow-up measurements of the clinical and functional tests are performed at 16, 32, and 48 weeks; values at 16 weeks will be used for the current proposal to assure adequate sample size. Exercise sessions are performed at the Choctaw Wellness Centers, where fitness professionals provide instruction and supervision. Participants receive incentives for completing 20-60 minutes of moderate-to-vigorous physical activity (MVPA) on 3 days per week. MVPA is defined as exercise that elicits a heart rate ≥ 70% of peak heart rate. Several types of exercise can be selected, including walking, running, cycling, aerobic dance or other activities. To promote healthy behavior within the community and foster social support, all participants are invited to exercise with family members or friends if they choose. Exercise intensity is monitored by recording heart rate with a chest strap transmitter that outputs to a computer for quantification and data storage. Training sessions are also recorded in an exercise log by each participant and confirmed by fitness center staff.

Treatment ID:

TR000677

Treatment Summary:

Only Group 1 participants continue on to the exercise training program, which lasts 48 weeks. Follow-up measurements of the clinical and functional tests are performed at 16, 32, and 48 weeks; values at 16 weeks will be used for the current proposal to assure adequate sample size. Exercise sessions are performed at the Choctaw Wellness Centers, where fitness professionals provide instruction and supervision. Participants receive incentives for completing 20-60 minutes of moderate-to-vigorous physical activity (MVPA) on 3 days per week. MVPA is defined as exercise that elicits a heart rate ≥ 70% of peak heart rate. Several types of exercise can be selected, including walking, running, cycling, aerobic dance or other activities. To promote healthy behavior within the community and foster social support, all participants are invited to exercise with family members or friends if they choose. Exercise intensity is monitored by recording heart rate with a chest strap transmitter that outputs to a computer for quantification and data storage. Training sessions are also recorded in an exercise log by each participant and confirmed by fitness center staff.

Sample Preparation:

Sampleprep ID:	SP000670
Sampleprep Summary:	Part 1: Longer-term exercise training study: Samples #1-133. Overweight/Obese adolescents (IDs 434-001 through 434-132) enrolled into a 48-week exercise program (3 days per week). Samples submitted are from the pre-training (Baseline) and the end of the first 16 weeks (Post-training) times. Compliance with the exercise program varies widely among participants. An additional set of normal weight reference group adolescents (IDs 434-301 through 434-308) are included. Note that 15 OW/OB participants in the exercise group and all 8 NW reference group participants have only a single baseline sample. All samples were collected following overnight fast into EDTA (lavender top) glass tubes for preparation of plasma, kept on ice 20 minutes, spun, aliquoted and then kept frozen. None have been previously used. Part 2: Short-term exercise training study: Samples with ID 11xxx. Overweight/Obese adolescents completed a short-term exercise program, consisting of 10 sessions over 5 weeks. Samples were collected following overnight fast, before (Baseline) and after (Post-training) the training program. These samples are serum since plasma was used for other analyses. All tubes are marked with a unique ID number (shown in list below) in black marker. Additional sample details on the labels are listed in the accompanying Excel spreadsheet.

Sampleprep ID:

SP000670

Sampleprep Summary:

Part 1: Longer-term exercise training study: Samples #1-133. Overweight/Obese adolescents (IDs 434-001 through 434-132) enrolled into a 48-week exercise program (3 days per week). Samples submitted are from the pre-training (Baseline) and the end of the first 16 weeks (Post-training) times. Compliance with the exercise program varies widely among participants. An additional set of normal weight reference group adolescents (IDs 434-301 through 434-308) are included. Note that 15 OW/OB participants in the exercise group and all 8 NW reference group participants have only a single baseline sample. All samples were collected following overnight fast into EDTA (lavender top) glass tubes for preparation of plasma, kept on ice 20 minutes, spun, aliquoted and then kept frozen. None have been previously used. Part 2: Short-term exercise training study: Samples with ID 11xxx. Overweight/Obese adolescents completed a short-term exercise program, consisting of 10 sessions over 5 weeks. Samples were collected following overnight fast, before (Baseline) and after (Post-training) the training program. These samples are serum since plasma was used for other analyses. All tubes are marked with a unique ID number (shown in list below) in black marker. Additional sample details on the labels are listed in the accompanying Excel spreadsheet.

Combined analysis:

Analysis ID	AN000972
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Agilent 1290 Infinity
Column	Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type	ESI
MS instrument type	Triple quadrupole
MS instrument name	Agilent 6460 QQQ
Ion Mode	NEGATIVE
Units	uM

Chromatography:

Chromatography ID:	CH000697
Instrument Name:	Agilent 1290 Infinity
Column Name:	Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS000867
Analysis ID:	AN000972
Instrument Name:	Agilent 6460 QQQ
Instrument Type:	Triple quadrupole
MS Type:	ESI
Ion Mode:	NEGATIVE