Summary of Study ST000660

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000461. The data can be accessed directly via it's Project DOI: 10.21228/M8G609 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000660
Study TitleOmega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice (part I)
Study SummaryIn this study we have compared the metabolic effects of conventional soybean oil to those of genetically modified Plenish soybean oil, that is low in linoleic acid and high in oleic acid. This work builds on our previous study showing that soybean oil, rich in polyunsaturated fats, is more obesogenic and diabetogenic than coconut oil, rich in saturated fats (PMID: 26200659). Here, in order to elucidate the mechanisms responsible for soybean oil induced obesity, we have performed the first ever metabolomics (in plasma and liver) and proteomics on the livers of mice fed the two soybean oil diets (plus those fed a high coconut oil and Viv chow diet). Our results show that the new high oleic soybean oil induces less obesity and adiposity than conventional soybean oil, but can cause hepatomegaly and liver dysfunction. Metabolomic analysis reveals that the hepatic and plasma metabolic profiles differ considerably between the two soybean oils. Hepatic C18 oxylipin metabolites of omega-6 (ω6) and omega-3 (ω3) fatty acids (linoleic and α-linolenic acid, respectively) in the cytochrome P450/soluble epoxide hydrolase pathway were found to correlate positively with obesity.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2017-06-22
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2017-10-03
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M8G609
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000461
Project DOI:doi: 10.21228/M8G609
Project Title:Omega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice
Project Summary:In this study we have compared the metabolic effects of conventional soybean oil to those of genetically modified Plenish soybean oil, that is low in linoleic acid and high in oleic acid. This work builds on our previous study showing that soybean oil, rich in polyunsaturated fats, is more obesogenic and diabetogenic than coconut oil, rich in saturated fats (PMID: 26200659). Here, in order to elucidate the mechanisms responsible for soybean oil induced obesity, we have performed the first ever metabolomics (in plasma and liver) and proteomics on the livers of mice fed the two soybean oil diets (plus those fed a high coconut oil and Viv chow diet). Our results show that the new high oleic soybean oil induces less obesity and adiposity than conventional soybean oil, but can cause hepatomegaly and liver dysfunction. Metabolomic analysis reveals that the hepatic and plasma metabolic profiles differ considerably between the two soybean oils. Hepatic C18 oxylipin metabolites of omega-6 (ω6) and omega-3 (ω3) fatty acids (linoleic and α-linolenic acid, respectively) in the cytochrome P450/soluble epoxide hydrolase pathway were found to correlate positively with obesity.
Institute:University of California, Riverside
Department:Cell Biology and Neuroscience
Last Name:Sladek
First Name:Frances
Address:2115 Biological Sciences Building,University of California, Riverside, CA 92521-0314
Email:frances.sladek@ucr.edu
Phone:951-827-2264

Subject:

Subject ID:SU000967
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57/BL6N
Gender:Male
Animal Housing:SPF facility
Animal Light Cycle:12:12 h light-dark cycle
Animal Feed:Ad libitum
Animal Water:Ad libitum

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment* Genotype TIME_POINT
SA055364141002actsa21_1HMZ a7 HFD 35 weeks
SA055365141002actsa05_1HMZ a7 HFD 35 weeks
SA055366141002actsa27_1HMZ a7 HFD 35 weeks
SA055367141002actsa19_1HMZ a7 HFD 35 weeks
SA055368141001actsa24_1HMZ a7 HFD 35 weeks
SA055369141002actsa30_1HMZ a7 HFD 35 weeks
SA055370141002actsa16_1HMZ a7 HFD 35 weeks
SA055371141001actsa10_1HMZ a7 HFD 35 weeks
SA055372141001actsa48_1HMZ a7 LA-HFD 35 weeks
SA055373141001actsa05_1HMZ a7 LA-HFD 35 weeks
SA055374141002actsa17_1HMZ a7 LA-HFD 35 weeks
SA055375141001actsa49_1HMZ a7 LA-HFD 35 weeks
SA055376141001actsa30_1HMZ a7 LA-HFD 35 weeks
SA055377141002actsa15_1HMZ a7 LA-HFD 35 weeks
SA055378141002actsa06_1HMZ a7 LA-HFD 35 weeks
SA055379141002actsa12_1HMZ a7 LA-HFD 35 weeks
SA055380141001actsa01_1HMZ a7 Viv chow 35 weeks
SA055381141002actsa20_1HMZ a7 Viv chow 35 weeks
SA055382141001actsa06_1HMZ a7 Viv chow 35 weeks
SA055383141001actsa39_1HMZ a7 Viv chow 35 weeks
SA055384141001actsa04_1HMZ a7 Viv chow 35 weeks
SA055385141001actsa12_1HMZ a7 Viv chow 35 weeks
SA055386141002actsa14_1HMZ a7 Viv chow 35 weeks
SA055387141002actsa33_1HMZ a7 Viv chow 35 weeks
SA055388141001actsa35_1WT C57-Bl6 HFD 24 weeks
SA055389141001actsa16_1WT C57-Bl6 HFD 24 weeks
SA055390141001actsa46_1WT C57-Bl6 HFD 24 weeks
SA055391141002actsa23_1WT C57-Bl6 HFD 24 weeks
SA055392141002actsa25_1WT C57-Bl6 HFD 24 weeks
SA055393141001actsa26_1WT C57-Bl6 HFD 24 weeks
SA055394141001actsa29_1WT C57-Bl6 HFD 24 weeks
SA055395141002actsa09_1WT C57-Bl6 HFD 24 weeks
SA055396141002actsa31_1WT C57-Bl6 HFD 35 weeks
SA055397141002actsa03_1WT C57-Bl6 HFD 35 weeks
SA055398141002actsa28_1WT C57-Bl6 HFD 35 weeks
SA055399141001actsa19_1WT C57-Bl6 HFD 35 weeks
SA055400141001actsa37_1WT C57-Bl6 HFD 35 weeks
SA055401141001actsa18_1WT C57-Bl6 HFD 35 weeks
SA055402141002actsa01_1WT C57-Bl6 HFD 35 weeks
SA055403141001actsa31_1WT C57-Bl6 HFD 35 weeks
SA055404141001actsa23_1WT C57-Bl6 LA-HFD 24 weeks
SA055405141001actsa36_1WT C57-Bl6 LA-HFD 24 weeks
SA055406141002actsa02_1WT C57-Bl6 LA-HFD 24 weeks
SA055407141001actsa27_1WT C57-Bl6 LA-HFD 24 weeks
SA055408141002actsa22_1WT C57-Bl6 LA-HFD 24 weeks
SA055409141001actsa22_1WT C57-Bl6 LA-HFD 24 weeks
SA055410141002actsa26_1WT C57-Bl6 LA-HFD 24 weeks
SA055411141001actsa32_1WT C57-Bl6 LA-HFD 24 weeks
SA055412141001actsa43_1WT C57-Bl6 LA-HFD 35 weeks
SA055413141001actsa41_1WT C57-Bl6 LA-HFD 35 weeks
SA055414141001actsa13_1WT C57-Bl6 LA-HFD 35 weeks
SA055415141002actsa24_1WT C57-Bl6 LA-HFD 35 weeks
SA055416141001actsa50_1WT C57-Bl6 LA-HFD 35 weeks
SA055417141001actsa14_1WT C57-Bl6 LA-HFD 35 weeks
SA055418141002actsa10_1WT C57-Bl6 LA-HFD 35 weeks
SA055419141001actsa20_1WT C57-Bl6 LA-HFD 35 weeks
SA055420141001actsa08_1WT C57-Bl6 PL-HFD 24 weeks
SA055421141001actsa03_1WT C57-Bl6 PL-HFD 24 weeks
SA055422141002actsa07_2WT C57-Bl6 PL-HFD 24 weeks
SA055423141001actsa02_1WT C57-Bl6 PL-HFD 24 weeks
SA055424141002actsa32_1WT C57-Bl6 PL-HFD 24 weeks
SA055425141001actsa42_1WT C57-Bl6 PL-HFD 24 weeks
SA055426141001actsa09_1WT C57-Bl6 PL-HFD 24 weeks
SA055427141001actsa25_1WT C57-Bl6 PL-HFD 24 weeks
SA055428141002actsa04_1WT C57-Bl6 Viv chow 24 weeks
SA055429141002actsa13_1WT C57-Bl6 Viv chow 24 weeks
SA055430141001actsa17_1WT C57-Bl6 Viv chow 24 weeks
SA055431141002actsa29_1WT C57-Bl6 Viv chow 24 weeks
SA055432141001actsa44_1WT C57-Bl6 Viv chow 24 weeks
SA055433141001actsa28_1WT C57-Bl6 Viv chow 24 weeks
SA055434141001actsa07_1WT C57-Bl6 Viv chow 24 weeks
SA055435141001actsa38_1WT C57-Bl6 Viv chow 35 weeks
SA055436141001actsa34_1WT C57-Bl6 Viv chow 35 weeks
SA055437141001actsa11_1WT C57-Bl6 Viv chow 35 weeks
SA055438141002actsa08_1WT C57-Bl6 Viv chow 35 weeks
SA055439141001actsa15_1WT C57-Bl6 Viv chow 35 weeks
SA055440141001actsa40_1WT C57-Bl6 Viv chow 35 weeks
SA055441141001actsa47_1WT C57-Bl6 Viv chow 35 weeks
SA055442141001actsa33_1WT C57-Bl6 Viv chow 35 weeks
Showing results 1 to 79 of 79

Collection:

Collection ID:CO000961
Collection Summary:Liver tissue from mice on the diets for 24 weeks for metabolomic analysis was collected, rinsed in cold PBS, excess fluid was blotted with a kim-wipe and tissue was immediately snap frozen in liquid nitrogen before storage at -80°C. Blood was collected by cardiac puncture and centrifuged at 9 rcf for 5 min at 4°C. Plasma was stored immediately at -20°C.
Sample Type:Blood

Treatment:

Treatment ID:TR000981
Treatment Summary:Male C57/BL6N mice weaned at 3weeks of age were randomly assigned to one of the four diets: 1) VIV chow: normal rodent chow, low in fat and high in fiber 2) HFD: 40 kcal% coconut oil 3) LA-HFD: 40 kcal% total fat soybean oil diet (21 kcal% from coconut oil and 19 kcal% from soybean oil) 4) PL-HFD: 40 kcal% total fat Plenish oil diet (21 kcal% from coconut oil and 19 kcal% from Plenish oil)

Sample Preparation:

Sampleprep ID:SP000974
Sampleprep Summary:1. Switch on bath to pre-cool at –20°C (±2°C validity temperature range) 2. Gently rotate or aspirate the blood samples for about 10s to obtain a homogenised sample. 3. Aliquot 30μl of plasma sample to a 1.0 mL extraction solution. The extraction solution has to be prechilled using the ThermoElectron Neslab RTE 740 cooling bath set to -20°C. 4. Vortex the sample for about 10s and shake for 5 min at 4°C using the Orbital Mixing Chilling/Heating Plate. If you are using more than one sample, keep the rest of the sample on ice (chilled at <0°C with sodium chloride). 5. Centrifuge samples for 2min at 14000 rcf using the centrifuge Eppendorf 5415 D. 6. Aliquot two 450μL portions of the supernatant. One for analysis and one for a backup sample. Store the backup aliquot in -20°C freezer. 7. Evaporate one 450μL aliquots of the sample in the Labconco Centrivap cold trap concentrator to complete dryness. 8. The dried aliquot is then re-suspended with 450 μL 50% acetonitrile (degassed as given above). 9. Centrifuged for 2 min at 14000 rcf using the centrifuge Eppendorf 5415. 10. Remove supernatant to a new Eppendorf tube. 11. Evaporate the supernatant to dryness in the Labconco Centrivap cold trap concentrator. 12. Submit to derivatization.
Sampleprep Protocol Filename:SOP_Sample_preparation_of_blood_plasma_or_serum_samples_for_GCTOF_analysis.pdf

Combined analysis:

Analysis ID AN001522
Analysis type MS
Chromatography type GC
Chromatography system Agilent
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC Ion Trap
MS instrument name Varian 210-MS GC Ion Trap
Ion Mode POSITIVE
Units Counts

Chromatography:

Chromatography ID:CH001072
Instrument Name:Agilent
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Pressure:7.7 PSI (initial condition)
Column Temperature:50 - 330°C
Flow Rate:1 ml/min
Injection Temperature:50°C ramped to 250°C by 12°C/s
Sample Injection:0.5µl
Oven Temperature:50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min
Transferline Temperature:230°C
Washing Buffer:Ethyl Acetate
Sample Loop Size:30 m length x 0.25 mm internal diameter
Randomization Order:Excel generated
Chromatography Type:GC

MS:

MS ID:MS001402
Analysis ID:AN001522
Instrument Name:Varian 210-MS GC Ion Trap
Instrument Type:GC Ion Trap
MS Type:EI
Ion Mode:POSITIVE
Ion Source Temperature:250°C
Ionization Energy:70eV
Mass Accuracy:Nominal
Source Temperature:250°C
Scan Range Moverz:85-500
Scanning Cycle:17 Hz
Scanning Range:80-500 Da
Skimmer Voltage:1850 V
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