Summary of Study ST000660

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000461. The data can be accessed directly via it's Project DOI: 10.21228/M8G609 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST000660
Study Title	Omega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice (part I)
Study Summary	In this study we have compared the metabolic effects of conventional soybean oil to those of genetically modified Plenish soybean oil, that is low in linoleic acid and high in oleic acid. This work builds on our previous study showing that soybean oil, rich in polyunsaturated fats, is more obesogenic and diabetogenic than coconut oil, rich in saturated fats (PMID: 26200659). Here, in order to elucidate the mechanisms responsible for soybean oil induced obesity, we have performed the first ever metabolomics (in plasma and liver) and proteomics on the livers of mice fed the two soybean oil diets (plus those fed a high coconut oil and Viv chow diet). Our results show that the new high oleic soybean oil induces less obesity and adiposity than conventional soybean oil, but can cause hepatomegaly and liver dysfunction. Metabolomic analysis reveals that the hepatic and plasma metabolic profiles differ considerably between the two soybean oils. Hepatic C18 oxylipin metabolites of omega-6 (ω6) and omega-3 (ω3) fatty acids (linoleic and α-linolenic acid, respectively) in the cytochrome P450/soluble epoxide hydrolase pathway were found to correlate positively with obesity.
Institute	University of California, Davis
Department	Genome and Biomedical Sciences Facility
Laboratory	WCMC Metabolomics Core
Last Name	Fiehn
First Name	Oliver
Address	1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Email	ofiehn@ucdavis.edu
Phone	(530) 754-8258
Submit Date	2017-06-22
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2017-10-03
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000461
Project DOI:	doi: 10.21228/M8G609
Project Title:	Omega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice
Project Summary:	In this study we have compared the metabolic effects of conventional soybean oil to those of genetically modified Plenish soybean oil, that is low in linoleic acid and high in oleic acid. This work builds on our previous study showing that soybean oil, rich in polyunsaturated fats, is more obesogenic and diabetogenic than coconut oil, rich in saturated fats (PMID: 26200659). Here, in order to elucidate the mechanisms responsible for soybean oil induced obesity, we have performed the first ever metabolomics (in plasma and liver) and proteomics on the livers of mice fed the two soybean oil diets (plus those fed a high coconut oil and Viv chow diet). Our results show that the new high oleic soybean oil induces less obesity and adiposity than conventional soybean oil, but can cause hepatomegaly and liver dysfunction. Metabolomic analysis reveals that the hepatic and plasma metabolic profiles differ considerably between the two soybean oils. Hepatic C18 oxylipin metabolites of omega-6 (ω6) and omega-3 (ω3) fatty acids (linoleic and α-linolenic acid, respectively) in the cytochrome P450/soluble epoxide hydrolase pathway were found to correlate positively with obesity.
Institute:	University of California, Riverside
Department:	Cell Biology and Neuroscience
Last Name:	Sladek
First Name:	Frances
Address:	2115 Biological Sciences Building,University of California, Riverside, CA 92521-0314
Email:	frances.sladek@ucr.edu
Phone:	951-827-2264

Subject:

Subject ID:	SU000967
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57/BL6N
Gender:	Male
Animal Housing:	SPF facility
Animal Light Cycle:	12:12 h light-dark cycle
Animal Feed:	Ad libitum
Animal Water:	Ad libitum
Species Group:	Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment*	Genotype	TIME_POINT
SA055364	141002actsa21_1	HMZ a7	HFD	35 weeks
SA055365	141002actsa05_1	HMZ a7	HFD	35 weeks
SA055366	141002actsa27_1	HMZ a7	HFD	35 weeks
SA055367	141002actsa19_1	HMZ a7	HFD	35 weeks
SA055368	141001actsa24_1	HMZ a7	HFD	35 weeks
SA055369	141002actsa30_1	HMZ a7	HFD	35 weeks
SA055370	141002actsa16_1	HMZ a7	HFD	35 weeks
SA055371	141001actsa10_1	HMZ a7	HFD	35 weeks
SA055372	141001actsa48_1	HMZ a7	LA-HFD	35 weeks
SA055373	141001actsa05_1	HMZ a7	LA-HFD	35 weeks
SA055374	141002actsa17_1	HMZ a7	LA-HFD	35 weeks
SA055375	141001actsa49_1	HMZ a7	LA-HFD	35 weeks
SA055376	141001actsa30_1	HMZ a7	LA-HFD	35 weeks
SA055377	141002actsa15_1	HMZ a7	LA-HFD	35 weeks
SA055378	141002actsa06_1	HMZ a7	LA-HFD	35 weeks
SA055379	141002actsa12_1	HMZ a7	LA-HFD	35 weeks
SA055380	141001actsa01_1	HMZ a7	Viv chow	35 weeks
SA055381	141002actsa20_1	HMZ a7	Viv chow	35 weeks
SA055382	141001actsa06_1	HMZ a7	Viv chow	35 weeks
SA055383	141001actsa39_1	HMZ a7	Viv chow	35 weeks
SA055384	141001actsa04_1	HMZ a7	Viv chow	35 weeks
SA055385	141001actsa12_1	HMZ a7	Viv chow	35 weeks
SA055386	141002actsa14_1	HMZ a7	Viv chow	35 weeks
SA055387	141002actsa33_1	HMZ a7	Viv chow	35 weeks
SA055388	141001actsa35_1	WT C57-Bl6	HFD	24 weeks
SA055389	141001actsa16_1	WT C57-Bl6	HFD	24 weeks
SA055390	141001actsa46_1	WT C57-Bl6	HFD	24 weeks
SA055391	141002actsa23_1	WT C57-Bl6	HFD	24 weeks
SA055392	141002actsa25_1	WT C57-Bl6	HFD	24 weeks
SA055393	141001actsa26_1	WT C57-Bl6	HFD	24 weeks
SA055394	141001actsa29_1	WT C57-Bl6	HFD	24 weeks
SA055395	141002actsa09_1	WT C57-Bl6	HFD	24 weeks
SA055396	141002actsa31_1	WT C57-Bl6	HFD	35 weeks
SA055397	141002actsa03_1	WT C57-Bl6	HFD	35 weeks
SA055398	141002actsa28_1	WT C57-Bl6	HFD	35 weeks
SA055399	141001actsa19_1	WT C57-Bl6	HFD	35 weeks
SA055400	141001actsa37_1	WT C57-Bl6	HFD	35 weeks
SA055401	141001actsa18_1	WT C57-Bl6	HFD	35 weeks
SA055402	141002actsa01_1	WT C57-Bl6	HFD	35 weeks
SA055403	141001actsa31_1	WT C57-Bl6	HFD	35 weeks
SA055404	141001actsa23_1	WT C57-Bl6	LA-HFD	24 weeks
SA055405	141001actsa36_1	WT C57-Bl6	LA-HFD	24 weeks
SA055406	141002actsa02_1	WT C57-Bl6	LA-HFD	24 weeks
SA055407	141001actsa27_1	WT C57-Bl6	LA-HFD	24 weeks
SA055408	141002actsa22_1	WT C57-Bl6	LA-HFD	24 weeks
SA055409	141001actsa22_1	WT C57-Bl6	LA-HFD	24 weeks
SA055410	141002actsa26_1	WT C57-Bl6	LA-HFD	24 weeks
SA055411	141001actsa32_1	WT C57-Bl6	LA-HFD	24 weeks
SA055412	141001actsa43_1	WT C57-Bl6	LA-HFD	35 weeks
SA055413	141001actsa41_1	WT C57-Bl6	LA-HFD	35 weeks
SA055414	141001actsa13_1	WT C57-Bl6	LA-HFD	35 weeks
SA055415	141002actsa24_1	WT C57-Bl6	LA-HFD	35 weeks
SA055416	141001actsa50_1	WT C57-Bl6	LA-HFD	35 weeks
SA055417	141001actsa14_1	WT C57-Bl6	LA-HFD	35 weeks
SA055418	141002actsa10_1	WT C57-Bl6	LA-HFD	35 weeks
SA055419	141001actsa20_1	WT C57-Bl6	LA-HFD	35 weeks
SA055420	141001actsa08_1	WT C57-Bl6	PL-HFD	24 weeks
SA055421	141001actsa03_1	WT C57-Bl6	PL-HFD	24 weeks
SA055422	141002actsa07_2	WT C57-Bl6	PL-HFD	24 weeks
SA055423	141001actsa02_1	WT C57-Bl6	PL-HFD	24 weeks
SA055424	141002actsa32_1	WT C57-Bl6	PL-HFD	24 weeks
SA055425	141001actsa42_1	WT C57-Bl6	PL-HFD	24 weeks
SA055426	141001actsa09_1	WT C57-Bl6	PL-HFD	24 weeks
SA055427	141001actsa25_1	WT C57-Bl6	PL-HFD	24 weeks
SA055428	141002actsa04_1	WT C57-Bl6	Viv chow	24 weeks
SA055429	141002actsa13_1	WT C57-Bl6	Viv chow	24 weeks
SA055430	141001actsa17_1	WT C57-Bl6	Viv chow	24 weeks
SA055431	141002actsa29_1	WT C57-Bl6	Viv chow	24 weeks
SA055432	141001actsa44_1	WT C57-Bl6	Viv chow	24 weeks
SA055433	141001actsa28_1	WT C57-Bl6	Viv chow	24 weeks
SA055434	141001actsa07_1	WT C57-Bl6	Viv chow	24 weeks
SA055435	141001actsa38_1	WT C57-Bl6	Viv chow	35 weeks
SA055436	141001actsa34_1	WT C57-Bl6	Viv chow	35 weeks
SA055437	141001actsa11_1	WT C57-Bl6	Viv chow	35 weeks
SA055438	141002actsa08_1	WT C57-Bl6	Viv chow	35 weeks
SA055439	141001actsa15_1	WT C57-Bl6	Viv chow	35 weeks
SA055440	141001actsa40_1	WT C57-Bl6	Viv chow	35 weeks
SA055441	141001actsa47_1	WT C57-Bl6	Viv chow	35 weeks
SA055442	141001actsa33_1	WT C57-Bl6	Viv chow	35 weeks

Showing results 1 to 79 of 79

Showing results 1 to 79 of 79

Collection:

Collection ID:	CO000961
Collection Summary:	Liver tissue from mice on the diets for 24 weeks for metabolomic analysis was collected, rinsed in cold PBS, excess fluid was blotted with a kim-wipe and tissue was immediately snap frozen in liquid nitrogen before storage at -80°C. Blood was collected by cardiac puncture and centrifuged at 9 rcf for 5 min at 4°C. Plasma was stored immediately at -20°C.
Sample Type:	Blood

Treatment:

Treatment ID:	TR000981
Treatment Summary:	Male C57/BL6N mice weaned at 3weeks of age were randomly assigned to one of the four diets: 1) VIV chow: normal rodent chow, low in fat and high in fiber 2) HFD: 40 kcal% coconut oil 3) LA-HFD: 40 kcal% total fat soybean oil diet (21 kcal% from coconut oil and 19 kcal% from soybean oil) 4) PL-HFD: 40 kcal% total fat Plenish oil diet (21 kcal% from coconut oil and 19 kcal% from Plenish oil)

Sample Preparation:

Sampleprep ID:	SP000974
Sampleprep Summary:	1. Switch on bath to pre-cool at –20°C (±2°C validity temperature range) 2. Gently rotate or aspirate the blood samples for about 10s to obtain a homogenised sample. 3. Aliquot 30μl of plasma sample to a 1.0 mL extraction solution. The extraction solution has to be prechilled using the ThermoElectron Neslab RTE 740 cooling bath set to -20°C. 4. Vortex the sample for about 10s and shake for 5 min at 4°C using the Orbital Mixing Chilling/Heating Plate. If you are using more than one sample, keep the rest of the sample on ice (chilled at <0°C with sodium chloride). 5. Centrifuge samples for 2min at 14000 rcf using the centrifuge Eppendorf 5415 D. 6. Aliquot two 450μL portions of the supernatant. One for analysis and one for a backup sample. Store the backup aliquot in -20°C freezer. 7. Evaporate one 450μL aliquots of the sample in the Labconco Centrivap cold trap concentrator to complete dryness. 8. The dried aliquot is then re-suspended with 450 μL 50% acetonitrile (degassed as given above). 9. Centrifuged for 2 min at 14000 rcf using the centrifuge Eppendorf 5415. 10. Remove supernatant to a new Eppendorf tube. 11. Evaporate the supernatant to dryness in the Labconco Centrivap cold trap concentrator. 12. Submit to derivatization.
Sampleprep Protocol Filename:	SOP_Sample_preparation_of_blood_plasma_or_serum_samples_for_GCTOF_analysis.pdf

Combined analysis:

Analysis ID	AN001522
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent
Column	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type	EI
MS instrument type	GC Ion Trap
MS instrument name	Varian 210-MS GC Ion Trap
Ion Mode	POSITIVE
Units	Counts

Chromatography:

Chromatography ID:	CH001072
Instrument Name:	Agilent
Column Name:	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Pressure:	7.7 PSI (initial condition)
Column Temperature:	50 - 330°C
Flow Rate:	1 ml/min
Injection Temperature:	50°C ramped to 250°C by 12°C/s
Sample Injection:	0.5µl
Oven Temperature:	50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min
Transferline Temperature:	230°C
Washing Buffer:	Ethyl Acetate
Sample Loop Size:	30 m length x 0.25 mm internal diameter
Randomization Order:	Excel generated
Chromatography Type:	GC

MS:

MS ID:	MS001402
Analysis ID:	AN001522
Instrument Name:	Varian 210-MS GC Ion Trap
Instrument Type:	GC Ion Trap
MS Type:	EI
Ion Mode:	POSITIVE
Ion Source Temperature:	250°C
Ionization Energy:	70eV
Mass Accuracy:	Nominal
Source Temperature:	250°C
Scan Range Moverz:	85-500
Scanning Cycle:	17 Hz
Scanning Range:	80-500 Da
Skimmer Voltage:	1850 V