Summary of Study ST000662

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000465. The data can be accessed directly via it's Project DOI: 10.21228/M8Z60N This work is supported by NIH grant, U2C- DK119886.

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Study IDST000662
Study TitleLipidomics of hormone-sensitive lipase variants
Study TypeMS analysis
Study SummaryThe samples are frozen biopsies of whilte subcutaneous adipose tissues (50 mg). Samples were collected from 4 hormone-sensitive lipase (HSL) WT patients (adipose insulin sensitive), 3 HSL heterozygote patients and 1 HSL KO patient.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups3
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8Z60N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000465
Project DOI:doi: 10.21228/M8Z60N
Project Title:Hormone-sensitive lipase (HSL) knock-out
Project Type:MS analysis
Project Summary:Lipidomics in control, HSL ID, HSL DD
Institute:University of Maryland
Department:Medicine
Laboratory:Sztalryd-Woodle Lab
Last Name:Sztalryd-Woodle
First Name:Carole
Address:Baltimore, MD
Email:csztalry@grecc.umaryland.edu
Phone:410-706-4047

Subject:

Subject ID:SU000685
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA038150S00014864HSL heterozygote
SA038151S00014863HSL heterozygote
SA038152S00014862HSL heterozygote
SA038149S00014865HSL KO
SA038153S00014859WT
SA038154S00014860WT
SA038155S00014861WT
SA038156S00014858WT
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000679
Collection Summary:none
Sample Type:Adipose tissue

Treatment:

Treatment ID:TR000699
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000692
Sampleprep Summary:none
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001010 AN001011
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000725
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000905
Analysis ID:AN001010
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000906
Analysis ID:AN001011
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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