Summary of Study ST000665

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000468. The data can be accessed directly via it's Project DOI: 10.21228/M8K02X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000665
Study TitleNonalcoholic fatty liver disease (NAFLD) Studies
Study TypeMS analysis
Study SummaryDetermine the genetic mechanism of action by which variants near PNPLA3 and LYPLAL1 exert their effects on promoting NAFLD
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups2
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8K02X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000468
Project DOI:doi: 10.21228/M8K02X
Project Title:Nonalcoholic fatty liver disease (NAFLD) Studies
Project Type:MS analysis
Project Summary:Determine the genetic mechanism of action by which variants near PNPLA3 and LYPLAL1 exert their effects on promoting NAFLD
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Speliotes Lab
Last Name:Speliotes
First Name:Elizabeth
Address:Ann Arbor, MI
Email:espeliot@umich.edu
Phone:734-647-2964

Subject:

Subject ID:SU000688
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line
SA038362S00015866HepG2
SA038363S00015860HepG2
SA038364S00015865HepG2
SA038365S00015867HepG2
SA038366S00015861HepG2
SA038367S00015864HepG2
SA038368S00015862HepG2
SA038369S00015863HepG2
SA038370S00015878HuH-7
SA038371S00015877HuH-7
SA038372S00015879HuH-7
SA038373S00015881HuH-7
SA038374S00015876HuH-7
SA038375S00015880HuH-7
SA038376S00015871HuH-7
SA038377S00015869HuH-7
SA038378S00015868HuH-7
SA038379S00015870HuH-7
SA038380S00015872HuH-7
SA038381S00015874HuH-7
SA038382S00015873HuH-7
SA038383S00015875HuH-7
Showing results 1 to 22 of 22

Collection:

Collection ID:CO000682
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000702
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000695
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001016 AN001017
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000728
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000911
Analysis ID:AN001016
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000912
Analysis ID:AN001017
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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