Summary of Study ST000666

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000469. The data can be accessed directly via it's Project DOI: 10.21228/M8F61C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000666
Study TitleRat Rotator Cuff Lipidomics
Study TypeMS analysis
Study SummaryRats were subjected to bilateral rotator cuff tears of the right and left supraspinatus muscle. Muscles were harvested from each shoulder at 0, 10, 30, or 60 days post surgery.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups6
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8F61C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000469
Project DOI:doi: 10.21228/M8F61C
Project Title:Rat Rotator Cuff Lipidomics Study
Project Type:MS analysis
Project Summary:Shotgun Lipidomics of samples obtained from various timepoints of rotator cuff injury
Institute:University of Michigan
Department:Orthopaedic Surgery
Laboratory:Mendias Lab
Last Name:Mendias
First Name:Christopher
Address:Ann Arbor, MI
Email:cmendias@umich.edu
Phone:734-764-3250

Subject:

Subject ID:SU000689
Subject Type:RAT
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Subject type: RAT; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Side TIME_DAYS
SA038384S00016773L -
SA038385S00016779L -
SA038386S00016777L -
SA038387S00016781L -
SA038388S00016775L -
SA038389S00016785L 10
SA038390S00016783L 10
SA038391S00016791L 10
SA038392S00016787L 10
SA038393S00016789L 10
SA038394S00016797L 30
SA038395S00016801L 30
SA038396S00016799L 30
SA038397S00016795L 30
SA038398S00016793L 30
SA038399S00016805L 60
SA038400S00016803L 60
SA038401S00016807L 60
SA038402S00016809L 60
SA038403S00016811L 60
SA038404S00016778R -
SA038405S00016774R -
SA038406S00016780R -
SA038407S00016776R -
SA038408S00016782R -
SA038409S00016784R 10
SA038410S00016792R 10
SA038411S00016788R 10
SA038412S00016790R 10
SA038413S00016786R 10
SA038414S00016798R 30
SA038415S00016794R 30
SA038416S00016796R 30
SA038417S00016800R 30
SA038418S00016802R 30
SA038419S00016804R 60
SA038420S00016812R 60
SA038421S00016808R 60
SA038422S00016810R 60
SA038423S00016806R 60
Showing results 1 to 40 of 40

Collection:

Collection ID:CO000683
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000703
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000696
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001018 AN001019
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000729
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000913
Analysis ID:AN001018
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000914
Analysis ID:AN001019
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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