Summary of Study ST000667
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000470. The data can be accessed directly via it's Project DOI: 10.21228/M89G75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000667 |
| Study Title | Diabetic Microvascular Plasma/Tissue Lipidomics- Comparison |
| Study Type | MS analysis |
| Study Summary | Determining lipid composition of diabetic microvascular complication-prone tissues and comparing tissues levels to plasma levels. Samples are in addition to plasma and kidney tissue samples ran (shotgun lipidomics) in June-July 2014. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000470 |
| Project DOI: | doi: 10.21228/M89G75 |
| Project Title: | Diabetic Microvascular Plasma/Tissue Lipidomics- Comparison |
| Project Type: | MS analysis |
| Project Summary: | Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models |
| Institute: | University of Michigan |
| Department: | UMich Internal Medicine |
| Laboratory: | Pennathur Lab |
| Last Name: | Pennathur |
| First Name: | Subramaniam |
| Address: | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| Email: | spennath@umich.edu |
| Phone: | 734 615 5757 |
Subject:
| Subject ID: | SU000690 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | CONTROL_DIABETIC | Sample type |
|---|---|---|---|
| SA038424 | S00018519 | db/db diabetic | Kidney |
| SA038425 | S00018520 | db/db diabetic | Kidney |
| SA038426 | S00018521 | db/db diabetic | Kidney |
| SA038427 | S00018523 | db/db diabetic | Plasma |
| SA038428 | S00018525 | db/db diabetic | Plasma |
| SA038429 | S00018522 | db/db diabetic | Plasma |
| SA038430 | S00018524 | db/db diabetic | Plasma |
| SA038431 | S00018516 | db/db diabetic | Retina |
| SA038432 | S00018517 | db/db diabetic | Retina |
| SA038433 | S00018515 | db/db diabetic | Retina |
| SA038434 | S00018514 | db/db diabetic | Retina |
| SA038435 | S00018508 | db/db diabetic | Sciatic Nerve |
| SA038436 | S00018504 | db/db diabetic | Sciatic Nerve |
| SA038437 | S00018505 | db/db diabetic | Sciatic Nerve |
| SA038438 | S00018506 | db/db diabetic | Sciatic Nerve |
| SA038439 | S00018507 | db/db diabetic | Sciatic Nerve |
| SA038440 | S00018518 | db/m control | Kidney |
| SA038441 | S00018526 | db/m control | Plasma |
| SA038442 | S00018513 | db/m control | Retina |
| SA038443 | S00018510 | db/m control | Retina |
| SA038444 | S00018511 | db/m control | Retina |
| SA038445 | S00018509 | db/m control | Retina |
| SA038446 | S00018512 | db/m control | Retina |
| SA038447 | S00018500 | db/m control | Sciatic Nerve |
| SA038448 | S00018501 | db/m control | Sciatic Nerve |
| SA038449 | S00018503 | db/m control | Sciatic Nerve |
| SA038450 | S00018499 | db/m control | Sciatic Nerve |
| SA038451 | S00018502 | db/m control | Sciatic Nerve |
| Showing results 1 to 28 of 28 |
Collection:
| Collection ID: | CO000684 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000704 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000697 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001020 | AN001021 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000730 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000915 |
| Analysis ID: | AN001020 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000916 |
| Analysis ID: | AN001021 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
