Summary of Study ST000672
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000470. The data can be accessed directly via it's Project DOI: 10.21228/M89G75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000672 |
| Study Title | Diabetic Microvascular Plasma/Tissue Lipidomics- Comparison (part II) |
| Study Type | MS analysis |
| Study Summary | Plasma, kidney, sciatic nerve, and retina samples collected from control (db/m) and diabetic (db/db) mice. Samples snap frozen and stored at -80. Plasma volume measured and tissues weighed for lipid extraction. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 31 |
| Study Comments | Plasma and kidney samples were originally prepped in June 2014 and analyzed on our first TripleTOF. George Michailidis has requested they be re-analyzed to compare results between platforms. N/R #91-111 are new samples that will be prepared for this experiment (n = 40). WHEN ANALYZING: need to separate by tissue (P, K, N, R) for imputation of missing values. Want raw data and normalized to IS. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000470 |
| Project DOI: | doi: 10.21228/M89G75 |
| Project Title: | Diabetic Microvascular Plasma/Tissue Lipidomics- Comparison |
| Project Type: | MS analysis |
| Project Summary: | Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models |
| Institute: | University of Michigan |
| Department: | UMich Internal Medicine |
| Laboratory: | Pennathur Lab |
| Last Name: | Pennathur |
| First Name: | Subramaniam |
| Address: | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| Email: | spennath@umich.edu |
| Phone: | 734 615 5757 |
Subject:
| Subject ID: | SU000695 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | CONTROL_DIABETIC | Sample type |
|---|---|---|---|
| SA038576 | S00021651 | db/db diabetic | Kidney |
| SA038577 | S00021654 | db/db diabetic | Kidney |
| SA038578 | S00021656 | db/db diabetic | Kidney |
| SA038579 | S00021659 | db/db diabetic | Kidney |
| SA038580 | S00021658 | db/db diabetic | Kidney |
| SA038581 | S00021648 | db/db diabetic | Plasma |
| SA038582 | S00021649 | db/db diabetic | Plasma |
| SA038583 | S00021644 | db/db diabetic | Plasma |
| SA038584 | S00021642 | db/db diabetic | Plasma |
| SA038585 | S00021646 | db/db diabetic | Plasma |
| SA038586 | S00021678 | db/db diabetic | Retina |
| SA038587 | S00021699 | db/db diabetic | Retina |
| SA038588 | S00021697 | db/db diabetic | Retina |
| SA038589 | S00021679 | db/db diabetic | Retina |
| SA038590 | S00021696 | db/db diabetic | Retina |
| SA038591 | S00021675 | db/db diabetic | Retina |
| SA038592 | S00021677 | db/db diabetic | Retina |
| SA038593 | S00021695 | db/db diabetic | Retina |
| SA038594 | S00021676 | db/db diabetic | Retina |
| SA038595 | S00021698 | db/db diabetic | Retina |
| SA038596 | S00021689 | db/db diabetic | Sciatic Nerve |
| SA038597 | S00021668 | db/db diabetic | Sciatic Nerve |
| SA038598 | S00021685 | db/db diabetic | Sciatic Nerve |
| SA038599 | S00021687 | db/db diabetic | Sciatic Nerve |
| SA038600 | S00021688 | db/db diabetic | Sciatic Nerve |
| SA038601 | S00021667 | db/db diabetic | Sciatic Nerve |
| SA038602 | S00021669 | db/db diabetic | Sciatic Nerve |
| SA038603 | S00021666 | db/db diabetic | Sciatic Nerve |
| SA038604 | S00021665 | db/db diabetic | Sciatic Nerve |
| SA038605 | S00021686 | db/db diabetic | Sciatic Nerve |
| SA038606 | S00021650 | db/m control | Kidney |
| SA038607 | S00021655 | db/m control | Kidney |
| SA038608 | S00021653 | db/m control | Kidney |
| SA038609 | S00021657 | db/m control | Kidney |
| SA038610 | S00021652 | db/m control | Kidney |
| SA038611 | S00021641 | db/m control | Plasma |
| SA038612 | S00021643 | db/m control | Plasma |
| SA038613 | S00021640 | db/m control | Plasma |
| SA038614 | S00021647 | db/m control | Plasma |
| SA038615 | S00021645 | db/m control | Plasma |
| SA038616 | S00021693 | db/m control | Retina |
| SA038617 | S00021694 | db/m control | Retina |
| SA038618 | S00021692 | db/m control | Retina |
| SA038619 | S00021691 | db/m control | Retina |
| SA038620 | S00021690 | db/m control | Retina |
| SA038621 | S00021670 | db/m control | Retina |
| SA038622 | S00021672 | db/m control | Retina |
| SA038623 | S00021671 | db/m control | Retina |
| SA038624 | S00021674 | db/m control | Retina |
| SA038625 | S00021673 | db/m control | Retina |
| SA038626 | S00021663 | db/m control | Sciatic Nerve |
| SA038627 | S00021662 | db/m control | Sciatic Nerve |
| SA038628 | S00021661 | db/m control | Sciatic Nerve |
| SA038629 | S00021660 | db/m control | Sciatic Nerve |
| SA038630 | S00021664 | db/m control | Sciatic Nerve |
| SA038631 | S00021683 | db/m control | Sciatic Nerve |
| SA038632 | S00021681 | db/m control | Sciatic Nerve |
| SA038633 | S00021684 | db/m control | Sciatic Nerve |
| SA038634 | S00021682 | db/m control | Sciatic Nerve |
| SA038635 | S00021680 | db/m control | Sciatic Nerve |
| Showing results 1 to 60 of 60 |
Collection:
| Collection ID: | CO000689 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000709 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000702 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Chromatography:
| Chromatography ID: | CH000735 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001030 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000735 |
| Num Factors: | 8 |
| Num Metabolites: | 435 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
| Analysis ID: | AN001031 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000735 |
| Num Factors: | 8 |
| Num Metabolites: | 657 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
