Summary of Study ST000675

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000477. The data can be accessed directly via it's Project DOI: 10.21228/M8D89S This work is supported by NIH grant, U2C- DK119886.

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Study IDST000675
Study TitleEffects of rosiglitazone treatment on lipid composition
Study TypeMS analysis
Study SummaryReprograming of 'white' to 'brite' adipocytes with higher oxidative capacity and improved endocrine function represents a potentially important approach to address the dysfunctional adipocyte phenotypes in obesity. We find that chronic treatment with the PPAR? agonist (rosiglitazone, 1 uM for 7days in vitro) in white human adipose tissue induced metabolic changes. Our trancriptome analysis showed that higher mitochondrial and peroxisomal fatty acid oxidation pathways and other genes involved in lipid metabolism including (re)esterification are induced by rosiglitazone treatment. To understand the biochemical basis of brite vs. white human adipocytes, we will perform comprehensive metabolomic profiling of control and rosiglitazone treated tissues using unbiased lipidomics approach.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups2
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8D89S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000477
Project DOI:doi: 10.21228/M8D89S
Project Title:Effects of rosiglitazone treatment on lipid composition
Project Type:MS analysis
Project Summary:rosiglitazone treatment of human adipose tissue
Institute:Boston University
Department:Department of Medicine
Laboratory:Lee, Mi-Jeong Lab
Last Name:Lee
First Name:Mi-Jeong
Address:Boston, MA
Email:lee.mijeong@gmail.com
Phone:617-429-2669

Subject:

Subject ID:SU000698
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id TYPE
SA038679S00022677control
SA038680S00022676control
SA038681S00022672control
SA038682S00022665control
SA038683S00022668control
SA038684S00022669control
SA038685S00022664control
SA038686S00022673control
SA038671S00022670Rosi
SA038672S00022671Rosi
SA038673S00022674Rosi
SA038674S00022675Rosi
SA038675S00022678Rosi
SA038676S00022679Rosi
SA038677S00022667Rosi
SA038678S00022666Rosi
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000692
Collection Summary:-
Sample Type:Adipose tissue

Treatment:

Treatment ID:TR000712
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000705
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001036 AN001037
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000738
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000931
Analysis ID:AN001036
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000932
Analysis ID:AN001037
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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