Summary of Study ST000676

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000478. The data can be accessed directly via it's Project DOI: 10.21228/M88G7V This work is supported by NIH grant, U2C- DK119886.

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Study IDST000676
Study TitleEffect of high fat diet and streptozotocin treatment on neuropathy
Study TypeMS analysis
Study SummaryC57BL6 male mice were fed either a control 10% fat (RD D12540-B) or a high fat 60% fat (RD 12492) diet from 5 wk age. STZ was given to some groups at 12 wk age. Chow was reversed in some groups (DR) from 60% HF to 10 % HF at 16 wk age. Mice were euthanized at either 16 wk or 24 wk age. Neuropathy phenotyping occured prior to all harvests.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups11
Total Subjects107
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M88G7V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000478
Project DOI:doi: 10.21228/M88G7V
Project Title:Effect of high fat diet and streptozotocin treatment on neuropathy
Project Type:MS analysis
Project Summary:Ctrl,HF and DR mice: scn,drg,plasma for lipidomics
Institute:University of Michigan
Department:Neurology
Laboratory:Feldman Lab
Last Name:Feldman
First Name:Eva
Address:Ann Arbor, MI
Email:efeldman@umich.edu
Phone:734-763-7274

Subject:

Subject ID:SU000699
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id AGE DIET SAMPLE_TYPE
SA038687S0002431416 week Control Dorsal root ganglia
SA038688S0002431216 week Control Dorsal root ganglia
SA038689S0002432016 week Control Dorsal root ganglia
SA038690S0002431116 week Control Dorsal root ganglia
SA038691S0002431516 week Control Dorsal root ganglia
SA038692S0002431316 week Control Dorsal root ganglia
SA038693S0002431616 week Control Dorsal root ganglia
SA038694S0002431816 week Control Dorsal root ganglia
SA038695S0002431916 week Control Dorsal root ganglia
SA038696S0002431716 week Control Dorsal root ganglia
SA038697S0002439516 week Control plasma
SA038698S0002439416 week Control plasma
SA038699S0002439216 week Control plasma
SA038700S0002439616 week Control plasma
SA038701S0002439316 week Control plasma
SA038702S0002439116 week Control plasma
SA038703S0002440016 week Control plasma
SA038704S0002439916 week Control plasma
SA038705S0002439816 week Control plasma
SA038706S0002439716 week Control plasma
SA038766S0002423516 week control Sciatic nerve
SA038767S0002423416 week control Sciatic nerve
SA038768S0002423216 week control Sciatic nerve
SA038769S0002423616 week control Sciatic nerve
SA038770S0002423316 week control Sciatic nerve
SA038771S0002424016 week control Sciatic nerve
SA038772S0002423716 week control Sciatic nerve
SA038773S0002423916 week control Sciatic nerve
SA038774S0002423116 week control Sciatic nerve
SA038775S0002423816 week control Sciatic nerve
SA038736S0002433616 week HF Dorsal root ganglia
SA038737S0002433716 week HF Dorsal root ganglia
SA038738S0002433816 week HF Dorsal root ganglia
SA038739S0002433516 week HF Dorsal root ganglia
SA038740S0002433416 week HF Dorsal root ganglia
SA038741S0002433116 week HF Dorsal root ganglia
SA038742S0002433216 week HF Dorsal root ganglia
SA038743S0002433316 week HF Dorsal root ganglia
SA038744S0002434016 week HF Dorsal root ganglia
SA038745S0002433916 week HF Dorsal root ganglia
SA038756S0002441616 week HF plasma
SA038757S0002441816 week HF plasma
SA038758S0002442016 week HF plasma
SA038759S0002441316 week HF plasma
SA038760S0002441216 week HF plasma
SA038761S0002441716 week HF plasma
SA038762S0002441516 week HF plasma
SA038763S0002441416 week HF plasma
SA038764S0002441916 week HF plasma
SA038765S0002441116 week HF plasma
SA038746S0002425516 week HF Sciatic nerve
SA038747S0002425416 week HF Sciatic nerve
SA038748S0002425316 week HF Sciatic nerve
SA038749S0002425616 week HF Sciatic nerve
SA038750S0002425116 week HF Sciatic nerve
SA038751S0002426016 week HF Sciatic nerve
SA038752S0002425916 week HF Sciatic nerve
SA038753S0002425816 week HF Sciatic nerve
SA038754S0002425216 week HF Sciatic nerve
SA038755S0002425716 week HF Sciatic nerve
SA038707S0002435816 week HF STZ Dorsal root ganglia
SA038708S0002435416 week HF STZ Dorsal root ganglia
SA038709S0002435316 week HF STZ Dorsal root ganglia
SA038710S0002436016 week HF STZ Dorsal root ganglia
SA038711S0002435116 week HF STZ Dorsal root ganglia
SA038712S0002435516 week HF STZ Dorsal root ganglia
SA038713S0002435216 week HF STZ Dorsal root ganglia
SA038714S0002435916 week HF STZ Dorsal root ganglia
SA038715S0002435716 week HF STZ Dorsal root ganglia
SA038716S0002435616 week HF STZ Dorsal root ganglia
SA038727S0002443416 week HF STZ plasma
SA038728S0002443516 week HF STZ plasma
SA038729S0002443616 week HF STZ plasma
SA038730S0002443316 week HF STZ plasma
SA038731S0002443116 week HF STZ plasma
SA038732S0002443016 week HF STZ plasma
SA038733S0002442916 week HF STZ plasma
SA038734S0002443716 week HF STZ plasma
SA038735S0002443216 week HF STZ plasma
SA038717S0002427416 week HF STZ Sciatic nerve
SA038718S0002427216 week HF STZ Sciatic nerve
SA038719S0002427516 week HF STZ Sciatic nerve
SA038720S0002427316 week HF STZ Sciatic nerve
SA038721S0002427816 week HF STZ Sciatic nerve
SA038722S0002427116 week HF STZ Sciatic nerve
SA038723S0002428016 week HF STZ Sciatic nerve
SA038724S0002427916 week HF STZ Sciatic nerve
SA038725S0002427616 week HF STZ Sciatic nerve
SA038726S0002427716 week HF STZ Sciatic nerve
SA038776S0002432724 week Control Dorsal root ganglia
SA038777S0002432824 week Control Dorsal root ganglia
SA038778S0002432924 week Control Dorsal root ganglia
SA038779S0002432624 week Control Dorsal root ganglia
SA038780S0002433024 week Control Dorsal root ganglia
SA038781S0002432524 week Control Dorsal root ganglia
SA038782S0002432224 week Control Dorsal root ganglia
SA038783S0002432124 week Control Dorsal root ganglia
SA038784S0002432324 week Control Dorsal root ganglia
SA038785S0002432424 week Control Dorsal root ganglia
SA038786S0002440324 week Control plasma
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Collection:

Collection ID:CO000693
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000713
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000706
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001038 AN001039
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000739
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000933
Analysis ID:AN001038
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000934
Analysis ID:AN001039
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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