Summary of Study ST000677
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000479. The data can be accessed directly via it's Project DOI: 10.21228/M84S5Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000677 |
| Study Title | Nonalcoholic steatohepatitis (NASH) changes with S.Q. Leptin administrations |
| Study Type | MS analysis |
| Study Summary | Samples are from individuals with documented fatty liver disease (NAFLD) from whom serum was drawn at baseline and after 1 and 12 months of S.Q. Leptin administrations. The effect of leptin on degree of steatosis and inflammation was assessed in liver biopsies at baseline and following 1 year of treatment. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000479 |
| Project DOI: | doi: 10.21228/M84S5Z |
| Project Title: | Nonalcoholic steatohepatitis (NASH) changes with S.Q. Leptin administrations |
| Project Type: | MS analysis |
| Project Summary: | Nonalcoholic steatohepatitis (NASH) Studies |
| Institute: | University of Michigan |
| Department: | Internal Medicine |
| Laboratory: | Burant Lab |
| Last Name: | Burant |
| First Name: | Chuck |
| Address: | 1500 E. Medical Center Drive Ann Arbor, MI 48109 |
| Email: | burantc@umich.edu |
| Phone: | 734 615-3481 |
Subject:
| Subject ID: | SU000700 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | visit |
|---|---|---|
| SA038923 | S00025126 | closeout |
| SA038924 | S00025129 | closeout |
| SA038925 | S00025132 | closeout |
| SA038926 | S00025135 | closeout |
| SA038927 | S00025124 | initial visit |
| SA038928 | S00025121 | initial visit |
| SA038929 | S00025118 | intitial visit |
| SA038930 | S00025115 | intitial visit |
| SA038931 | S00025131 | month 1 |
| SA038932 | S00025128 | month 1 |
| SA038933 | S00025134 | month 1 |
| SA038934 | S00025125 | month 1 |
| SA038935 | S00025122 | month 1 |
| SA038936 | S00025116 | month 1 |
| SA038937 | S00025119 | month 1 |
| SA038938 | S00025117 | month 12 |
| SA038939 | S00025120 | month 12 |
| SA038940 | S00025123 | month 12 |
| SA038941 | S00025130 | visit 1 |
| SA038942 | S00025127 | visit 1 |
| SA038943 | S00025133 | visit 1 |
| Showing results 1 to 21 of 21 |
Collection:
| Collection ID: | CO000694 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000714 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000707 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001040 | AN001041 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000740 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000935 |
| Analysis ID: | AN001040 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000936 |
| Analysis ID: | AN001041 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
