Summary of Study ST000681
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000481. The data can be accessed directly via it's Project DOI: 10.21228/M8W902 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000681 |
| Study Title | Retinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO - TY and LysM cre) |
| Study Type | MS analysis |
| Study Summary | ABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue. T:RPE specific, L:macrophage specific. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 5 |
| Total Subjects | 16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000481 |
| Project DOI: | doi: 10.21228/M8W902 |
| Project Title: | Retinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO) |
| Project Type: | MS analysis |
| Project Summary: | ABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue. |
| Institute: | Washington University in St. Louis |
| Department: | Department of Ophthalmology and visual sicences |
| Laboratory: | Apte Lab |
| Last Name: | Ban |
| First Name: | Norimitsu |
| Address: | 660 S. Euclid Ave. Box 8118 St. Louis, MO 63110 |
| Email: | BanN@vision.wustl.edu |
| Phone: | 314-362-6690 |
Subject:
| Subject ID: | SU000704 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | CRE | SEX |
|---|---|---|---|
| SA039003 | S00027118 | LysM Cre | f |
| SA039004 | S00027117 | LysM Cre | f |
| SA039005 | S00027116 | LysM Cre | f |
| SA039006 | S00027115 | LysM Cre | f |
| SA039011 | S00027114 | negative | f |
| SA039012 | S00027105 | negative | f |
| SA039013 | S00027104 | negative | f |
| SA039014 | S00027111 | negative | m |
| SA039015 | S00027106 | negative | m |
| SA039016 | S00027103 | negative | m |
| SA039017 | S00027112 | negative | m |
| SA039018 | S00027113 | negative | m |
| SA039007 | S00027108 | Tyrosinase Cre | f |
| SA039008 | S00027109 | Tyrosinase Cre | m |
| SA039009 | S00027107 | Tyrosinase Cre | m |
| SA039010 | S00027110 | Tyrosinase Cre | m |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000698 |
| Collection Summary: | - |
| Sample Type: | Retina |
Treatment:
| Treatment ID: | TR000718 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000711 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001048 | AN001049 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000744 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000943 |
| Analysis ID: | AN001048 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000944 |
| Analysis ID: | AN001049 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
