Summary of Study ST000681

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000481. The data can be accessed directly via it's Project DOI: 10.21228/M8W902 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000681
Study TitleRetinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO - TY and LysM cre)
Study TypeMS analysis
Study SummaryABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue. T:RPE specific, L:macrophage specific.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups5
Total Subjects16
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8W902
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000481
Project DOI:doi: 10.21228/M8W902
Project Title:Retinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO)
Project Type:MS analysis
Project Summary:ABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue.
Institute:Washington University in St. Louis
Department:Department of Ophthalmology and visual sicences
Laboratory:Apte Lab
Last Name:Ban
First Name:Norimitsu
Address:660 S. Euclid Ave. Box 8118 St. Louis, MO 63110
Email:BanN@vision.wustl.edu
Phone:314-362-6690

Subject:

Subject ID:SU000704
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id CRE SEX
SA039003S00027118LysM Cre f
SA039004S00027117LysM Cre f
SA039005S00027116LysM Cre f
SA039006S00027115LysM Cre f
SA039011S00027114negative f
SA039012S00027105negative f
SA039013S00027104negative f
SA039014S00027111negative m
SA039015S00027106negative m
SA039016S00027103negative m
SA039017S00027112negative m
SA039018S00027113negative m
SA039007S00027108Tyrosinase Cre f
SA039008S00027109Tyrosinase Cre m
SA039009S00027107Tyrosinase Cre m
SA039010S00027110Tyrosinase Cre m
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000698
Collection Summary:-
Sample Type:Retina

Treatment:

Treatment ID:TR000718
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000711
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Chromatography:

Chromatography ID:CH000744
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN001048
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000744
Num Factors:5
Num Metabolites:125
Rt Units:Minutes
Units:peak area normalized
  
Analysis ID:AN001049
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000744
Num Factors:5
Num Metabolites:174
Rt Units:Minutes
Units:peak area normalized
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