Summary of Study ST000682

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000481. The data can be accessed directly via it's Project DOI: 10.21228/M8W902 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000682
Study TitleRetinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO - TY and LysM cre) - part II
Study TypeMS analysis
Study SummaryABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue. R:Rod specific, C:Cone specific.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups5
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8W902
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000481
Project DOI:doi: 10.21228/M8W902
Project Title:Retinal tissue knockout to study efflux of cholesterol (AGCA1/G1 double KO)
Project Type:MS analysis
Project Summary:ABCA1 and AGCG1, which is important gene to efflux the cholesterol from the cell/tissue, are knocked out in specific retinal tissue.
Institute:Washington University in St. Louis
Department:Department of Ophthalmology and visual sicences
Laboratory:Apte Lab
Last Name:Ban
First Name:Norimitsu
Address:660 S. Euclid Ave. Box 8118 St. Louis, MO 63110
Email:BanN@vision.wustl.edu
Phone:314-362-6690

Subject:

Subject ID:SU000705
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id CRE SEX
SA039019S00027129Cone Cre f
SA039020S00027130Cone Cre f
SA039021S00027127Cone Cre m
SA039022S00027128Cone Cre m
SA039027S00027121negative f
SA039028S00027120negative m
SA039029S00027122negative m
SA039030S00027119negative m
SA039023S00027126Rod Cre f
SA039024S00027125Rod Cre f
SA039025S00027124Rod Cre f
SA039026S00027123Rod Cre f
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000699
Collection Summary:-
Sample Type:Retina

Treatment:

Treatment ID:TR000719
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000712
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Chromatography:

Chromatography ID:CH000745
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN001050
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000745
Num Factors:5
Num Metabolites:109
Rt Units:Minutes
Units:peak area normalized
  
Analysis ID:AN001051
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000745
Num Factors:5
Num Metabolites:135
Rt Units:Minutes
Units:peak area normalized
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